中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2012年
7期
718-724
,共7页
楚胜华%陈二涛%冯东福%马延斌%朱志安
楚勝華%陳二濤%馮東福%馬延斌%硃誌安
초성화%진이도%풍동복%마연빈%주지안
脑外伤%抑郁%动物模型%单胺类神经递质%形态学
腦外傷%抑鬱%動物模型%單胺類神經遞質%形態學
뇌외상%억욱%동물모형%단알류신경체질%형태학
Traumatic brain injury%Depression%Animal model%Monoamine neurotransmitter%Morphology
目的 探讨建立脑外伤后抑郁状态动物模型,并对此模型的建立和有效性做系统评价.方法 本实验在武汉大学医学院实验中心完成.选用48只雄性Wistar大鼠,随机(随机数字法)分为4组,每组12只.①假手术组:每笼6只饲养,正常饮食饮水；②模型组:以自由落体撞击法制作大鼠左侧额叶脑挫裂伤模型,每笼1只饲养,术后1周加以慢性应激和孤养方法,造成脑外伤后抑郁状态动物模型；③脑外伤组:以自由落体撞击法制作大鼠左侧额叶脑挫裂伤模型,每笼6只饲养,正常饮食饮水；④慢性不可预见性温和应激组:每笼1只饲养,采用慢性应激方法结合孤养法造成大鼠慢性应激抑郁模型.观察各组大鼠蔗糖水试验,自发性行为改变,用高效液相色谱荧光检测法测定各组大鼠脑内海马、前额叶、下丘脑和纹状体不同部位的多巴胺、5-羟色胺、去甲肾上腺素单胺类神经递质含量.采用SPSS 13.0统计软件分析结果,资料采用单因素方差分析或成组t检验,以P＜ 0.05为差异具有统计学意义.结果 造模后即予行为学测试,模型组大鼠蔗糖水饮用量逐渐减少,反映模型组大鼠兴趣和快感缺乏；旷野试验中模型组大鼠水平及垂直得分减少,反映模型组大鼠活动性减少,兴趣缺乏；模型组大鼠游到终点的时间延长,错误次数增加,反映模型组大鼠的学习和记忆能力下降；模型组大鼠海马、前额叶、下丘脑,和纹状体多巴胺、5-羟色胺、去甲肾上腺素含量下降.结论 在左侧额叶脑挫裂伤模型的基础上给予孤养和慢性不可预见的温和性应激,能建立较理想的脑外伤后抑郁状态动物模型.
目的 探討建立腦外傷後抑鬱狀態動物模型,併對此模型的建立和有效性做繫統評價.方法 本實驗在武漢大學醫學院實驗中心完成.選用48隻雄性Wistar大鼠,隨機(隨機數字法)分為4組,每組12隻.①假手術組:每籠6隻飼養,正常飲食飲水；②模型組:以自由落體撞擊法製作大鼠左側額葉腦挫裂傷模型,每籠1隻飼養,術後1週加以慢性應激和孤養方法,造成腦外傷後抑鬱狀態動物模型；③腦外傷組:以自由落體撞擊法製作大鼠左側額葉腦挫裂傷模型,每籠6隻飼養,正常飲食飲水；④慢性不可預見性溫和應激組:每籠1隻飼養,採用慢性應激方法結閤孤養法造成大鼠慢性應激抑鬱模型.觀察各組大鼠蔗糖水試驗,自髮性行為改變,用高效液相色譜熒光檢測法測定各組大鼠腦內海馬、前額葉、下丘腦和紋狀體不同部位的多巴胺、5-羥色胺、去甲腎上腺素單胺類神經遞質含量.採用SPSS 13.0統計軟件分析結果,資料採用單因素方差分析或成組t檢驗,以P＜ 0.05為差異具有統計學意義.結果 造模後即予行為學測試,模型組大鼠蔗糖水飲用量逐漸減少,反映模型組大鼠興趣和快感缺乏；曠野試驗中模型組大鼠水平及垂直得分減少,反映模型組大鼠活動性減少,興趣缺乏；模型組大鼠遊到終點的時間延長,錯誤次數增加,反映模型組大鼠的學習和記憶能力下降；模型組大鼠海馬、前額葉、下丘腦,和紋狀體多巴胺、5-羥色胺、去甲腎上腺素含量下降.結論 在左側額葉腦挫裂傷模型的基礎上給予孤養和慢性不可預見的溫和性應激,能建立較理想的腦外傷後抑鬱狀態動物模型.
