CAJ | 학술논문

采用RT-PCR检测10例甲状腺乳头状腺癌(PTC)和10例癌旁正常甲状腺组织雌激素受体(ER)β1、ERβ2以及ERα的表达,同时用免疫组织化学方法检测107例甲状腺癌、56例甲状腺腺瘤和10例正常甲状腺组织中ERβ1和ERβ2表达,并结合甲状腺癌ERα表达及其它主要临床病理参数进行分析.结果显示,ERα mRNA在PTC组织的表达明显高于癌旁正常组织,ERβ2 mRNA在PTC组织的表达明显低于癌旁正常组织(P＜0.05)；正常甲状腺组织、甲状腺腺瘤和甲状腺癌中3种ER的表达差异均有统计学意义( P＜0.05)；ERβ1和ERβ2在甲状腺癌中的表达与其组织类型有关；甲状腺组织中ERβ1和ERβ2的表达与ERα呈低、中度负相关(ERβ1:r=-0.206,P=0.042；ERβ2:r=-0.545,P＜0.01)；ERβ2的表达还与淋巴结转移以及TNM分期有关.提示ERβ2的表达缺失可能导致甲状腺滤泡上皮细胞的增生和癌变以及肿瘤的演进,并且有一定的预后评估价值.
채용RT-PCR검측10례갑상선유두상선암(PTC)화10례암방정상갑상선조직자격소수체(ER)β1、ERβ2이급ERα적표체,동시용면역조직화학방법검측107례갑상선암、56례갑상선선류화10례정상갑상선조직중ERβ1화ERβ2표체,병결합갑상선암ERα표체급기타주요림상병리삼수진행분석.결과현시,ERα mRNA재PTC조직적표체명현고우암방정상조직,ERβ2 mRNA재PTC조직적표체명현저우암방정상조직(P＜0.05)；정상갑상선조직、갑상선선류화갑상선암중3충ER적표체차이균유통계학의의( P＜0.05)；ERβ1화ERβ2재갑상선암중적표체여기조직류형유관；갑상선조직중ERβ1화ERβ2적표체여ERα정저、중도부상관(ERβ1:r=-0.206,P=0.042；ERβ2:r=-0.545,P＜0.01)；ERβ2적표체환여림파결전이이급TNM분기유관.제시ERβ2적표체결실가능도치갑상선려포상피세포적증생화암변이급종류적연진,병차유일정적예후평고개치.
The expressions of estrogen receptor (ER) α,ERβ1,and ERβ2 in thyroid carcinoma,thyroid adenoma,and adjacent normal thyroid tissue were determined by RT-PCR and immunohistochemistry,and the relationship of expression levels of three ERs and the main clinical pathologic parameters were evaluated.The result showed that ERα mRNA expression in papillary thyroid carcinoma(PTC) tissues was significantly higher than adjacent normal tissue,while,ERβ2 mRNA expression in PTC tissues was significantly lower than normal tissue ( P＜0.05 ).The positive expression of ERβ1 and ERβ2 in the thyroid carcinoma was related with the histological variant of carcinomas; the ERβ2 expressions were related with the lymph node metastasis and TNM staging of the carcinomas.These results suggest that the decreased ERβ2 expression may induce proliferation and carcinogenesis of thyroid follicular epithelial cells as well as progression of the carcinoma,and is a powerful prognostic indicator.