CAJ | 학술논문

目的:同时测定不同采收期江香薷药材中木犀草素、黄芩素-7-甲醚的含量.方法:采用Angilent Zorbax,Extend C-18(4.6 mm×250 mm,5 μm)色谱柱;以甲醇-0.4%磷酸水溶液(体积比为50:50)为流动相进行等度洗脱,柱温:35℃;流速:1.0 mL/min;检测波长:350 nm.结果:木犀草素、黄芩素-7-甲醚分别在0.04～0.61 μg、0.20～3.01 μg范围内线性关系良好,相关系数均为0.999 9.药材提取方法的平均回收率分别为99.54%、98.60%,精密度、稳定性及重复性均良好.不同采收期江香薷药材中木犀草素和黄芩素-7-甲醚含量有较大差异,以8月份药材中木犀草素和黄芩素-7-甲醚含量最高,分别为264.8μg/g、2 746.3μg/g.结论:所建立的方法灵敏快速,操作简便,重现性好,可用于江香薷药材的质量控制.
목적:동시측정불동채수기강향유약재중목서초소、황금소-7-갑미적함량.방법:채용Angilent Zorbax,Extend C-18(4.6 mm×250 mm,5 μm)색보주;이갑순-0.4%린산수용액(체적비위50:50)위류동상진행등도세탈,주온:35℃;류속:1.0 mL/min;검측파장:350 nm.결과:목서초소、황금소-7-갑미분별재0.04～0.61 μg、0.20～3.01 μg범위내선성관계량호,상관계수균위0.999 9.약재제취방법적평균회수솔분별위99.54%、98.60%,정밀도、은정성급중복성균량호.불동채수기강향유약재중목서초소화황금소-7-갑미함량유교대차이,이8월빈약재중목서초소화황금소-7-갑미함량최고,분별위264.8μg/g、2 746.3μg/g.결론:소건립적방법령민쾌속,조작간편,중현성호,가용우강향유약재적질량공제.
AIM:To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS: The Angilent Zorbax,Extend C-18 column(4.6 mm×250 mm,5μm) was used with methanol-water (containing 0.4% phosphonic acid)(50: 50) as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS : The method had the good linear relationship within the range of 0.04-0.61 μg for luteolin and within the range of 0.20-3.01 μg for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence (264.8 μg/g,2 746.3 μg/g) in August,respectively.CONCLUSION: The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.