中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
2期
99-101
,共3页
哈达%孔迎辉%魏烨平%郑淑云
哈達%孔迎輝%魏燁平%鄭淑雲
합체%공영휘%위엽평%정숙운
Bowen病%乳头瘤病毒%聚合酶链反应%敏感性和特异性
Bowen病%乳頭瘤病毒%聚閤酶鏈反應%敏感性和特異性
Bowen병%유두류병독%취합매련반응%민감성화특이성
Bowen's disease%Human papillomavirus%Polymerase chain reaction%Sensitivity and specificity
目的 建立一种巢式PCR方法,探讨HPV DNA在非生殖器部位Bowen病中的检出率.方法 巢式PCR方法,用5对不同引物包括CN1FR、CN2FR、CN3FR、CN4FR以及CN5FR进行扩增.结果 通过ClustalX软件,将所设计的每对引物与已知HPV亚型的碱基序列逐一相比较,得知改良设计的引物可以使69种HPV亚型得到扩增,包括黏膜型HPV、皮肤型HPV以及疣状表皮发育不良相关性HPV;PCR反应体系的敏感度在10-2～10-3拷贝之间.41例非生殖器部位Bowen病的组织标本中,5例HPV DNA阳性,其中高危黏膜型3例(2例HPV16,1例HPV33),皮肤型2例(HPV27和HPV76各1例).结论 改良的巢式PCR方法具有较高的敏感性、特异性,部分非生殖器部位Bowen病发病与黏膜高危型HPV感染相关.
目的 建立一種巢式PCR方法,探討HPV DNA在非生殖器部位Bowen病中的檢齣率.方法 巢式PCR方法,用5對不同引物包括CN1FR、CN2FR、CN3FR、CN4FR以及CN5FR進行擴增.結果 通過ClustalX軟件,將所設計的每對引物與已知HPV亞型的堿基序列逐一相比較,得知改良設計的引物可以使69種HPV亞型得到擴增,包括黏膜型HPV、皮膚型HPV以及疣狀錶皮髮育不良相關性HPV;PCR反應體繫的敏感度在10-2～10-3拷貝之間.41例非生殖器部位Bowen病的組織標本中,5例HPV DNA暘性,其中高危黏膜型3例(2例HPV16,1例HPV33),皮膚型2例(HPV27和HPV76各1例).結論 改良的巢式PCR方法具有較高的敏感性、特異性,部分非生殖器部位Bowen病髮病與黏膜高危型HPV感染相關.
목적 건립일충소식PCR방법,탐토HPV DNA재비생식기부위Bowen병중적검출솔.방법 소식PCR방법,용5대불동인물포괄CN1FR、CN2FR、CN3FR、CN4FR이급CN5FR진행확증.결과 통과ClustalX연건,장소설계적매대인물여이지HPV아형적감기서렬축일상비교,득지개량설계적인물가이사69충HPV아형득도확증,포괄점막형HPV、피부형HPV이급우상표피발육불량상관성HPV;PCR반응체계적민감도재10-2～10-3고패지간.41례비생식기부위Bowen병적조직표본중,5례HPV DNA양성,기중고위점막형3례(2례HPV16,1례HPV33),피부형2례(HPV27화HPV76각1례).결론 개량적소식PCR방법구유교고적민감성、특이성,부분비생식기부위Bowen병발병여점막고위형HPV감염상관.
Objective To develop a new nested PCR to detect human papillomavirus (HPV) DNA in lesions of extragenital Bowen's disease.Methods DNA was extracted from the lesions of 41 patients with extragenital Bowen's disease and suhjected to the amplification of HPV by a nested PCR.Five primers,including CN1FR,CN2FR,CN3FR,CN4FR and CN5FR,were designed and used in the second round PCR.Results Based on the ClustalX analysis,69 HPV subtypes,including mucosal types,cutaneous types and epidermodysplasia verruciformis-associated types,could be amplified by using the 5 designed primers.The detection limit varied from 10-3 to 10-2 copies of HPV DNA for this PCR.Of the 41 lesional specimens,5 were positive for HPV DNA,including 3 cases of high-risk HPV types (2 cases of HPV 16,1 case of HPV 33) and 2 cases of cutaneous HPV types (1 case of HPV 27 and 1 case of HPV 76).Conclusions The improved nested PCR is highly sensitive and specific for the detection of HPV DNA in lesions of extragenital Bowen's disease.The development of extragenital Bowen's disease may be associated with the infection with high-risk mucosal HPV types in some patients.
