CAJ | 학술논문

以平均聚合度10以下的寡糖为目标产物,将褐藻胶经不同浓度过氧化氢,在不同温度和反应时间下降解,筛选出最佳降解条件,以此降解条件下制备的寡糖对7种常见细菌和真菌进行抑菌实验.结果表明:过氧化氢体积百分比浓度3%、温度80℃、反应1.5～2 h为最佳降解条件;寡糖对细菌的生长有明显的抑制作用,最低抑菌浓度金黄色葡萄球菌、大肠杆菌及藤黄微球菌为0.625%、枯草芽孢杆菌为2.5%,寡糖对真菌抑制作用不显著.
이평균취합도10이하적과당위목표산물,장갈조효경불동농도과양화경,재불동온도화반응시간하강해,사선출최가강해조건,이차강해조건하제비적과당대7충상견세균화진균진행억균실험.결과표명:과양화경체적백분비농도3%、온도80℃、반응1.5～2 h위최가강해조건;과당대세균적생장유명현적억제작용,최저억균농도금황색포도구균、대장간균급등황미구균위0.625%、고초아포간균위2.5%,과당대진균억제작용불현저.
In order to obtain oligosaccharide with average degree of polymerization 10 below, alginate was degraded by H_2O_2 with different concentrations, at different temperature and reaction time, the best condition of degradation was selected. The oligosaccharides prepared under the best condition were used to carry on antibacterial experiments on seven kinds of common bacteria and fungi. The results showed that H_2O_2 with 3% concentration, temperature at 80℃, reaction time at 1.5-2.0 h was the best degradation condition. Oligosaccharide inhibited the growth of bacteria obviously, the MIC of Staphylococcus aureus, Escherichia coli and Micracaccus luteus were 0.625%, Bacillus subtilis at 2.5%. However, inhibition of fungi was not significant.