CAJ | 학술논문

目的探讨脑胶质瘤SHG-44细胞株照射子代生长特性及辐射敏感性变化.方法培养人脑胶质瘤SHG-44细胞株照射后的存活子代细胞,测定其群体倍增时间,并进行集落形成实验和流式细胞仪检测,分析其辐射敏感性和细胞周期变化.结果 SHG-44细胞群体倍增时间为(22.78±2.61)h,SHG-44细胞经6 MV X射线10 Gy照射后存活子代SHG-44.10细胞群体倍增时间为(30.46±2.73)h(F=7.878,P<0.05).SHG-44细胞和SHG-44-10细胞再次2 Gy照射后的存活分数分别为70.8%、80.6%.与SHG-44细胞相比,SHG-44-10细胞在G2/M期比例减少,S期比例增多.结论 SHG-44细胞照射后存活子代细胞增殖延缓,辐射敏感性下降.
목적 탐토뇌효질류SHG-44세포주조사자대생장특성급복사민감성변화.방법 배양인뇌효질류SHG-44세포주조사후적존활자대세포,측정기군체배증시간,병진행집락형성실험화류식세포의검측,분석기복사민감성화세포주기변화.결과 SHG-44세포군체배증시간위(22.78±2.61)h,SHG-44세포경6 MV X사선10 Gy조사후존활자대SHG-44.10세포군체배증시간위(30.46±2.73)h(F=7.878,P<0.05).SHG-44세포화SHG-44-10세포재차2 Gy조사후적존활분수분별위70.8%、80.6%.여SHG-44세포상비,SHG-44-10세포재G2/M기비례감소,S기비례증다.결론 SHG-44세포조사후존활자대세포증식연완,복사민감성하강.
Objective To investigate the radiosensitivity and growth features of the progeny of irradiated human glioma cell line SHG-44.Methods The SHG-44 cells were irradiated with 6 MV X ray and the progeny of the cells were cultured.The population doubling time (PDT) was detected pre-and post-irradiation.The radiosensitivity of the progeny of irradiated human glioma cell line SHG-44 was measured by clone forming assay.The cell cycle distribution was analysed by flow cytometry.Results The PDT of SHG-44 cells before irradiation was (22.78±2.61) h.The PDT of irradiated SHG-44 cells at dose of 10 Gy were (30.46±2.73) h (F=7.878,P<0.05).The surviving fraction of SHG-44 cell was 70.8%,SHG-44-10 cell was 80.6%.SHG-44 cells showed significant GriM phase decrease and S phase arrest after irradiation.Conclusion Growth delay and declined radiosensitivity are confirmed in the progeny of irradiated SHG-44 cells.