检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2010年
2期
126-131
,共6页
混合型高脂血症%肝脂酶%基因多态性
混閤型高脂血癥%肝脂酶%基因多態性
혼합형고지혈증%간지매%기인다태성
Combined hyperlipidemia%Hepatic lipase%Gene polymorphism
目的 探讨混合型高脂血症(CHL)与肝脂酶(HL)基因启动子763A/G多态性的关系.方法 运用聚合酶链反应-限制性内切酶片段长度多态性技术(RCR-RFLP),对219名对照者和218例CHL患者的HL基因启动子763A/G多态性进行检测,并研究其对血脂水平的影响.结果 总研究组的HL-763A/G多态性的AA、AG和GG基因型频率分别为0.315 5、0.493 6、0.190 8;A、G等位基因频率分别为0.562 3、0.437 7.在CHL组中除年龄、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白A-Ⅰ(apo A-Ⅰ),AA型的低密度脂蛋白胆固醇(LDL-C)、载脂蛋白B(apo B)及GG型的apo A-Ⅰ水平外,其余指标均与对照组同基因型之间差异有统计学意义(P<0.05);GG型的年龄明显低于AA型(P<0.05),apo A-Ⅰ水平明显高于AA型(P<0.05),而apo A-Ⅰ及apo A-Ⅰ/apo B水平明显高于AG型(P<0.05).在CHL男性组中,除AA型HDL-C、GG型HDL-C、apo A-Ⅰ水平外,其余血脂水平均高于对照男性组(P<0.05),同样,在CHL女性组中,除GG型的HDL-C、apo A-Ⅰ、非高密度脂蛋白胆固醇(nonHDL-C)、apo B水平外,其余血脂水平均高于对照女性组(P<0.05).而CHL女性组GG型的apo A-Ⅰ水平明显高于AA型(P<0.05).结论 HL启动子763A/G多态性与CHL的发生相关,并可影响血浆的脂类代谢.
目的 探討混閤型高脂血癥(CHL)與肝脂酶(HL)基因啟動子763A/G多態性的關繫.方法 運用聚閤酶鏈反應-限製性內切酶片段長度多態性技術(RCR-RFLP),對219名對照者和218例CHL患者的HL基因啟動子763A/G多態性進行檢測,併研究其對血脂水平的影響.結果 總研究組的HL-763A/G多態性的AA、AG和GG基因型頻率分彆為0.315 5、0.493 6、0.190 8;A、G等位基因頻率分彆為0.562 3、0.437 7.在CHL組中除年齡、高密度脂蛋白膽固醇(HDL-C)、載脂蛋白A-Ⅰ(apo A-Ⅰ),AA型的低密度脂蛋白膽固醇(LDL-C)、載脂蛋白B(apo B)及GG型的apo A-Ⅰ水平外,其餘指標均與對照組同基因型之間差異有統計學意義(P<0.05);GG型的年齡明顯低于AA型(P<0.05),apo A-Ⅰ水平明顯高于AA型(P<0.05),而apo A-Ⅰ及apo A-Ⅰ/apo B水平明顯高于AG型(P<0.05).在CHL男性組中,除AA型HDL-C、GG型HDL-C、apo A-Ⅰ水平外,其餘血脂水平均高于對照男性組(P<0.05),同樣,在CHL女性組中,除GG型的HDL-C、apo A-Ⅰ、非高密度脂蛋白膽固醇(nonHDL-C)、apo B水平外,其餘血脂水平均高于對照女性組(P<0.05).而CHL女性組GG型的apo A-Ⅰ水平明顯高于AA型(P<0.05).結論 HL啟動子763A/G多態性與CHL的髮生相關,併可影響血漿的脂類代謝.
목적 탐토혼합형고지혈증(CHL)여간지매(HL)기인계동자763A/G다태성적관계.방법 운용취합매련반응-한제성내절매편단장도다태성기술(RCR-RFLP),대219명대조자화218례CHL환자적HL기인계동자763A/G다태성진행검측,병연구기대혈지수평적영향.결과 총연구조적HL-763A/G다태성적AA、AG화GG기인형빈솔분별위0.315 5、0.493 6、0.190 8;A、G등위기인빈솔분별위0.562 3、0.437 7.재CHL조중제년령、고밀도지단백담고순(HDL-C)、재지단백A-Ⅰ(apo A-Ⅰ),AA형적저밀도지단백담고순(LDL-C)、재지단백B(apo B)급GG형적apo A-Ⅰ수평외,기여지표균여대조조동기인형지간차이유통계학의의(P<0.05);GG형적년령명현저우AA형(P<0.05),apo A-Ⅰ수평명현고우AA형(P<0.05),이apo A-Ⅰ급apo A-Ⅰ/apo B수평명현고우AG형(P<0.05).재CHL남성조중,제AA형HDL-C、GG형HDL-C、apo A-Ⅰ수평외,기여혈지수평균고우대조남성조(P<0.05),동양,재CHL녀성조중,제GG형적HDL-C、apo A-Ⅰ、비고밀도지단백담고순(nonHDL-C)、apo B수평외,기여혈지수평균고우대조녀성조(P<0.05).이CHL녀성조GG형적apo A-Ⅰ수평명현고우AA형(P<0.05).결론 HL계동자763A/G다태성여CHL적발생상관,병가영향혈장적지류대사.
Objective To explore the correlation between combined hyperlipidemia (CHL) and hepatic lipase (HL) gene promoter 763A/G polymorphism. Methods HL gene promoter 763A/G polymorphism was detected by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) technique in 219 controls and 218 cases of patients with CHL, and its influence on blood lipid levels was analyzed. Results In the total study group of HL-763A/G polymorphism, the frequencies of the genotype AA, AG and GG were 0.315 5,0.493 6,0.190 8, and the frequencies of allele A and G were 0.562 3 and 0.437 7. In the CHL group, except age, high-density lipoprotein cholesterol (HDL-C), apolipoprotein A-Ⅰ (apo A-Ⅰ), AA type low-density lipoprotein cholesterol (LDL-C), apolipoprotein B (apo B) and GG type of apo A-Ⅰ levels, other indicators had statistical significance with the control group between genotypes (P<0.05). The age in GG type was significantly lower than that in AA type(P<0.05), and apo A-Ⅰ levels were significantly higher than those of AA type(P<0.05), while the levels of apo A-Ⅰ and apo A-Ⅰ/apo B were significantly higher than those of AG type (P<0.05). In the CHL male group, except HDL-C of AA type, HDL-C of GG type and apo A-Ⅰ levels, the levels of other lipids were higher than those in the control male group (P<0.05). In the CHL female group, except HDL-C, apo A-Ⅰ, non-high-density lipoprotein cholesterol (nonHDL-C), apo B levels of GG type, the other levels of lipids were higher than those in the control female group (P<0.05). In the CHL female group, the levels of apo A-Ⅰ of GG type were significantly higher than those of the AA type (P<0.05). Conclusions HL promoter 763A/G polymorphism has the relationship with the occurrence of CHL, and may influence plasma lipid metabolism.
