国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2010年
1期
24-26,30
,共4页
快速老化%SAM-P/8小鼠%海马脑片%CA1区%自发放电频率
快速老化%SAM-P/8小鼠%海馬腦片%CA1區%自髮放電頻率
쾌속노화%SAM-P/8소서%해마뇌편%CA1구%자발방전빈솔
Fast aging%SAM-P/8 mouse%Hippocampus slice%CA1%Firing-rate
目的 研究快速老化对记忆脑区海马CA1神经元电活动兴奋性的影响.方法 应用脑片和细胞外记录技术,记录快速老化(SAM-P/8)组和正常对照组小鼠在海马脑片CA1区的锥体神经元自发放电序列,通过计算2组神经元自发放电频率和神经元放电间隔(ISI)研究快速老化对海马CA1区神经元兴奋性的影响.结果 快速老化组小鼠海马CA1区神经元自发放电频率为(1.052±0.364)Hz(样本数n_2=14),正常对照组为4.416±1.306 Hz(样本数n_1=22),前者比后者显著降低(P<0.05);快速老化组ISI≥1 s,占80.5%,正常对照组ISI均≤1 s,其中95.6%≤0.5 s,前者比后者显著延长.结论 快速老化组小鼠海马脑片CA1区神经元的发放频率降低,ISI延长.提示快速老化对小鼠海马区神经元兴奋性电活动起到了明显的抑制作用.
目的 研究快速老化對記憶腦區海馬CA1神經元電活動興奮性的影響.方法 應用腦片和細胞外記錄技術,記錄快速老化(SAM-P/8)組和正常對照組小鼠在海馬腦片CA1區的錐體神經元自髮放電序列,通過計算2組神經元自髮放電頻率和神經元放電間隔(ISI)研究快速老化對海馬CA1區神經元興奮性的影響.結果 快速老化組小鼠海馬CA1區神經元自髮放電頻率為(1.052±0.364)Hz(樣本數n_2=14),正常對照組為4.416±1.306 Hz(樣本數n_1=22),前者比後者顯著降低(P<0.05);快速老化組ISI≥1 s,佔80.5%,正常對照組ISI均≤1 s,其中95.6%≤0.5 s,前者比後者顯著延長.結論 快速老化組小鼠海馬腦片CA1區神經元的髮放頻率降低,ISI延長.提示快速老化對小鼠海馬區神經元興奮性電活動起到瞭明顯的抑製作用.
목적 연구쾌속노화대기억뇌구해마CA1신경원전활동흥강성적영향.방법 응용뇌편화세포외기록기술,기록쾌속노화(SAM-P/8)조화정상대조조소서재해마뇌편CA1구적추체신경원자발방전서렬,통과계산2조신경원자발방전빈솔화신경원방전간격(ISI)연구쾌속노화대해마CA1구신경원흥강성적영향.결과 쾌속노화조소서해마CA1구신경원자발방전빈솔위(1.052±0.364)Hz(양본수n_2=14),정상대조조위4.416±1.306 Hz(양본수n_1=22),전자비후자현저강저(P<0.05);쾌속노화조ISI≥1 s,점80.5%,정상대조조ISI균≤1 s,기중95.6%≤0.5 s,전자비후자현저연장.결론 쾌속노화조소서해마뇌편CA1구신경원적발방빈솔강저,ISI연장.제시쾌속노화대소서해마구신경원흥강성전활동기도료명현적억제작용.
Objective To investigate the effect of fast-aging on the excitability of hippocampus CA1 neurons in mouse and the possible interaction between fast-aging and hippocampus. Methods Using brain slice and extracellular recording technique to record the firing of hippocampus CA1 neurons in fast-aging(SA M-P/8) and normal control mice, after preprocessing, neural firing train were obtained. Using neural firing rate and inter-spike-inter to investigate interaction between fast-aging and hippocampal neural firing. Results The neural firing rate of hippocampus CA1 neurons in fast-aging mice is (1.052±0.364) Hz(n=14), while the neural firing ratein normal control mice is (4.416±1.306) Hz(n=22). In fast-aging mice, 80.5% inter spike intervel(ISI) is longerthan 1sec, but in normal control mice, 95.6% ISI is shorter than 0.5sec. Conclusion The decreased firing rate of hippocampus CA1 and longer ISI observed in the fast-aging mice indicates that fast-aging significantly inhibit hippocampal CA1 neurons excitability.
