中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2008年
2期
125-128
,共4页
郭雪君%李健%倪培华%任涟萍%徐卫国
郭雪君%李健%倪培華%任漣萍%徐衛國
곽설군%리건%예배화%임련평%서위국
哮喘%T淋巴细胞%基因表达调控
哮喘%T淋巴細胞%基因錶達調控
효천%T림파세포%기인표체조공
Asthma%T-Lymphocytes%Gene expression regulation
目的 探讨T淋巴细胞活化衔接子(LAT)及其上游调控因子(Syk、Lck和ZAP-70)在支气管哮喘(简称哮喘)患者外周血T淋巴细胞中的转录表达水平是否存在异常.方法 对20例哮喘患者(哮喘组)及20例非特应症对照者(对照组)采用逆转录-聚合酶链反应(RT-PCR)法检测外周静脉血T淋巴细胞的LAT及Lck、Syk和ZAP-70 mRNA的表达,有关LAT基因转录的结果通过实时定量RT-PCR法进行验证.统计学处理采用SPSS 11.5软件.数据以-x±s表示.组间比较采用t检验.结果 哮喘组患者外周血T淋巴细胞LAT基因的mRNA表达水平为0.54±0.14,对照组为0.72±0.17,两组比较差异有统计学意义(t=3.11,P＜0.01);实时定量RT-PCR法(0.0065±0.0066)证实哮喘患者外周血T淋巴细胞LAT转录水平较对照组(0.0124±0.0045)下调(t=0.0022,P＜0.01).20例哮喘患者Lek和ZAP-70基因mRNA表达水平分别为0.71±0.16、1.05±0.41,对照组分别为0.53±0.17、0.82±0.27.两组比较差异有统计学意义(t值分别为3.18、2.10,P分别＜0.01、＜0.05);哮喘患者Syk基因mRNA表达水平为1.16±0.42,对照组为1.24±0.34,两组间Syk基因转录水平比较差异无统计学意义(t=0.22,P＞0.05).结论 哮喘患者外周血T淋巴细胞LAT基因转录水平下调可能与上游调控因子Lck和ZAP-70基因转录水平上调有关,LAT及上游调控因子Lck和ZAP-70转录水平异常可能是哮喘发病机制之一.
目的 探討T淋巴細胞活化銜接子(LAT)及其上遊調控因子(Syk、Lck和ZAP-70)在支氣管哮喘(簡稱哮喘)患者外週血T淋巴細胞中的轉錄錶達水平是否存在異常.方法 對20例哮喘患者(哮喘組)及20例非特應癥對照者(對照組)採用逆轉錄-聚閤酶鏈反應(RT-PCR)法檢測外週靜脈血T淋巴細胞的LAT及Lck、Syk和ZAP-70 mRNA的錶達,有關LAT基因轉錄的結果通過實時定量RT-PCR法進行驗證.統計學處理採用SPSS 11.5軟件.數據以-x±s錶示.組間比較採用t檢驗.結果 哮喘組患者外週血T淋巴細胞LAT基因的mRNA錶達水平為0.54±0.14,對照組為0.72±0.17,兩組比較差異有統計學意義(t=3.11,P＜0.01);實時定量RT-PCR法(0.0065±0.0066)證實哮喘患者外週血T淋巴細胞LAT轉錄水平較對照組(0.0124±0.0045)下調(t=0.0022,P＜0.01).20例哮喘患者Lek和ZAP-70基因mRNA錶達水平分彆為0.71±0.16、1.05±0.41,對照組分彆為0.53±0.17、0.82±0.27.兩組比較差異有統計學意義(t值分彆為3.18、2.10,P分彆＜0.01、＜0.05);哮喘患者Syk基因mRNA錶達水平為1.16±0.42,對照組為1.24±0.34,兩組間Syk基因轉錄水平比較差異無統計學意義(t=0.22,P＞0.05).結論 哮喘患者外週血T淋巴細胞LAT基因轉錄水平下調可能與上遊調控因子Lck和ZAP-70基因轉錄水平上調有關,LAT及上遊調控因子Lck和ZAP-70轉錄水平異常可能是哮喘髮病機製之一.
목적 탐토T림파세포활화함접자(LAT)급기상유조공인자(Syk、Lck화ZAP-70)재지기관효천(간칭효천)환자외주혈T림파세포중적전록표체수평시부존재이상.방법 대20례효천환자(효천조)급20례비특응증대조자(대조조)채용역전록-취합매련반응(RT-PCR)법검측외주정맥혈T림파세포적LAT급Lck、Syk화ZAP-70 mRNA적표체,유관LAT기인전록적결과통과실시정량RT-PCR법진행험증.통계학처리채용SPSS 11.5연건.수거이-x±s표시.조간비교채용t검험.결과 효천조환자외주혈T림파세포LAT기인적mRNA표체수평위0.54±0.14,대조조위0.72±0.17,량조비교차이유통계학의의(t=3.11,P＜0.01);실시정량RT-PCR법(0.0065±0.0066)증실효천환자외주혈T림파세포LAT전록수평교대조조(0.0124±0.0045)하조(t=0.0022,P＜0.01).20례효천환자Lek화ZAP-70기인mRNA표체수평분별위0.71±0.16、1.05±0.41,대조조분별위0.53±0.17、0.82±0.27.량조비교차이유통계학의의(t치분별위3.18、2.10,P분별＜0.01、＜0.05);효천환자Syk기인mRNA표체수평위1.16±0.42,대조조위1.24±0.34,량조간Syk기인전록수평비교차이무통계학의의(t=0.22,P＞0.05).결론 효천환자외주혈T림파세포LAT기인전록수평하조가능여상유조공인자Lck화ZAP-70기인전록수평상조유관,LAT급상유조공인자Lck화ZAP-70전록수평이상가능시효천발병궤제지일.
Objective To examine the mRNA expression of the linker for activation of T cell (LAT)and its upstream regulatory factors(Syk,Lck and ZAP-7O)in the peripheral blood T cells of asthmatic patients.Methods Reverse transcription polymerase chain reaction(RT-PCR)was used to detect the mRNA expression of LAT and its upstream regulmory factors(Syk,Lck and ZAP-70)in 20 asthmatic patients and 20 nonatlergic subiects.The results of LAT transcription level were confirmed by real-time RT-PCR.Results were expressed as -x±s.Difierences between groups were assessed for significance by t test.Results Compared with nonallergic subjects,a significant decrease of mRNA expression of LAT gene was observed in T cells of asthmatic patients(0.54±0.14 vs 0.72±0.17,t=3.11,P＜0.01),which was verified by real-time RT-PCR(0.0065±0.0066 vs 0.0124±0.0045,t=0.0022,P＜0.01).The Lck and ZAP-70 gene transcription levels were significantly up-regulated(Lck:0.71±0.16 vs 0.53±0.17,t=3.18,P＜0.01;ZAP-70:1.05±0.41 vs 0.82±0.27,t=2.10,P＜0.05).Conclusion A significant decrease of mRNA expression of LAT gene in T cells of asthmatic patients may be due to the up-regulation of its upstream regulatory factors(Lck and ZAP-70).The abnormal mRNA expression of LAT,Lck and ZAP-70 genes may be involved in the pathogenesis of asthma.
