CAJ | 학술논문

目的:调查兰州市产妇念珠菌的定殖以及母婴间念珠菌的传播状况.方法: 采集产妇的阴道分泌物及口腔分泌物样本(共104×2 例),及其新生婴儿的口腔分泌物样本(共104 例).采用CHROMagar念珠菌显色培养基进行培养、分离及鉴定,同时应用分子生物学技术对结果进行验证鉴定.结果:312 例样本(母亲104×2,新生儿104)中81 例分离培养出念珠菌,产妇阴道念珠菌检测阳性率39.42 %(41 例),产妇口腔念珠菌阳性率33.65%(35 例),其中21.15%(22 例)阴道与口腔同时阳性; 新生儿口腔念珠菌培养阳性率为4.81%(5 例).检出阳性样本中菌种分布为,白色念珠菌53 株,光滑念珠菌33 株,克柔念珠菌2 株,热带念珠菌1 株.有2 对母婴同时分离出白色念珠菌,经PCR检测,其基因型相同.结论:兰州市新生儿念珠菌检出率以及母婴间念珠菌的传播率较其他地区高.新生儿念珠菌感染与念珠菌的水平传播和垂直传播都相关.监控医院内念珠菌的传播及预防母亲产前阴道念珠菌感染可减少新生儿念珠菌感染的可能性.
목적:조사란주시산부념주균적정식이급모영간념주균적전파상황.방법: 채집산부적음도분비물급구강분비물양본(공104×2 례),급기신생영인적구강분비물양본(공104 례).채용CHROMagar념주균현색배양기진행배양、분리급감정,동시응용분자생물학기술대결과진행험증감정.결과:312 례양본(모친104×2,신생인104)중81 례분리배양출념주균,산부음도념주균검측양성솔39.42 %(41 례),산부구강념주균양성솔33.65%(35 례),기중21.15%(22 례)음도여구강동시양성; 신생인구강념주균배양양성솔위4.81%(5 례).검출양성양본중균충분포위,백색념주균53 주,광활념주균33 주,극유념주균2 주,열대념주균1 주.유2 대모영동시분리출백색념주균,경PCR검측,기기인형상동.결론:란주시신생인념주균검출솔이급모영간념주균적전파솔교기타지구고.신생인념주균감염여념주균적수평전파화수직전파도상관.감공의원내념주균적전파급예방모친산전음도념주균감염가감소신생인념주균감염적가능성.
Objective: To investigate the candidal infection status in puerperas in Lanzhou, and the candidal transmission from mothers to their newborn infants. Methods: Vaginal fluid and saliva samples from 104 puerperas, as well as 104 saliva samples from their newborn infants were collected. The Candida species were cultured, isolated and identified using CHROMagar media. Further identification was done using molecular biological method. Results; In 81 of 312 specimens (104 x2 from mothers and 104 from infants), Candida species were found. 39.42% (41 cases) was observed in the vaginal fluid and 33.65% (35 cases) was in saliva of puerperas respectively, and 21. 15% (22 cases) in both vagina and oral cavity. 4.81% (5 cases) was found in oral cavities of newborn infants. The distribution of Candida species were 53 Candida albicans, 33 Candida glabrata, 2 Candida krusei and 1 Candida tropical. In 2 pairs of mother-infant, the same genotype of Candida ablicans was identified using PCR method. Conclusion; The Candida detection rate of newborn infants and transmission rate from mothers to their neonates in Lanzhou are higher than that reported in other areas. The colonization of Candida in newborn infants is relevant to both horizontal and vertical transmission. It can decrease the possibility of Candidal infection in newborn infants by controlling the Candidal transmission in hospital and preventing the infection in pregnant women.