CAJ | 학술논문

[目的]对从内蒙古自治区呼伦贝尔盟海拉尔市苜蓿根际土壤中分离出的小杆线虫(Rhabditis(Oscheius)sp.)的生物学特性进行测定.[方法]以大蜡螟(Galleria mellonella)和黄粉甲(Tenebrio molitor)老熟幼虫为寄主,测定小杆线虫的侵染力;以水浴方法测定小杆线虫的耐热能力.[结果]小杆线虫在10、20和40 IJs/虫的剂量下侵染力较低;但在80、160、320和640 IJs/虫的剂量下侵染力较高,侵染大蜡螟96 h、侵染黄粉甲120 h的校正死亡率均达90%以上;在80 IJs/虫的剂量下侵染力最高,侵染120 h,寄主的校正死亡率达100%.在所测试的较高剂量下,小杆线虫对黄粉虫的侵染效果低于对大蜡螟的侵染效果.侵染大蜡螟达到90%以上校正死亡率的侵染效果需96 h,而侵染黄粉虫达到同样的效果则需120 h,浓度1 000～5 000 IJ/ml的小杆线虫在36℃条件下水浴2 d,有25%左右的个体存活,水浴6 d,仍有3%～9%的个体存活,至第9天,线虫个体全部死亡;在38℃下水浴6 h,40℃下水浴2 h,线虫个体全部死亡.[结论]小杆线虫具有较强的侵染力和耐热能力,具有开发应用潜能.
[목적]대종내몽고자치구호륜패이맹해랍이시목숙근제토양중분리출적소간선충(Rhabditis(Oscheius)sp.)적생물학특성진행측정.[방법]이대사명(Galleria mellonella)화황분갑(Tenebrio molitor)노숙유충위기주,측정소간선충적침염력;이수욕방법측정소간선충적내열능력.[결과]소간선충재10、20화40 IJs/충적제량하침염력교저;단재80、160、320화640 IJs/충적제량하침염력교고,침염대사명96 h、침염황분갑120 h적교정사망솔균체90%이상;재80 IJs/충적제량하침염력최고,침염120 h,기주적교정사망솔체100%.재소측시적교고제량하,소간선충대황분충적침염효과저우대대사명적침염효과.침염대사명체도90%이상교정사망솔적침염효과수96 h,이침염황분충체도동양적효과칙수120 h,농도1 000～5 000 IJ/ml적소간선충재36℃조건하수욕2 d,유25%좌우적개체존활,수욕6 d,잉유3%～9%적개체존활,지제9천,선충개체전부사망;재38℃하수욕6 h,40℃하수욕2 h,선충개체전부사망.[결론]소간선충구유교강적침염력화내열능력,구유개발응용잠능.
[Objective]The study was aimed to determine the biological characteristics of Rhabditis(Oscheius)sp.isolated from a rhizosphere soil of alfalfa field in Hailar City,Hulunbuir League,Inner Mongolia Autonomous Region.[Method]The last instar larvae of Galleria mellonella and Tenebrio molitor were used as insect hosts.The infectivity of nematode was determined,and its heat tolerance ability was measured by using water bath method.[Result]The infectivity of the nematodes was lower at the dosages of 10,20 and 40 IJs/larva,but higher at the dosages of 80,160,320 and 640 IJs/larva.The corrected mortality(CM)of G.mellonella at 96 h and T.molitorat 120 h were both above 90%.The nematode infectivity was highest at the dosage of 80 IJs/larva when the hosts' CM reached 100% after 120 h.About 25% nematode individuals could survive in 36 ℃ water bath for 2 d in the densities of 1 000-5 000 IJ/ml,after 6 d,there were still 3%-9% individuals survived,until 9 d the nematodes died totally.A hundred percent of the nematode individuals died in 38 ℃ and 40 ℃ water bath after 6 h and 2 h,respectively.[Conclusion]Rhabditis(O.)sp.has a higher potential to cause the host mortality and represents heat tolerance up to 36 ℃.