목적 탐토건립뇌외상후억욱상태동물모형,병대차모형적건립화유효성주계통평개.방법 본실험재무한대학의학원실험중심완성.선용48지웅성Wistar대서,수궤(수궤수자법)분위4조,매조12지.①가수술조:매롱6지사양,정상음식음수；②모형조:이자유락체당격법제작대서좌측액협뇌좌렬상모형,매롱1지사양,술후1주가이만성응격화고양방법,조성뇌외상후억욱상태동물모형；③뇌외상조:이자유락체당격법제작대서좌측액협뇌좌렬상모형,매롱6지사양,정상음식음수；④만성불가예견성온화응격조:매롱1지사양,채용만성응격방법결합고양법조성대서만성응격억욱모형.관찰각조대서자당수시험,자발성행위개변,용고효액상색보형광검측법측정각조대서뇌내해마、전액협、하구뇌화문상체불동부위적다파알、5-간색알、거갑신상선소단알류신경체질함량.채용SPSS 13.0통계연건분석결과,자료채용단인소방차분석혹성조t검험,이P＜ 0.05위차이구유통계학의의.결과 조모후즉여행위학측시,모형조대서자당수음용량축점감소,반영모형조대서흥취화쾌감결핍；광야시험중모형조대서수평급수직득분감소,반영모형조대서활동성감소,흥취결핍；모형조대서유도종점적시간연장,착오차수증가,반영모형조대서적학습화기억능력하강；모형조대서해마、전액협、하구뇌,화문상체다파알、5-간색알、거갑신상선소함량하강.결론 재좌측액협뇌좌렬상모형적기출상급여고양화만성불가예견적온화성응격,능건립교이상적뇌외상후억욱상태동물모형.
Objective To discuss about the establishment of mental depression model by traumatic brain injury in rats,and to evaluate the validity of this model.Methods The study was carried out in the experiment center of Wuhan University.A total of 48 male adult Wistar rats were randomized ( random number) into four groups ( n =12,each):( 1 ) in sham operation group,six rats were fed normally in one cage; (2) in model group,the model of the left frontal lobe contusion was made up by hitting with free-fall method on the left cortex of the rat,and subsequently the each of model rats were separately fed in a cage and put them in a lonely environment with chronic stress one week after traumatic brain injury in order to induce them into models of mental depression; ( 3 ) in brain trauma group,the trauma model of the left frontal lobe contusion was set up by the same procedure as in model group,and six rats were fed together in one cage; (4) in CUMS group,each rat was fed normally in one cage in a lonely environment after brain trauma made and chronic mild chaotic stress unpredictable to rats was given to induce mental depression.The consumption of sucrose water and the change of animal behavior were observed and the high performance liquid phase electrochemical with fluorescence detector was used to detect the biogenic monoamine neurotransmitter content (dopamine,serotonin and norepinephrine) in the hippocampus,prefrontal cortex,hypothalamus and striatum area in each group.Experimental data were processed with single factor analysis or t test of variance by Statistical Program for Social Sciences Version 13.0 (SPSS13.0) software.P ＜0.05 was considered as statistically significant difference.Results After modeling,the rats of four groups were examined by behavioral tests.It was found that the amount of sugar-water consumption by the rats in the model group and CUMP group decreased,implying the absence of interest in eating and anhedonia of the rats in these groups.In open-field test,the rats in the model group,CUMP group and brain trauma group showed reduction in square crossing and rearing,implying the under-activity and absence of interest in activity.In the water maze test,it was found that the rats in the model group,CUMP group and brain trauma group needed much time to reach the end-point and made many errors,implying the lowering ability to learn and memorize.Levels of dopamine,serotonin and norepinephrine in the hippocampus,prefrontal cortex,hypothalamus and striatum area decreased in these rats with traumatized brain in three groups especially in model group and CUMP group.Conclusions It is a valid method for establishing the mental depression model in rats by traumatic brain injury with separately feeding in the lonely environment and given chronic mild chaotic stress unpredictable to rats on the setting of the left frontal lobe contusion.
