CAJ | 학술논문

利用透射电镜观察大竹蛏Solen grandis(Dunker)精子发牛和精子的超微结构,描述了从精原细胞发育到成熟精子过程中超微结构的变化.大竹蛏精子发生历经精原细胞、初级精母细胞、次级精母细胞、精细胞和成熟精子5个阶段.成熟精子属典型的原牛型,全长52-57μm,由头部、中段和尾部3部分组成.头部由顶体和细胞核组成;中段南4到5个椭圆形线粒体和2个相互垂直的中心粒组成;尾部细长,为典型的"9+2"型结构.大竹蛏与同属的长竹蛏精子发生和精子超微结构存在差异.大竹蛏精细胞前顶体囊的高电子密度物质分布在周缘呈一个带缺口的弧形而不是圆形,而长竹蛏前顶体囊周缘的高电子密度物质呈一圆形分布;大竹蛏的前顶体囊是先内凹变形,然后一边变形一边移动,而长竹蛏的前顶体囊是在到达核前端后才开始变形.大竹蛏的顶体比长竹蛏的稍长;大竹蛏精子核形似子弹头而长竹蛏精子核形为圆球状;大竹蛏有卫星体结构而长竹蛏无卫星体结构:大竹蛏精子尾部鞭毛比长竹蛏的长.结果可以为竹蛏科相似种类的鉴定及亲缘关系的探讨提供参考依据.
이용투사전경관찰대죽정Solen grandis(Dunker)정자발우화정자적초미결구,묘술료종정원세포발육도성숙정자과정중초미결구적변화.대죽정정자발생력경정원세포、초급정모세포、차급정모세포、정세포화성숙정자5개계단.성숙정자속전형적원우형,전장52-57μm,유두부、중단화미부3부분조성.두부유정체화세포핵조성;중단남4도5개타원형선립체화2개상호수직적중심립조성;미부세장,위전형적"9+2"형결구.대죽정여동속적장죽정정자발생화정자초미결구존재차이.대죽정정세포전정체낭적고전자밀도물질분포재주연정일개대결구적호형이불시원형,이장죽정전정체낭주연적고전자밀도물질정일원형분포;대죽정적전정체낭시선내요변형,연후일변변형일변이동,이장죽정적전정체낭시재도체핵전단후재개시변형.대죽정적정체비장죽정적초장;대죽정정자핵형사자탄두이장죽정정자핵형위원구상;대죽정유위성체결구이장죽정무위성체결구:대죽정정자미부편모비장죽정적장.결과가이위죽정과상사충류적감정급친연관계적탐토제공삼고의거.
The uhrastructure of spermatogenesis and spermatozoon of Solen granclis was observed by transmission electronic microscope. The results showed ultrastructural changes from spermatogonnium to spermatozoa. The spermatogenesis of Solen grandis included five stages: spermatogonium, spermatocyte Ⅰ, spermatocyte Ⅱ, spermatid and spermatozoon. The spermatozoon, of a typical primitive type (52-57μm), consisted of a head, a middle piece and a tail. The head comprised of a bowl-shaped acrosome and a warhead-shaped nuclear. Four or five ellipsoidal mitochondria and two centrioles made up the middle piece. The tail was thin but long, with a typical structure as type "9+2." The distinctions of spermatogenesis and sperm morphology between Solen grandis and Solen strictus were also discussed. High-density material lying at the circumference of a proacrosomal vesicle was arched with a gap in Solen grandis, but round in Solen strictus. From this gap a proacrosomal vesicle of Solen grandis began to invaginate and deform while it moved to the nuclear front. However a proacrosomal vesicle of Solen strictus did not deform until it reached the nuclear front. Proacrosome of Solen grandis was longer than Solen strictus. The nuclear of Solen grand/s was warhead-shaped while one of Solen strictus was spherical. A satellite body was found in Solen grandis but not in Solen strictus. In addition, Solen grandis had a longer tail than Solen strictus. The results can provide reference for identification and genetic relationship analysis of Solenidae.