南京航空航天大学学报（英文版）
南京航空航天大學學報（英文版）
남경항공항천대학학보（영문판）
TRANSACTIONS OF NANJING UNIVERSITY OF AERONATICS & ASTRONAUTICS
2010年
1期
13-20
,共8页
许恒毅%Zoraida P.Aguilar%苏怀朋%Benjamin J.Jones%John.D.Dixon%熊勇华%魏华%Andrew Y.Wang
許恆毅%Zoraida P.Aguilar%囌懷朋%Benjamin J.Jones%John.D.Dixon%熊勇華%魏華%Andrew Y.Wang
허항의%Zoraida P.Aguilar%소부붕%Benjamin J.Jones%John.D.Dixon%웅용화%위화%Andrew Y.Wang
原位处理%量子点%乳腺癌细胞%非特异性吸附%免疫组化染色
原位處理%量子點%乳腺癌細胞%非特異性吸附%免疫組化染色
원위처리%양자점%유선암세포%비특이성흡부%면역조화염색
in situ processing%quantum optics%breast cancer cells%non-specific binding%immuno-histochemical imaging
乳腺癌是癌症中引起妇女死亡的首要因素.本文采用绿色量子点作为荧光显色材料,建立了一种用于早期乳腺癌诊断的原位成像技术.利用生物素标记的抗HER2/neu抗体与乳腺癌细胞表面表达的HER2结合的特点,然后用偶联绿色量子点的链霉亲和素作为荧光检测标记物,与HER2抗原抗体复合物中的生物素发生特异结合,检测出乳腺癌细胞的存在.结果表明,在荧光显微镜下用绿色量子点原位显色的乳腺癌细胞,相对有机染料组显色亮度增强,故检测灵敏度有所提高.
乳腺癌是癌癥中引起婦女死亡的首要因素.本文採用綠色量子點作為熒光顯色材料,建立瞭一種用于早期乳腺癌診斷的原位成像技術.利用生物素標記的抗HER2/neu抗體與乳腺癌細胞錶麵錶達的HER2結閤的特點,然後用偶聯綠色量子點的鏈黴親和素作為熒光檢測標記物,與HER2抗原抗體複閤物中的生物素髮生特異結閤,檢測齣乳腺癌細胞的存在.結果錶明,在熒光顯微鏡下用綠色量子點原位顯色的乳腺癌細胞,相對有機染料組顯色亮度增彊,故檢測靈敏度有所提高.
유선암시암증중인기부녀사망적수요인소.본문채용록색양자점작위형광현색재료,건립료일충용우조기유선암진단적원위성상기술.이용생물소표기적항HER2/neu항체여유선암세포표면표체적HER2결합적특점,연후용우련록색양자점적련매친화소작위형광검측표기물,여HER2항원항체복합물중적생물소발생특이결합,검측출유선암세포적존재.결과표명,재형광현미경하용록색양자점원위현색적유선암세포,상대유궤염료조현색량도증강,고검측령민도유소제고.
The breast cancer is the most common cause of cancer death in women.To establish an early stage in situ imaging of breast cancer cells,green quantum dots (QDs) are used as a fluorescent signal generator.The QDs based imaging of breast cancer cells involves anti-HER2/neu antibody for labeling the over expressed HER2 on the surface of breast cancer cells.The complete assay involves breast cancer cells,biotin labeled antibody and streptavidin conjugated QDs.The breast cancer cells are grown in culture plates and exposed to the biotin labeled antibodies,and then exposed to streptavidin labeled QDs to utilize the strong and stable biotin-streptavidin interaction.Fluorescent images of the complete assay for breast cancer cells are evaluated on a microscope with a UV light source.Results show that the breast cancer cells in the complete assay are used as fluorescent cells with brighter signals compared with those labeled by the organic dye using similar parameters and the same number of cells.
