中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2011年
10期
764-769
,共6页
耿一婷%仇金荣%王蓉%苏云涛%束永前%殷咏梅
耿一婷%仇金榮%王蓉%囌雲濤%束永前%慇詠梅
경일정%구금영%왕용%소운도%속영전%은영매
胃肿瘤%人表皮生长因子受体2%瘦素%瘦素受体%血管内皮生长因子%预后
胃腫瘤%人錶皮生長因子受體2%瘦素%瘦素受體%血管內皮生長因子%預後
위종류%인표피생장인자수체2%수소%수소수체%혈관내피생장인자%예후
Stomach neoplasms%Human epidermal growth factor receptor-2%Leptin%Leptin receptor%Vascular endothelial growth factor%Prognosis
目的 探讨胃癌组织中人表皮生长因子受体2(HER-2)、瘦素与瘦素受体的表达及其与临床病理特征和预后的关系,明确HER-2在胃癌原发灶与转移淋巴结的表达的一致性及胃癌组织中HER-2、瘦素和血管内皮生长因子(VEGF)表达水平的关系.方法 选取110例胃癌手术切除组织和相应转移淋巴结作为研究对象,以96例正常胃黏膜组织作为对照,应用免疫组织化学法检测HER-2、瘦素、瘦素受体b亚型(OB-Rb)和VEGF蛋白的表达水平.结果 HER-2、瘦素和OB-Rb在胃癌组织的表达阳性率分别为19.1％、49.1％和60.9％,显著高于正常胃黏膜组织(分别为8.0％、34.0％和46.0％,均P＜0.05).HER-2在胃癌原发灶与淋巴结转移灶中的表达状态差异无统计学意义(P=0.607),一致性呈中等水平(Kappa=0.581).在胃癌组织中,HER-2和瘦素的表达呈正相关(r=0.217,P=0.023),HER-2和瘦素与VEGF的表达也呈正相关(r=0.263,P=0.005；r=0.200,P=0.036).HER-2和瘦素的表达与肿瘤浸润深度、淋巴结及远处转移、TNM分期等因素有关,而OB-Rb的表达与各临床病理学因素皆无关.Cox回归多因素分析结果显示,肿瘤低分化、体积大、转移淋巴结比例高、分期晚、术后不接受化疗和HER-2高表达为胃癌患者的独立不良预后因素.结论 HER-2在胃癌原发灶与转移淋巴结中的表达状态基本一致.HER-2与瘦素在胃癌的侵袭进展、血管形成过程中可能起重要作用,HER-2高表达是胃癌患者的不良预后因素.
目的 探討胃癌組織中人錶皮生長因子受體2(HER-2)、瘦素與瘦素受體的錶達及其與臨床病理特徵和預後的關繫,明確HER-2在胃癌原髮竈與轉移淋巴結的錶達的一緻性及胃癌組織中HER-2、瘦素和血管內皮生長因子(VEGF)錶達水平的關繫.方法 選取110例胃癌手術切除組織和相應轉移淋巴結作為研究對象,以96例正常胃黏膜組織作為對照,應用免疫組織化學法檢測HER-2、瘦素、瘦素受體b亞型(OB-Rb)和VEGF蛋白的錶達水平.結果 HER-2、瘦素和OB-Rb在胃癌組織的錶達暘性率分彆為19.1％、49.1％和60.9％,顯著高于正常胃黏膜組織(分彆為8.0％、34.0％和46.0％,均P＜0.05).HER-2在胃癌原髮竈與淋巴結轉移竈中的錶達狀態差異無統計學意義(P=0.607),一緻性呈中等水平(Kappa=0.581).在胃癌組織中,HER-2和瘦素的錶達呈正相關(r=0.217,P=0.023),HER-2和瘦素與VEGF的錶達也呈正相關(r=0.263,P=0.005；r=0.200,P=0.036).HER-2和瘦素的錶達與腫瘤浸潤深度、淋巴結及遠處轉移、TNM分期等因素有關,而OB-Rb的錶達與各臨床病理學因素皆無關.Cox迴歸多因素分析結果顯示,腫瘤低分化、體積大、轉移淋巴結比例高、分期晚、術後不接受化療和HER-2高錶達為胃癌患者的獨立不良預後因素.結論 HER-2在胃癌原髮竈與轉移淋巴結中的錶達狀態基本一緻.HER-2與瘦素在胃癌的侵襲進展、血管形成過程中可能起重要作用,HER-2高錶達是胃癌患者的不良預後因素.
목적 탐토위암조직중인표피생장인자수체2(HER-2)、수소여수소수체적표체급기여림상병리특정화예후적관계,명학HER-2재위암원발조여전이림파결적표체적일치성급위암조직중HER-2、수소화혈관내피생장인자(VEGF)표체수평적관계.방법 선취110례위암수술절제조직화상응전이림파결작위연구대상,이96례정상위점막조직작위대조,응용면역조직화학법검측HER-2、수소、수소수체b아형(OB-Rb)화VEGF단백적표체수평.결과 HER-2、수소화OB-Rb재위암조직적표체양성솔분별위19.1％、49.1％화60.9％,현저고우정상위점막조직(분별위8.0％、34.0％화46.0％,균P＜0.05).HER-2재위암원발조여림파결전이조중적표체상태차이무통계학의의(P=0.607),일치성정중등수평(Kappa=0.581).재위암조직중,HER-2화수소적표체정정상관(r=0.217,P=0.023),HER-2화수소여VEGF적표체야정정상관(r=0.263,P=0.005；r=0.200,P=0.036).HER-2화수소적표체여종류침윤심도、림파결급원처전이、TNM분기등인소유관,이OB-Rb적표체여각림상병이학인소개무관.Cox회귀다인소분석결과현시,종류저분화、체적대、전이림파결비례고、분기만、술후불접수화료화HER-2고표체위위암환자적독립불량예후인소.결론 HER-2재위암원발조여전이림파결중적표체상태기본일치.HER-2여수소재위암적침습진전、혈관형성과정중가능기중요작용,HER-2고표체시위암환자적불량예후인소.
Objective To assess the expression of HER-2 and leptin in gastric cancer and evaluate their relationship with VEGF expression and clinicopathological features,and their prognostic value for gastric cancer patients.Methods One hundred and ten gastric cancer specimens and the corresponding metastatic lymph nodes were detected for HER-2 by immunohistochemistry(IHC).All primary cancer tissues were detected for leptin,OB-Rb and VEGF.Ninty-six specimens of normal gastric mucosa served as the control.Results The expression level of HER-2,leptin and OB-Rb in gastric cancer tissues were significantly higher than those in normal tissues(19.1％ vs.8.0％,49.1％ vs.34.0％,and 60.9％ vs.46.0％,P ＜ 0.05).HER-2 overexpression was moderately homogenous in primary gastric cancer and matastatic lymph nodes(P=0.607,Kappa=0.581).There was a correlation between the expression of HER-2 and leptin,both of which were significantly correlated with tumor invasion depth,metastatic lymph nodes ratio(NR),distal metastasis,TNM stage and VEGF expression.However,there was no significant correlation between OB-Rb expression and the clinicopathological features evaluated.Cox regression multivariate analysis showed that tumor size,histological grade,NR,stage,chemotherapy and HER-2 expression were independent prognostic factors.Conclusions HER-2 is stably expressed in primary gastric cancer and metastatic lymph nodes.HER-2 and leptin play an important role in the progression and angiogenesis of gastric cancer.High expression of HER-2 is a prognostic factor for poor outcome.
