中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2011年
2期
144-147
,共4页
叶明%周岱%周幽心%张世明%黄煜伦%徐峰
葉明%週岱%週幽心%張世明%黃煜倫%徐峰
협명%주대%주유심%장세명%황욱륜%서봉
神经胶质瘤%细胞凋亡%Survivin%小干扰RNA
神經膠質瘤%細胞凋亡%Survivin%小榦擾RNA
신경효질류%세포조망%Survivin%소간우RNA
Glioma%Apoptosis%Survivin%Small interfering RNA
目的 探讨siRNA敲除Survivin基因的可行性,并观察其对人脑胶质瘤细胞凋亡的影响.方法 采用人工合成的小干扰RNA(siRNA)体外阻抑人脑胶质瘤细胞T98和SF767 Survivin基因的表达,即时定量PCR方法检测Survivin mRNA表达水平,流式细胞仪检测细胞凋亡率.结果 Survivin siRNA转染后24 h,T98、SF767细胞中Survivin mRNA表达水平同阴性对照相比下调分别达92.6%、89.5%,48 h后表达量则下降了 80.1%、67.6%.T98细胞在干扰后24、48 h平均凋亡率分别达50.2%、38.4%,SF767分别达40.1%、25.6%,同阴性对照相比差异有统计学意义(P＜0.01).结论 针对Survivin的siRNA Oligo能有效抑制Survivin基因的表达,并诱导细胞凋亡,为RNA干扰技术用于胶质瘤的临床治疗打下了基础.
目的 探討siRNA敲除Survivin基因的可行性,併觀察其對人腦膠質瘤細胞凋亡的影響.方法 採用人工閤成的小榦擾RNA(siRNA)體外阻抑人腦膠質瘤細胞T98和SF767 Survivin基因的錶達,即時定量PCR方法檢測Survivin mRNA錶達水平,流式細胞儀檢測細胞凋亡率.結果 Survivin siRNA轉染後24 h,T98、SF767細胞中Survivin mRNA錶達水平同陰性對照相比下調分彆達92.6%、89.5%,48 h後錶達量則下降瞭 80.1%、67.6%.T98細胞在榦擾後24、48 h平均凋亡率分彆達50.2%、38.4%,SF767分彆達40.1%、25.6%,同陰性對照相比差異有統計學意義(P＜0.01).結論 針對Survivin的siRNA Oligo能有效抑製Survivin基因的錶達,併誘導細胞凋亡,為RNA榦擾技術用于膠質瘤的臨床治療打下瞭基礎.
목적 탐토siRNA고제Survivin기인적가행성,병관찰기대인뇌효질류세포조망적영향.방법 채용인공합성적소간우RNA(siRNA)체외조억인뇌효질류세포T98화SF767 Survivin기인적표체,즉시정량PCR방법검측Survivin mRNA표체수평,류식세포의검측세포조망솔.결과 Survivin siRNA전염후24 h,T98、SF767세포중Survivin mRNA표체수평동음성대조상비하조분별체92.6%、89.5%,48 h후표체량칙하강료 80.1%、67.6%.T98세포재간우후24、48 h평균조망솔분별체50.2%、38.4%,SF767분별체40.1%、25.6%,동음성대조상비차이유통계학의의(P＜0.01).결론 침대Survivin적siRNA Oligo능유효억제Survivin기인적표체,병유도세포조망,위RNA간우기술용우효질류적림상치료타하료기출.
Objective This study was to investigate the feasibility ofknockdown of Survivin gene with small interfering RNA and to observe the apoptosis in gliomas which was influenced by siRNA.Methods Survivin specific siRNA oligonucleotides were designed and synthesized artificially.This siRNA were transfected into human glioma cells T98 and SF767 to inhibit the expression of Survivin RNA in vitro.The mRNA level of Survivin was detected by real- time quantitative polymerase chain reaction (PCR) and the apoptosis of cells was assayed by flow cytometry (FCM).Methods The expression of Survivin mRNA in siRNA- transfected samples decreased by 92.6% and 89.5% in T98,SF767 cells respectively in 24 hours after transfected with siRNA oligos.The expression amount was declined by 80.1% and 67.6% in 48 hours after RNAi.The apoptosis rate of T98 cells was 50.2% in 24 hours after interfere,and was 38.4% in 48 hours.The apoptosis rate of SF757 cells were 40.1% and 25.6% respectively.There was a significant difference between the RNAi cells and negative controls(P ＜0.01 ).ConclusionThe specific siRNA oligo which was aim to Survivin could knockdown the expression of Survivin mRNA,and induce apoptosis in human glioma cells.The experiment had made the foundation of the clinical RNA interfering technique.
