CAJ | 학술논문

建立了HPLC法测定人血浆中的茶碱和多索茶碱.采用C_(18)色谱柱,以咖啡因为内标,甲醇-水(23:77)为流动相,检测波长273 nm.血浆经甲醇沉淀蛋白后直接进样.茶碱和多索茶碱在2.5～40μg/ml浓度范围内线性关系良好,日内、日间RSD均小于5.2%.
건립료HPLC법측정인혈장중적다감화다색다감.채용C_(18)색보주,이가배인위내표,갑순-수(23:77)위류동상,검측파장273 nm.혈장경갑순침정단백후직접진양.다감화다색다감재2.5～40μg/ml농도범위내선성관계량호,일내、일간RSD균소우5.2%.
An HPLC method was established for the determination of theophyUine and doxofyline in human plasma.A C_(18) column was used,with caffeine as internal standard and the mobile phase of methanol-water(23:77)at 273 nm.The plasma samples were injcoted directly after precipitation by methan01.The calibration curves of theophylline and doxofylline were linear in the concentration range of 2.5-40μg/ml.The intra-and inter-day RSDs WgTe less than 5.2%.