中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2012年
9期
743-748
,共6页
鼻窦炎%疾病模型,动物%嗅觉障碍%嗅黏膜%嗅觉受体神经元%神经胶质%荧光抗体技术
鼻竇炎%疾病模型,動物%嗅覺障礙%嗅黏膜%嗅覺受體神經元%神經膠質%熒光抗體技術
비두염%질병모형,동물%후각장애%후점막%후각수체신경원%신경효질%형광항체기술
Sinusitis%Disease models,animal%Olfaction disorders%Olfactory mucosa%Olfactory receptor neurons%Neuroglia%Fluorescent antibody technique
目的 观察大鼠急性鼻-鼻窦炎致嗅觉障碍模型中嗅黏膜微结构的变化情况,为进一步探索鼻-鼻窦炎对嗅觉功能影响的可能机制提供实验基础.方法 健康SD大鼠100只采用随机数字表法分为实验组(80只)和对照组(20只).实验组使用肺炎链球菌建立大鼠急性鼻-鼻窦炎动物模型,运用嗅觉功能行为学(食物小球埋藏法)检测实验模型的嗅觉功能,采用SPSS 13.0统计软件对该数据进行DunnettT3检验.分别于接种细菌后第1(A组)、2(B组)、3(C组)、4周(D组)各处死20只实验组大鼠;对照组(E组)大鼠未接种细菌且均于第1周处死.取包含鼻顶部嗅黏膜的鼻腔鼻窦黏膜组织,制作冰冻切片、采用免疫荧光技术观察嗅黏膜中成熟嗅感觉神经元和嗅鞘细胞的形态变化.结果 嗅觉功能行为学检测实验结果显示,A、B、C、D组大鼠找到食物小球的时间[分别为(402.9±9.3)、(453.7±7.3)、(351.9±8.9)、(278.7±8.1)s]均较对照组[(178.3±6.6)s]长,差异有统计学意义(P值均＜0.01).实验组嗅黏膜较对照组均有不同程度的成熟嗅感觉神经元数量减少及黏膜上皮层变薄倾向,尤以造模后第2周最为严重,至第4周时最轻.实验组大鼠嗅黏膜固有层嗅鞘细胞在出现短时间的减少后,自第2周起先于嗅感觉神经元出现数量增加.至第4周,固有层的嗅鞘细胞基本恢复正常.所有实验组动物嗅上皮均有少量嗅鞘细胞生长.结论 发生急性鼻-鼻窦炎时,嗅黏膜中成熟嗅感觉神经元和嗅鞘细胞均出现数量减少.但嗅鞘细胞先于嗅感觉神经元增多,并且嗅上皮亦出现少量嗅鞘细胞生长.
目的 觀察大鼠急性鼻-鼻竇炎緻嗅覺障礙模型中嗅黏膜微結構的變化情況,為進一步探索鼻-鼻竇炎對嗅覺功能影響的可能機製提供實驗基礎.方法 健康SD大鼠100隻採用隨機數字錶法分為實驗組(80隻)和對照組(20隻).實驗組使用肺炎鏈毬菌建立大鼠急性鼻-鼻竇炎動物模型,運用嗅覺功能行為學(食物小毬埋藏法)檢測實驗模型的嗅覺功能,採用SPSS 13.0統計軟件對該數據進行DunnettT3檢驗.分彆于接種細菌後第1(A組)、2(B組)、3(C組)、4週(D組)各處死20隻實驗組大鼠;對照組(E組)大鼠未接種細菌且均于第1週處死.取包含鼻頂部嗅黏膜的鼻腔鼻竇黏膜組織,製作冰凍切片、採用免疫熒光技術觀察嗅黏膜中成熟嗅感覺神經元和嗅鞘細胞的形態變化.結果 嗅覺功能行為學檢測實驗結果顯示,A、B、C、D組大鼠找到食物小毬的時間[分彆為(402.9±9.3)、(453.7±7.3)、(351.9±8.9)、(278.7±8.1)s]均較對照組[(178.3±6.6)s]長,差異有統計學意義(P值均＜0.01).實驗組嗅黏膜較對照組均有不同程度的成熟嗅感覺神經元數量減少及黏膜上皮層變薄傾嚮,尤以造模後第2週最為嚴重,至第4週時最輕.實驗組大鼠嗅黏膜固有層嗅鞘細胞在齣現短時間的減少後,自第2週起先于嗅感覺神經元齣現數量增加.至第4週,固有層的嗅鞘細胞基本恢複正常.所有實驗組動物嗅上皮均有少量嗅鞘細胞生長.結論 髮生急性鼻-鼻竇炎時,嗅黏膜中成熟嗅感覺神經元和嗅鞘細胞均齣現數量減少.但嗅鞘細胞先于嗅感覺神經元增多,併且嗅上皮亦齣現少量嗅鞘細胞生長.
