CAJ | 학술논문

目的探讨甘露糖结合蛋白(mannose binding protein,MBP)基因变异与慢性及重型乙型肝炎的相关性.方法采用基冈扩增和DNA测序方法检测了64名健康人和52例慢性乙肝及62例重型乙肝患者MBP基凶第1外显子的DNA序列,然后与GenBank中参照序列进行比较分析.结果 3组人群中MBP基因仅存在密码子54突变,而无密码子52、57的突变.健康人、慢性乙肝和重型乙肝组密码子54突变率分别为14.1%(9/64)、15.4%(8/52)和35.5%(22/62),前两组间比较差异无统计学意义(P＞0.05),而重型乙肝组密码子54突变率则分别显著高于前两组(X2=7,79,P＜0.01;x2=5.89,P＜0.05).结论该地区乙肝人群MBP基因仅存在密码子54突变,突变与乙肝慢性化无明显关系,而与重型肝炎的发生有显著相关性.故监测MBP基因突变可作为重型肝炎判断预后的一个可行性指标.
목적 탐토감로당결합단백(mannose binding protein,MBP)기인변이여만성급중형을형간염적상관성.방법 채용기강확증화DNA측서방법검측료64명건강인화52례만성을간급62례중형을간환자MBP기흉제1외현자적DNA서렬,연후여GenBank중삼조서렬진행비교분석.결과 3조인군중MBP기인부존재밀마자54돌변,이무밀마자52、57적돌변.건강인、만성을간화중형을간조밀마자54돌변솔분별위14.1%(9/64)、15.4%(8/52)화35.5%(22/62),전량조간비교차이무통계학의의(P＞0.05),이중형을간조밀마자54돌변솔칙분별현저고우전량조(X2=7,79,P＜0.01;x2=5.89,P＜0.05).결론 해지구을간인군MBP기인부존재밀마자54돌변,돌변여을간만성화무명현관계,이여중형간염적발생유현저상관성.고감측MBP기인돌변가작위중형간염판단예후적일개가행성지표.
Objective To investigate the relationship between the gene mutations of inannose binding protein (MBP)and the pmgression of hepatitis B.Methods The MBP gene mutations in 52 patients with chronic hepatitis B and 62 paticnIs with severe hepatitis B and 64 HBsAg-negative healthy controls were investigated.The mutations in MBP gene were analyzed by polyInerase chain reaction(PCR)and direct DNA sequencing. Results A mutation of MBP gene codon 54 was found.The mutation frequency in the group of severe hepatitis B(35.5%,22/62)was higher than those in the chronic hepatitis B group (15.4%,8/52) and the HBsAg-negative healthy controls(14.1%,9/64),respeefively,and their difference was significant(x2=7.79,P<0.01;x2=5.89,P<0.05).The difference between the chronic hepatitis B group and the HBsAg-negative healthy control group was not significant(P>0.05).Conclucion There is onlv mut4tion in codon 54 of the MBP gene in patients with hepatitis B infection in the area analyzed.Codon 54 mutation of MBP gene is not related to the persistence of hepatitis B,but it was associated with the progression of hepatitis B infection.