CAJ | 학술논문

哺乳动物体细胞核移植技术在农业、生物技术、医药生产和濒危动物保护等方面具有很大的潜力和应用价值,已成为目前发育生物学研究的重要方法.但是核重编程仍是核移植技术的关键因素,制约了重构胚胎干细胞的研究.只有供核发生完全重编程,重构胚胎才能正常发育.核重编程与供核者的年龄,供核细胞的组织来源、分化状态、细胞周期、传代次数,供核细胞的表观遗传标记以及供卵者的年龄、卵子的成熟度等因素有关.创造各种适于核重编程的条件有利于从更高的起点开展核移植胚胎干细胞的研究,提高重构胚胎干细胞建系效率.
포유동물체세포핵이식기술재농업、생물기술、의약생산화빈위동물보호등방면구유흔대적잠력화응용개치,이성위목전발육생물학연구적중요방법.단시핵중편정잉시핵이식기술적관건인소,제약료중구배태간세포적연구.지유공핵발생완전중편정,중구배태재능정상발육.핵중편정여공핵자적년령,공핵세포적조직래원、분화상태、세포주기、전대차수,공핵세포적표관유전표기이급공란자적년령、란자적성숙도등인소유관.창조각충괄우핵중편정적조건유리우종경고적기점개전핵이식배태간세포적연구,제고중구배태간세포건계효솔.
Mammal somatic cell nuclear transfer(SCNT)technology has very big potential and application value in agriculture,biological technology,medicine production and endanger animal.At present,SCNT has become an importarit method in developmental biology research.However,nuclear reprogramming standing in the way of reconstructed embryo stem cell research is still an important factor in the procedure of nuclear transfer and has a predominant effect on reconstructed embryo development. There are many influencing factots influencing on nuclear reprogramming,such as the age of somatic cell donor,the source of somatic cell and its differentiation,cycle stage,passage number and epigenetic marks,the maturation of oocytes and the donor's age ere.Anyway,setting up a smart situation for nuclear reprogramming will improve the efficiency of producing SCNT-ES cell line and benefit the therapeutic cloning.