肿瘤
腫瘤
종류
TUMOR
2010年
1期
62-67
,共6页
范志松%张慧%刘巍%范忠林%王玉栋%陈勇%耿翠芝%李海平%刘运江%刘月平%王小玲
範誌鬆%張慧%劉巍%範忠林%王玉棟%陳勇%耿翠芝%李海平%劉運江%劉月平%王小玲
범지송%장혜%류외%범충림%왕옥동%진용%경취지%리해평%류운강%류월평%왕소령
乳腺肿瘤%硫酸酯酶类%雌激素类%免疫组织化学%类固醇硫酸酯酶
乳腺腫瘤%硫痠酯酶類%雌激素類%免疫組織化學%類固醇硫痠酯酶
유선종류%류산지매류%자격소류%면역조직화학%류고순류산지매
Breast neoplasms%Sulfatases%Estrogens%Immunohistochemistry%Steroid sulfatase
目的:探讨类固醇硫酸酯酶(steroid sulfatase,STS)蛋白及其mRNA在乳腺癌组织及相对应的正常乳腺组织中的表达,以及与临床病理特征之间的关系.方法:分别应用免疫组织化学SP法和RT-PCR法检测40例乳腺癌患者乳腺癌组织及其对应的癌旁正常乳腺组织中STS蛋白和mRNA的表达水平,并进行比较,同时分析STS蛋白表达与临床病理特征之间的关系.结果: STS蛋白主要表达于乳腺癌细胞和正常乳腺组织上皮细胞的细胞质内,有3例患者乳腺癌细胞的细胞核中可见STS蛋白的表达,乳腺间质组织未见STS蛋白表达.乳腺癌组织及正常乳腺组织中STS蛋白的表达水平与其mRNA的表达水平均呈正相关.乳腺癌组织中STS蛋白的阳性表达率(70.0%)明显高于其对应的正常乳腺组织(42.5%),差异有统计学意义(P=0.013).分层分析显示,绝经前、淋巴结转移和病理分期较晚的乳腺癌患者,其乳腺癌组织中STS蛋白的阳性表达率明显高于其对应的正常乳腺组织(P<0.05).结论:乳腺癌组织可能通过高表达STS以促进自身合成雌激素,提高肿瘤局部的雌激素水平,从而促进肿瘤的生长和转移.同时,随着肿瘤的生长,肿瘤局部合成的雌激素在肿瘤生长中的作用可能越来越重要.
目的:探討類固醇硫痠酯酶(steroid sulfatase,STS)蛋白及其mRNA在乳腺癌組織及相對應的正常乳腺組織中的錶達,以及與臨床病理特徵之間的關繫.方法:分彆應用免疫組織化學SP法和RT-PCR法檢測40例乳腺癌患者乳腺癌組織及其對應的癌徬正常乳腺組織中STS蛋白和mRNA的錶達水平,併進行比較,同時分析STS蛋白錶達與臨床病理特徵之間的關繫.結果: STS蛋白主要錶達于乳腺癌細胞和正常乳腺組織上皮細胞的細胞質內,有3例患者乳腺癌細胞的細胞覈中可見STS蛋白的錶達,乳腺間質組織未見STS蛋白錶達.乳腺癌組織及正常乳腺組織中STS蛋白的錶達水平與其mRNA的錶達水平均呈正相關.乳腺癌組織中STS蛋白的暘性錶達率(70.0%)明顯高于其對應的正常乳腺組織(42.5%),差異有統計學意義(P=0.013).分層分析顯示,絕經前、淋巴結轉移和病理分期較晚的乳腺癌患者,其乳腺癌組織中STS蛋白的暘性錶達率明顯高于其對應的正常乳腺組織(P<0.05).結論:乳腺癌組織可能通過高錶達STS以促進自身閤成雌激素,提高腫瘤跼部的雌激素水平,從而促進腫瘤的生長和轉移.同時,隨著腫瘤的生長,腫瘤跼部閤成的雌激素在腫瘤生長中的作用可能越來越重要.
목적:탐토류고순류산지매(steroid sulfatase,STS)단백급기mRNA재유선암조직급상대응적정상유선조직중적표체,이급여림상병리특정지간적관계.방법:분별응용면역조직화학SP법화RT-PCR법검측40례유선암환자유선암조직급기대응적암방정상유선조직중STS단백화mRNA적표체수평,병진행비교,동시분석STS단백표체여림상병리특정지간적관계.결과: STS단백주요표체우유선암세포화정상유선조직상피세포적세포질내,유3례환자유선암세포적세포핵중가견STS단백적표체,유선간질조직미견STS단백표체.유선암조직급정상유선조직중STS단백적표체수평여기mRNA적표체수평균정정상관.유선암조직중STS단백적양성표체솔(70.0%)명현고우기대응적정상유선조직(42.5%),차이유통계학의의(P=0.013).분층분석현시,절경전、림파결전이화병리분기교만적유선암환자,기유선암조직중STS단백적양성표체솔명현고우기대응적정상유선조직(P<0.05).결론:유선암조직가능통과고표체STS이촉진자신합성자격소,제고종류국부적자격소수평,종이촉진종류적생장화전이.동시,수착종류적생장,종류국부합성적자격소재종류생장중적작용가능월래월중요.
Objective:To investigate the mRNA and protein expressions of steroid sulfatase (STS) in breast cancer tissues and normal breast tissues, and analyze its relationship with clinicopathologic characteristics. Methods:The mRNA and protein expressions of STS, in 40 cases of breast cancer tissues and corresponding paracancerous normal breast tissues, were examined by reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemistry. The correlation of STS expression level with clinicopathologic characteristics was analyzed. Results:STS protein was mainly expressed in the cytoplasm of breast carcinoma cells and epithelial cells in normal breast glands, but not in the stroma. It could be detected in the nucleus of carcinoma cells in 3 cases of breast cancer tissues, which was pathologically classified as invasive ductal carcinoma, invasive lobular carcinoma, and invasive micropapillary carcinoma. STS was not observed in interstitial tissues of breast glands. STS protein expression had positive correlation with its mRNA expressing level. The positivity of STS was 70.0% in breast cancer tissues, significantly higher than that of normal breast tissues (42.5%). The difference was significant (P =0.013). Stratified analysis showed that the positive rates of STS protein were significantly higher in premenopausal patients, the patients with lymph node metastasis, and those with advanced breast carcinoma than those in the matched normal breast tissues (P<0.05). Conclusion:Breast cancer tissues highly expressed STS protein to stimulate local estrogen production, thereby enhancing the progression and migration of breast cancer cells. In addition, as the tumor growth, locally biosynthesized estrogens may play more and more important roles.
