CAJ | 학술논문

应用电感耦合等离子发射光谱仪、H-600型透射电子显微镜研究了"不知火"杂柑叶片异常黄化及其对解剖特征的影响.主要结果表明:"不知火"黄化叶的Zn、Mg和Ca含量均处于低量水平,且均显著低于"蒰柑";而其SOD、POD和CAT活性、MDA含量均相对高于其正常叶."不知火"黄化叶叶肉细胞的细胞核核膜模糊降解,叶绿体数目明显减少,一些叶绿体形态发生肿胀、呈球形,类囊体片层结构模糊,叶绿体基质中质体小球数量显著增多,细胞质中出现了多泡体.本文还就"不知火"叶片异常黄化的原因及其对解剖特征的影响进行了讨论.
응용전감우합등리자발사광보의、H-600형투사전자현미경연구료"불지화"잡감협편이상황화급기대해부특정적영향.주요결과표명:"불지화"황화협적Zn、Mg화Ca함량균처우저량수평,차균현저저우"반감";이기SOD、POD화CAT활성、MDA함량균상대고우기정상협."불지화"황화협협육세포적세포핵핵막모호강해,협록체수목명현감소,일사협록체형태발생종창、정구형,류낭체편층결구모호,협록체기질중질체소구수량현저증다,세포질중출현료다포체.본문환취"불지화"협편이상황화적원인급기대해부특정적영향진행료토론.
Leaf abnormal chlorosis of "shiranuhi"(Citrus reticulata ×(C. reticulata×C. sinenesis)) and its effects on anatomical features were investigated with inductively coupled plasma optical emission spectrometry and H-600 transmission electron microscope. The main results showed that Zn, Mg and Ca concentrations in chlorotic leaves from "shiranuhi" were at low levels and significantly lower than those of "ponkan"(C. reticulata), while superoxide dismutase, peroxidase and catalase activities, malondialdehyde contents were relatively high in chlorotic leaves compared with its normal leaves. In mesophyll cells of chlorotic leaves from "shiranuhi", the number of chloroplast significantly decreased, nuclear envelope became blurred, some chloroplasts swelled and their shapes turned to round, thylakoid lamellar structures were vague, and large numbers of plastoglobuli were found in stroma of chloroplast, several multivesicular bodies were additional localized in cytoplasm. Causes of leaf abnormal chlorosis of "shiranuhi" and its effects on anatomical features were discussed herein.