中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2009年
7期
577-581
,共5页
祝凯华%李玉民%刘玲玲%张丹%唐小凡%赵军%何文婷%刘涛%宋玉鑫
祝凱華%李玉民%劉玲玲%張丹%唐小凡%趙軍%何文婷%劉濤%宋玉鑫
축개화%리옥민%류령령%장단%당소범%조군%하문정%류도%송옥흠
胃肿瘤%白细胞介素10%多态性,单核苷酸%螺杆菌,幽门%免疫印迹法
胃腫瘤%白細胞介素10%多態性,單覈苷痠%螺桿菌,幽門%免疫印跡法
위종류%백세포개소10%다태성,단핵감산%라간균,유문%면역인적법
Stomach neoplasms%Iterleukin-10%Polymorphism,single nucleotide%Helicobacter pylori%Immunoblotting
目的 探讨白细胞介素10(IL-10)启动子区1082位点的单核苷酸多态性(singlenucleotide polymorphisms,SNP)、不同类型幽门螺杆菌(Hp)感染与甘肃地区胃癌易感性之间的关系.方法 采用聚合酶链反应-限制性片段长度多态(PCR-RFLP)和PCR产物直接测序技术分别检测来自甘肃地区人群137例胃癌患者及与其配比的144例对照个体及131例胃癌癌前病变IL-10-1082A/G基因多态性;采用免疫印迹技术对Hp进行毒株分型.结果 ①对照人群IL-10-1082位点的AA、AG、GG 3种基因型分布频率分别为76.4%、22.2%和1.4%;Hp感染情况为Hp Ⅰ型16.0%、HpⅡ型31.9%、阴性52.1%.②癌前病变组IL-10-1082位点的AA、AG、GG 3种基因型分布频率分别为63.4%、32.8%和3.8%.AG+GG基因型携带频率高于正常对照组(P=0.018),携带IL-10-1082 AG+GG基因型个体癌前病变的发病风险增高到1.87倍.③胃癌组IL-10-1082位点的从、AG、GG 3种基因型分布频率分别为58.4%、35.8%和5.8%;胃癌组IL-10-1082 AG+GG基因型携带频率显著高于正常对照组(P=0.010),携带IL-10-1082 AG+GG基因型个体胃癌的发病风险增高到2.31倍.④以IL-10-1082从基因型并Hp免疫印迹阴性组为对照,AG+GG基因型并Hp免疫印迹阴性个体、AG+GG基因型并Hp感染个体、AG+GG基因型并Hp Ⅰ型感染个体胃癌患病风险增高,其中AG+GG基因型并Hp Ⅰ型感染个体的患病风险显著增高,为对照组的9.73倍.结论 IL-10-1082位点A/G多态性与胃癌的遗传易感性相关.
目的 探討白細胞介素10(IL-10)啟動子區1082位點的單覈苷痠多態性(singlenucleotide polymorphisms,SNP)、不同類型幽門螺桿菌(Hp)感染與甘肅地區胃癌易感性之間的關繫.方法 採用聚閤酶鏈反應-限製性片段長度多態(PCR-RFLP)和PCR產物直接測序技術分彆檢測來自甘肅地區人群137例胃癌患者及與其配比的144例對照箇體及131例胃癌癌前病變IL-10-1082A/G基因多態性;採用免疫印跡技術對Hp進行毒株分型.結果 ①對照人群IL-10-1082位點的AA、AG、GG 3種基因型分佈頻率分彆為76.4%、22.2%和1.4%;Hp感染情況為Hp Ⅰ型16.0%、HpⅡ型31.9%、陰性52.1%.②癌前病變組IL-10-1082位點的AA、AG、GG 3種基因型分佈頻率分彆為63.4%、32.8%和3.8%.AG+GG基因型攜帶頻率高于正常對照組(P=0.018),攜帶IL-10-1082 AG+GG基因型箇體癌前病變的髮病風險增高到1.87倍.③胃癌組IL-10-1082位點的從、AG、GG 3種基因型分佈頻率分彆為58.4%、35.8%和5.8%;胃癌組IL-10-1082 AG+GG基因型攜帶頻率顯著高于正常對照組(P=0.010),攜帶IL-10-1082 AG+GG基因型箇體胃癌的髮病風險增高到2.31倍.④以IL-10-1082從基因型併Hp免疫印跡陰性組為對照,AG+GG基因型併Hp免疫印跡陰性箇體、AG+GG基因型併Hp感染箇體、AG+GG基因型併Hp Ⅰ型感染箇體胃癌患病風險增高,其中AG+GG基因型併Hp Ⅰ型感染箇體的患病風險顯著增高,為對照組的9.73倍.結論 IL-10-1082位點A/G多態性與胃癌的遺傳易感性相關.
