中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2010年
12期
1287-1290
,共4页
何爱文%杨婷%陈寿权%李章平%李惠萍%黄唯佳%程俊彦%章杰%晏平%王万铁
何愛文%楊婷%陳壽權%李章平%李惠萍%黃唯佳%程俊彥%章傑%晏平%王萬鐵
하애문%양정%진수권%리장평%리혜평%황유가%정준언%장걸%안평%왕만철
心肺复苏%脑红蛋白%神经功能评分%氯化血红素%脑损伤%大鼠
心肺複囌%腦紅蛋白%神經功能評分%氯化血紅素%腦損傷%大鼠
심폐복소%뇌홍단백%신경공능평분%록화혈홍소%뇌손상%대서
Cardiopulmonary resuscitation%Neuroglobin%Neurodeficit score%IIemin%Cerebral injury%Rats
目的 观察心肺复苏(CPR)后大鼠脑皮质脑红蛋白(NGB)表达、神经功能评分(NDS)、大脑皮质病理的变化及氯化血红素(Hemin)干预的影响.方法 成年雄性SD大鼠120只,随机(随机数字法)分为3组(n=40):对照组(A)、复苏组(B)、药物组(C),建立窒息致大鼠心搏骤停CPR模型,观测大鼠自主循环恢复(ROSC)后各时间点(0.5 h,3 h,6 h,12 h,24 h)NGB表达、NDS及大脑皮质病理改变.实验数据作单因素方差分析,组间比较用Tukey检验.结果 与A组同时间点比较,B组ROSC后12 h,24 h NGB表达显著升高(P＜0.05或P＜0.01),ROSC后各时间点NDS显著降低(P＜0.01)、脑组织形态学明显异常;C组ROSC后6 h,12 h,24 h NGB表达显著升高(P＜0.05或P＜0.01),ROSC后3 h,6 h,12 h NDS显著降低(P＜0.01).与B组同时间点比较,C组ROSC后12 h,24 hNGB表达和NDS均显著升高(P＜0.01),ROSC后脑组织形态学异常明显减轻.结论 大鼠CPR后大脑皮质区NGB表达增加、NDS降低、脑组织形态学明显异常,Hemin能增加大脑皮层NGB表达、提高NDS、减轻皮层病理损伤,具有一定的脑保护作用.
目的 觀察心肺複囌(CPR)後大鼠腦皮質腦紅蛋白(NGB)錶達、神經功能評分(NDS)、大腦皮質病理的變化及氯化血紅素(Hemin)榦預的影響.方法 成年雄性SD大鼠120隻,隨機(隨機數字法)分為3組(n=40):對照組(A)、複囌組(B)、藥物組(C),建立窒息緻大鼠心搏驟停CPR模型,觀測大鼠自主循環恢複(ROSC)後各時間點(0.5 h,3 h,6 h,12 h,24 h)NGB錶達、NDS及大腦皮質病理改變.實驗數據作單因素方差分析,組間比較用Tukey檢驗.結果 與A組同時間點比較,B組ROSC後12 h,24 h NGB錶達顯著升高(P＜0.05或P＜0.01),ROSC後各時間點NDS顯著降低(P＜0.01)、腦組織形態學明顯異常;C組ROSC後6 h,12 h,24 h NGB錶達顯著升高(P＜0.05或P＜0.01),ROSC後3 h,6 h,12 h NDS顯著降低(P＜0.01).與B組同時間點比較,C組ROSC後12 h,24 hNGB錶達和NDS均顯著升高(P＜0.01),ROSC後腦組織形態學異常明顯減輕.結論 大鼠CPR後大腦皮質區NGB錶達增加、NDS降低、腦組織形態學明顯異常,Hemin能增加大腦皮層NGB錶達、提高NDS、減輕皮層病理損傷,具有一定的腦保護作用.
목적 관찰심폐복소(CPR)후대서뇌피질뇌홍단백(NGB)표체、신경공능평분(NDS)、대뇌피질병리적변화급록화혈홍소(Hemin)간예적영향.방법 성년웅성SD대서120지,수궤(수궤수자법)분위3조(n=40):대조조(A)、복소조(B)、약물조(C),건립질식치대서심박취정CPR모형,관측대서자주순배회복(ROSC)후각시간점(0.5 h,3 h,6 h,12 h,24 h)NGB표체、NDS급대뇌피질병리개변.실험수거작단인소방차분석,조간비교용Tukey검험.결과 여A조동시간점비교,B조ROSC후12 h,24 h NGB표체현저승고(P＜0.05혹P＜0.01),ROSC후각시간점NDS현저강저(P＜0.01)、뇌조직형태학명현이상;C조ROSC후6 h,12 h,24 h NGB표체현저승고(P＜0.05혹P＜0.01),ROSC후3 h,6 h,12 h NDS현저강저(P＜0.01).여B조동시간점비교,C조ROSC후12 h,24 hNGB표체화NDS균현저승고(P＜0.01),ROSC후뇌조직형태학이상명현감경.결론 대서CPR후대뇌피질구NGB표체증가、NDS강저、뇌조직형태학명현이상,Hemin능증가대뇌피층NGB표체、제고NDS、감경피층병리손상,구유일정적뇌보호작용.
Objective To study the effect of Hemin on the level of neuroglobin (NGB) in cerebral cortex,neurodeficit score (NDS) and pathological changes in cerebral cortex after cardiopulmonary resuscitation (CPR) in rats. Method A total of 120 male Sprague Dawley(SD) rats were divided randomly into control group(A), CPR group(B) and Hemin group(C). The animal model of cardiac arrest (CA) induced by asphyxia was established and CPR was performed. The NGB level in cerebral cortex, NDS and pathological changes in cerebral cortex were examined 0.5 h,3 h,6 h, 12 h, 24 h after restoration of spontaneous circulation (ROSC) in each group. Experimental data were analyzed by using one-factor analysis of variance and Tukey test. Results In comparison with group A, the levels of NGB were significantly higher 12 h,and 24 h after ROSC (P ＜0.05 and P ＜0.01), the values of NDS were significantly lower at each interval after ROSC ( P ＜ 0.01 ) ,and the pathological changes were more severe at each interval after ROSC in group B. In comparison with group A, the levels of NGB were significantly higher 6 h, 12 h and 24 h after ROSC ( P ＜ 0.05 and P ＜ 0.01 ), and the values of NDS were significantly lower 3 h, 6 h, 12 h after ROSC ( P ＜ 0.01) in group C. In comparison with group B, the levels of NGB were significantly higher 12 h and 24 h after ROSC, the values of NDS were significantly higher 12 h and 24 h after ROSC, and the pathological changes were less in group C. Conclusions The NGB level increased in cerebral cortex, the NDS level decreased and severe pathological changes occurred in rats after CPR. The hemin treatment up-regulated the level of NGB, improved the NDS, mitigated pathological changes, alleviating the cerebral injury after CPR.
