CAJ | 학술논문

目的探讨精索静脉曲张大鼠干细胞因子(SCF)的变化和意义.方法将清洁级雄性SD大鼠20只喂养1周后,随机取10只应用左肾静脉部分结扎法进行造模,设为模型组；另外10只行左肾静脉分离后不结扎,设为假手术组.两组均于60d后处死大鼠,检测血清SCF、睾丸组织SCF及附睾精子的密度和活率.结果模型组睾丸组织SCF为(654.6±30.5)ng/L,与假手术组(382.8±25.6) ng/L比较明显升高(P＜0.05)；两组血清SCF比较差异无统计学意义(P＞0.05).模型组大鼠附睾精子密度为(2.36±1.58),与假手术组(4.07±1.40)比较明显降低(P＜0.05),两组大鼠精子活率比较差异无统计学意义(P＞0.05).结论检测睾丸组织SCF有助于判断生精细胞损伤.
목적 탐토정색정맥곡장대서간세포인자(SCF)적변화화의의.방법 장청길급웅성SD대서20지위양1주후,수궤취10지응용좌신정맥부분결찰법진행조모,설위모형조；령외10지행좌신정맥분리후불결찰,설위가수술조.량조균우60d후처사대서,검측혈청SCF、고환조직SCF급부고정자적밀도화활솔.결과 모형조고환조직SCF위(654.6±30.5)ng/L,여가수술조(382.8±25.6) ng/L비교명현승고(P＜0.05)；량조혈청SCF비교차이무통계학의의(P＞0.05).모형조대서부고정자밀도위(2.36±1.58),여가수술조(4.07±1.40)비교명현강저(P＜0.05),량조대서정자활솔비교차이무통계학의의(P＞0.05).결론 검측고환조직SCF유조우판단생정세포손상.
Objective To investigate the change and significance of stem cell factor (SCF) of the rats with varicocele.Methods To fed twenty cleaning wistar male SD rats for a week,10 rats were randomly selected and which was inducted varicocele group by partial ligation of the left renal vein.Another 10 rats set up for the sham group.The rats of both groups were killed after 60 days,take the postmortem testis and epididymis.We detected the SCF in serum and testicular tissue and detected the density and survival rate of epididymal sperm.Results Compared with the model group,the sham group on sperm density (4.07 ± 1.40) was significantly higher (P ＜ 0.05 ),epididymal sperm motility were not significantly different ( P ＞ 0.05 ).Sham group testes SCF (654.60:±:30.50 ) ng/L significantly decreased ( P ＜ 0.05 )than the model group.SCF in serum showed no significant difference between the groups (P ＞ 0.05 ).Conclusion SCF in testis might serve as an indicator of spermatogenic cells injury.