CAJ | 학술논문

由Ralstonia solanacearum E.F.Smith引起的青枯病是若干亚洲和非洲国家花生生产的重要限制因子,利用抗病品种是防治这一病害最好的措施.虽然一大批抗青枯病花生种质资源材料已被鉴定出来,但对其遗传多样性没有足够的研究,限制了在育种中的有效利用.本研究以31份对青枯病具有不同抗性的栽培种花生种质为材料,通过简单序列重复(SSR)和扩增片段长度多态性(AFLP)技术分析了它们的遗传多样性.通过78对SSR引物和126对AFLP引物的鉴定,筛选出能显示抗青枯病种质多态性的SSR引物29对和AFLP引物32对.所选用的29对多态性SSR引物共扩增91条多态性带,平均每对引物扩增3.14条多态性带;32对多态性AFLP引物共扩增72条多态性带,平均扩增2.25条多态性带.在所筛选引物中,4对SSR引物(14H06,7G02,3A8,16C6)和1对AFLP引物(P1M62)检测花生多态性的效果优于其他引物.SSR分析获得的31个花生种质的遗传距离为0.12-0.94,平均为0.53,而AFLP分析获得的遗传距离为0.06～0.57,平均为0.25,基于SSR分析的遗传距离大于基于AFLP分析的遗传距离,疏枝亚种组的遗传分化相对大于密枝亚种组.基于两种分析方法所获得的聚类结果基本一致,但SSR数据聚类结果与栽培种花生的形态分类系统更为吻合.根据分析结果,对构建青枯病抗性遗传图谱群体的核心亲本和抗性育种策略提出了建议.
유Ralstonia solanacearum E.F.Smith인기적청고병시약간아주화비주국가화생생산적중요한제인자,이용항병품충시방치저일병해최호적조시.수연일대비항청고병화생충질자원재료이피감정출래,단대기유전다양성몰유족구적연구,한제료재육충중적유효이용.본연구이31빈대청고병구유불동항성적재배충화생충질위재료,통과간단서렬중복(SSR)화확증편단장도다태성(AFLP)기술분석료타문적유전다양성.통과78대SSR인물화126대AFLP인물적감정,사선출능현시항청고병충질다태성적SSR인물29대화AFLP인물32대.소선용적29대다태성SSR인물공확증91조다태성대,평균매대인물확증3.14조다태성대;32대다태성AFLP인물공확증72조다태성대,평균확증2.25조다태성대.재소사선인물중,4대SSR인물(14H06,7G02,3A8,16C6)화1대AFLP인물(P1M62)검측화생다태성적효과우우기타인물.SSR분석획득적31개화생충질적유전거리위0.12-0.94,평균위0.53,이AFLP분석획득적유전거리위0.06～0.57,평균위0.25,기우SSR분석적유전거리대우기우AFLP분석적유전거리,소지아충조적유전분화상대대우밀지아충조.기우량충분석방법소획득적취류결과기본일치,단SSR수거취류결과여재배충화생적형태분류계통경위문합.근거분석결과,대구건청고병항성유전도보군체적핵심친본화항성육충책략제출료건의.
Bacterial wilt (BW) caused by Ralstonia solanacearum is an important constraint to peanut (Arachis hypogaea L.) production in several Asian and African countries, and planting BW-resistant cultivars is the most feasible method for controlling the disease. Although several BW-resistant peanut germplasm accessions have been identified, the genetic diversity among these has not been properly investigated, which has impeded efficient utilization. In this study, the genetic relationships of 31 peanut genotypes with various levels of resistance to BW were assessed based on SSR and AFLP analyses. Twenty-nine of 78 SSR primers and 32 of 126 AFLP primer combinations employed in this study were polymorphic amongst the peanut genotypes tested. The SSR primers amplified 91 polymorphic loci in total with an average of 3.14 alleles per primer, and the AFLP primers amplified 72 polymorphic loci in total with an average of 2.25 alleles per primer. Four SSR primers (14H06, 7G02, 3A8, 16C6) and one AFLP primer(P1M62) were found to be most efficient in detecting diversity. The genetic distance between pairs of genotypes ranged from 0.12to 0.94 with an average of 0.53 in the SSR data and from 0.06 to 0.57 with an average of 0.25 in the AFLP data. The SSR-based estimates of the genetic distance were generally larger than that based on the AFLP data. The genotypes belonging to subsp, fastigiata possessed wider diversity than that of subsp, hypogaea. The clustering of genotypes based on the SSR and AFLP data were similar but the SSR clustering was more consistent with morphological classification of A. hypogaea. Optimum diverse genotypes of both subsp, hypogaea and subsp, fastigiata can be recommended based on this analysis for developing mapping populations and breeding for high yielding and resistant cultivars.