CAJ | 학술논문

为了探讨云南水稻传统品种内不同样本群体间的遗传多样性及其空间分布,以及空间隔离和种子介导的基因流在水稻传统品种遗传多样性的维持过程中的作用,利用24对微卫星(SSR)引物,对来自云南不同地区和村寨的6个水稻传统品种66个不同样本群体的共660份样本进行了遗传多样性和遗传关系的分析.结果表明,24个SSR位点共检测到133个等位基因,都为具有中度或者高度多态的位点,期望杂合度(H_e)为0.662,平均多态信息含量(PIC)为0.615,表明云南水稻传统品种具有较高水平的遗传多样性.分子方差(AMOVA)分析的结果表明云南水稻传统品种的遗传变异主要发生在地区内样本群体间(58%),地区间为(31%),样本群体内具有为11%的遗传多样性.品种间和地区间有一定水平由种子介导的基因流(N_m).聚类分析表明,66个水稻样本群体在遗传距离为1.1水平上分为两大类,基本上相同的品种聚在一起,地理来源相近也聚在一起,但有些品种有明显的地区间交叉.Mantel检验的结果表明,每个品种样本群体的之间遗传距离和空间距离不具相关关系,揭示出云南水稻传统品种群体内的遗传多样性现状可能是其环境异质性、空间隔离、种子介导的基因流综合作用的结果.
위료탐토운남수도전통품충내불동양본군체간적유전다양성급기공간분포,이급공간격리화충자개도적기인류재수도전통품충유전다양성적유지과정중적작용,이용24대미위성(SSR)인물,대래자운남불동지구화촌채적6개수도전통품충66개불동양본군체적공660빈양본진행료유전다양성화유전관계적분석.결과표명,24개SSR위점공검측도133개등위기인,도위구유중도혹자고도다태적위점,기망잡합도(H_e)위0.662,평균다태신식함량(PIC)위0.615,표명운남수도전통품충구유교고수평적유전다양성.분자방차(AMOVA)분석적결과표명운남수도전통품충적유전변이주요발생재지구내양본군체간(58%),지구간위(31%),양본군체내구유위11%적유전다양성.품충간화지구간유일정수평유충자개도적기인류(N_m).취류분석표명,66개수도양본군체재유전거리위1.1수평상분위량대류,기본상상동적품충취재일기,지리래원상근야취재일기,단유사품충유명현적지구간교차.Mantel검험적결과표명,매개품충양본군체적지간유전거리화공간거리불구상관관계,게시출운남수도전통품충군체내적유전다양성현상가능시기배경이질성、공간격리、충자개도적기인류종합작용적결과.
To investigate genetic diversity and its spatial distribution,as well as the mechanism for maintenance of genetic diversity via spatial isolation and seed-mediated gene flow in traditional rice varieties,we analyzed a total of 660 rice plants representing 66 sample populations of 6 traditional varieties from different regions and villages in Yunnan Province for their genetic diversity and genetic relationships,using 24 microsatellite loci.Consequently,a total of 133 alleles were detected from the 24 SSR primer pairs,with medium or high polymorphisms for the SSR loci.The gene diversity as measured by the average expected heterozygosity(H_e)was 0.662 and the polymorphism information content(PIC)of all samples was 0.615,indicating high level of genetic diversity of in rice traditional varieties in Yunnan.The AMOVA indicated that about 58%of genetic variation was detected among varieties within regions,31%of variation among regions,and 11%of variation within sample populations.This suggested a certain level of seed-mediated gene flow(N_m)among sample populations of different varieties and among regions.Cluster analysis showed that the 66 rice sample populations could be divided into two major groups at the genetic distance coefficient of 1.1.In principle,the sample populations belonging to the same variety and having close spatial distance were grouped together,although there are some overlaps of the sample populations.Mantel test demonstrated no evident correlation between genetic distances and spatial distances of the sample populations of each variety.This study suggested that the current status of genetic diversity among rice populations in Yunnan Province could have resulted from the combined effects including environmental seed exchange between and within regions.