中国行为医学科学
中國行為醫學科學
중국행위의학과학
2008年
7期
594-596
,共3页
刘春喜%黄耀德%刘文超%李志宏
劉春喜%黃耀德%劉文超%李誌宏
류춘희%황요덕%류문초%리지굉
阿尔茨海默病%内嗅皮质%β淀粉样蛋白%学习记忆
阿爾茨海默病%內嗅皮質%β澱粉樣蛋白%學習記憶
아이자해묵병%내후피질%β정분양단백%학습기억
Alzheimer's disease%Entorhinal cortex%Amyloid-beta protein%Learning and memory ability
目的 观察β淀粉样蛋白(Aβ)的在体神经毒作用及其对大鼠学习记忆能力的影响.方法 将聚集态Aβ微量注射于大鼠双侧内嗅皮质(EC),对照组注射生理盐水(NS),术后第7,8天采用Y-迷宫进行行为学测试,测试后处死动物取材,分别采用尼氏染色、HE染色、刚果红染色观察EC的病理改变.结果 NS组大鼠获得记忆和再现记忆累积尝试次数分别是(19.17±5.58)次、(10.56±1.72)次,AB组大鼠获得记忆和再现记忆尝试次数分别是(31.13±6.98)次、(18.47±4.64)次,组间差异有显著性(P<0.01);Aβ组注射区刚果红染色阳性,并有Aβ沉积、神经元丢失、胶质细胞增生,受损面积明显大于NS组,受损面积与动物的学习记忆水平呈现出负相关性(r=-0.241,P<0.05).结论 双侧EC注射聚集态Aβ可以引起动物学习记忆障碍,注射区局部的病理改变与阿尔茨海默病(AD)典型的老年斑(SP)相似,说明聚集态Aβ具有明确的神经毒作用,EC内注射Aβ有望成为研究AD发病机理与Aβ相关药物筛选的动物模型之一.
目的 觀察β澱粉樣蛋白(Aβ)的在體神經毒作用及其對大鼠學習記憶能力的影響.方法 將聚集態Aβ微量註射于大鼠雙側內嗅皮質(EC),對照組註射生理鹽水(NS),術後第7,8天採用Y-迷宮進行行為學測試,測試後處死動物取材,分彆採用尼氏染色、HE染色、剛果紅染色觀察EC的病理改變.結果 NS組大鼠穫得記憶和再現記憶纍積嘗試次數分彆是(19.17±5.58)次、(10.56±1.72)次,AB組大鼠穫得記憶和再現記憶嘗試次數分彆是(31.13±6.98)次、(18.47±4.64)次,組間差異有顯著性(P<0.01);Aβ組註射區剛果紅染色暘性,併有Aβ沉積、神經元丟失、膠質細胞增生,受損麵積明顯大于NS組,受損麵積與動物的學習記憶水平呈現齣負相關性(r=-0.241,P<0.05).結論 雙側EC註射聚集態Aβ可以引起動物學習記憶障礙,註射區跼部的病理改變與阿爾茨海默病(AD)典型的老年斑(SP)相似,說明聚集態Aβ具有明確的神經毒作用,EC內註射Aβ有望成為研究AD髮病機理與Aβ相關藥物篩選的動物模型之一.
목적 관찰β정분양단백(Aβ)적재체신경독작용급기대대서학습기억능력적영향.방법 장취집태Aβ미량주사우대서쌍측내후피질(EC),대조조주사생리염수(NS),술후제7,8천채용Y-미궁진행행위학측시,측시후처사동물취재,분별채용니씨염색、HE염색、강과홍염색관찰EC적병리개변.결과 NS조대서획득기억화재현기억루적상시차수분별시(19.17±5.58)차、(10.56±1.72)차,AB조대서획득기억화재현기억상시차수분별시(31.13±6.98)차、(18.47±4.64)차,조간차이유현저성(P<0.01);Aβ조주사구강과홍염색양성,병유Aβ침적、신경원주실、효질세포증생,수손면적명현대우NS조,수손면적여동물적학습기억수평정현출부상관성(r=-0.241,P<0.05).결론 쌍측EC주사취집태Aβ가이인기동물학습기억장애,주사구국부적병리개변여아이자해묵병(AD)전형적노년반(SP)상사,설명취집태Aβ구유명학적신경독작용,EC내주사Aβ유망성위연구AD발병궤리여Aβ상관약물사선적동물모형지일.
Objective To investigate the neurotoxicity of β-amyloid protein (Aβ) in vivo and its effects on rats' learning and memory ability. Methods The aggregated Aβ(25-35) was injected bilaterally into entorbi- hal cortex(EC) of rats. Control rats were injected with saline water. All rats were subjected to Y-maze task at 7 d and 8d after operation. After behavioral testing, the rats were sacrificed. The neurpathological changes of EC were examined by Nissl staining, HE staining and Congo Red staining. Results The cumulative number of electric shocks in acquisition test and retention test of the saline group was 19.17±5.58 and 10.56±1.72 31 respectively. The corresponding number in Aβ-treated rats was 31.13±6.98 and 18.47±4.64, and significantly increased compared with control group (P<0.05). A Congo Red-positive deposit of aggregated material was found at Aβ injection site accompanied by neurons loss and gliacyte reaction. The damaged area of Aβ-treated rats was significandy greater than that of control rats and was negatively correlated with the learning ability (r=-0.241, P < 0.05). Conclusion Bilateral injection of aggregated Aβ into EC induces behavioral deficits and amyloid pathology similar to those observed in early phases of AD. These results show definite neurotoxicity of Aβ in vivo. Therefore, injection of Aβ into EC constitutes a promising animal model for investigating selective aspects of AD and for screening drug candidates designed against Aβ pathology.