목적 관찰대서급성비-비두염치후각장애모형중후점막미결구적변화정황,위진일보탐색비-비두염대후각공능영향적가능궤제제공실험기출.방법 건강SD대서100지채용수궤수자표법분위실험조(80지)화대조조(20지).실험조사용폐염련구균건립대서급성비-비두염동물모형,운용후각공능행위학(식물소구매장법)검측실험모형적후각공능,채용SPSS 13.0통계연건대해수거진행DunnettT3검험.분별우접충세균후제1(A조)、2(B조)、3(C조)、4주(D조)각처사20지실험조대서;대조조(E조)대서미접충세균차균우제1주처사.취포함비정부후점막적비강비두점막조직,제작빙동절편、채용면역형광기술관찰후점막중성숙후감각신경원화후초세포적형태변화.결과 후각공능행위학검측실험결과현시,A、B、C、D조대서조도식물소구적시간[분별위(402.9±9.3)、(453.7±7.3)、(351.9±8.9)、(278.7±8.1)s]균교대조조[(178.3±6.6)s]장,차이유통계학의의(P치균＜0.01).실험조후점막교대조조균유불동정도적성숙후감각신경원수량감소급점막상피층변박경향,우이조모후제2주최위엄중,지제4주시최경.실험조대서후점막고유층후초세포재출현단시간적감소후,자제2주기선우후감각신경원출현수량증가.지제4주,고유층적후초세포기본회복정상.소유실험조동물후상피균유소량후초세포생장.결론 발생급성비-비두염시,후점막중성숙후감각신경원화후초세포균출현수량감소.단후초세포선우후감각신경원증다,병차후상피역출현소량후초세포생장.
Objective To observe the microstructural changes of olfactory mucosa in rat model with acute rhinosinusitis leading to olfactory dysfunction,and to provide foundation for further exploration of corresponding mechanism.Methods On the basis of prior successfully established rat model of acute rhinosinusitis through inoculation with Streptococcus pneumoniae and with the help of merocel strips,one hundred healthy SD rats were randomly divided into experimental group (80) and control group (20).After inoculation,every 20 rats in the experimental groups were sacrificed in first week,second week,third week and fourth week respectively; and all rats in the control group were sacrificed in first week after the inoculation.Before the rats were sacrificed,the method called “buffed food pellet test,BFPT” was adopted,which was advanced by professor Nathan,to measure the rats’ olfaction,and the time of every rat spending in finding out the food pellet was recorded and analyzed.BFPT showed that the rats in experimental group spent (402.9±9.3),(453.7 ±7.3),(351.9±8.9),(278.7 ±8.1) s respectively in searching the food pellet,which were more than the rats in the control group[ ( 178.3 ±6.6) s].Then the olfactory mueosa was collected under anatomic microscope from all the rats to make frozen section and detect the changes of mature olfactory receptor neurons (ORN) and olfactory ensheathing cells (OEC) by immunofluorescence technique.Results The reduction of ORN in various degrees could be detected in the tissue samples of olfactory mucosa among all the rats in experimental group,with a tendency to become thinner in the thickness of epithelial lamina during the inflammation developing course.This kind of pathology was most marked in the second week and it gradually developed into the stage showing the lesion being the feeblest in the forth week following the beginning of modeling.Although the number of olfactory ensheathing cells appeared reduction in the first week following the beginning of modeling as well,it came to increase from the second week before olfactory receptor neurons and almost completely recovered to normal in the fourth week.In addition,some olfactory ensheathing cells could be detected in the tissue samples of olfactory mucosa among all the rats in experimental group.Conclusions Both mature olfactory sensory neurons and olfactory ensheathing cells appeared to reduction when sinonasal mucosa taken place acute rhinosinusitis.But the number of olfactory ensheathing cells increased faster than olfactory sensory neurons.In addition,some olfactory ensheathing cells could be detected in the olfactory epithelium.