목적 탐토백세포개소10(IL-10)계동자구1082위점적단핵감산다태성(singlenucleotide polymorphisms,SNP)、불동류형유문라간균(Hp)감염여감숙지구위암역감성지간적관계.방법 채용취합매련반응-한제성편단장도다태(PCR-RFLP)화PCR산물직접측서기술분별검측래자감숙지구인군137례위암환자급여기배비적144례대조개체급131례위암암전병변IL-10-1082A/G기인다태성;채용면역인적기술대Hp진행독주분형.결과 ①대조인군IL-10-1082위점적AA、AG、GG 3충기인형분포빈솔분별위76.4%、22.2%화1.4%;Hp감염정황위Hp Ⅰ형16.0%、HpⅡ형31.9%、음성52.1%.②암전병변조IL-10-1082위점적AA、AG、GG 3충기인형분포빈솔분별위63.4%、32.8%화3.8%.AG+GG기인형휴대빈솔고우정상대조조(P=0.018),휴대IL-10-1082 AG+GG기인형개체암전병변적발병풍험증고도1.87배.③위암조IL-10-1082위점적종、AG、GG 3충기인형분포빈솔분별위58.4%、35.8%화5.8%;위암조IL-10-1082 AG+GG기인형휴대빈솔현저고우정상대조조(P=0.010),휴대IL-10-1082 AG+GG기인형개체위암적발병풍험증고도2.31배.④이IL-10-1082종기인형병Hp면역인적음성조위대조,AG+GG기인형병Hp면역인적음성개체、AG+GG기인형병Hp감염개체、AG+GG기인형병Hp Ⅰ형감염개체위암환병풍험증고,기중AG+GG기인형병Hp Ⅰ형감염개체적환병풍험현저증고,위대조조적9.73배.결론 IL-10-1082위점A/G다태성여위암적유전역감성상관.
Objective To analyze the relationship between 1082 site A/G polymorphism in interleukin-10 gene and different virulence factors of Helieobacter pylori in Gansu Province and susceptibility to gastric cancer genesis. Methods Polymerase chain restriction fragment length polymorphism and direct sequencing were performed to analyze the genotype of the A/G polymorphism in its-1082 site of promoter region, and immunoblotting was performed to test different virulence factors antibody of H. pylori. Results respectively in the gastric precancerous lesion group. The frequency of AG + GG genotype was statistically higher in the gastric precancerous lesion group compared to the control group (P =0. 018), the risk gastric frequency of AA, AG, GG genotypes were 58.4% ,35.8% ,5.8%, respectively in the gastric cancer group. The frequency of AG + GG genotype was higher in the gastric cancer group compared to the control group (P =0. 010), moreover, individuals with the IL-10-1082AG + GG genotype rose to 2. 31 fold risk for gastric there was an increased risk of gastric precancerous lesion and developing gastric cancer for those carrying both AG + GG genotype and seropositive H. pylori I, with 9.73 fold risk. Conclusion There was a relationship between IL-10-1082 A/G polymorphism and susceptibility to gastric cancer.
