CAJ | 학술논문

背景:课题组的前期工作已证实骨髓间充质干细胞可以诱导分化为血管内皮细胞和血管平滑肌细胞,并证实所诱导的细胞和胶原包埋的聚乙醇酸支架具有良好的组织相容性.目的:探讨利用动物皮下作为生物反应器构建小口径组织工程化血管的可行性.方法:骨髓间充质干细胞诱导分化为血管平滑肌样细胞和血管内皮样细胞,分层种植于胶原包埋聚乙醇酸支架表面,然后将细胞-支架复合体种植于动物皮下,构建小口径组织工程化血管.结果与结论:人工血管组织学观察见管壁结构清晰,其大体结构和天然血管相似,可承受26.6 kPa的血管腔内压力不破裂.皮下培养8周免疫荧光观察Brdu标记的部分细胞核呈现明亮的黄绿色荧光.结果说明利用动物的皮下作为生物反应器,采用静态培养的方式构建小口径组织工程化血管是可行的.
배경:과제조적전기공작이증실골수간충질간세포가이유도분화위혈관내피세포화혈관평활기세포,병증실소유도적세포화효원포매적취을순산지가구유량호적조직상용성.목적:탐토이용동물피하작위생물반응기구건소구경조직공정화혈관적가행성.방법:골수간충질간세포유도분화위혈관평활기양세포화혈관내피양세포,분층충식우효원포매취을순산지가표면,연후장세포-지가복합체충식우동물피하,구건소구경조직공정화혈관.결과여결론:인공혈관조직학관찰견관벽결구청석,기대체결구화천연혈관상사,가승수26.6 kPa적혈관강내압력불파렬.피하배양8주면역형광관찰Brdu표기적부분세포핵정현명량적황록색형광.결과설명이용동물적피하작위생물반응기,채용정태배양적방식구건소구경조직공정화혈관시가행적.
BACKGROUND: Our former studies have shown that bone marrow mesenchymal stem cells (BMMSCs) can be induced differentiation to vascular smooth muscle-like cells (VSMLCs) and vascular endothelium-like cells (VELCs), which are compatible with collagen-embedded polyglycolic acid scaffolds. OBJECTIVE: To investigate the possibility of constructing small diameter tissue-engineered blood vessels via subcutaneous implantation. METHODS: The cells-scaffold complex was produced by separately seeding VSMLCs and VELCs derived from BMMSCs on polyglycolic acid collagen scaffolds. The two layers were separated by ECMgel. The cells-scaffold complex was subcutaneous implanted into small diameter tissue-engineered blood vessels.RESULTS AND CONCLUSION: Histological analysis of the small diameter tissue-engineered blood vessel walls revealed a typical artery structure, which was similar to natural vessels. The tissue-engineered blood vessels were not broken down under a force of 26.6 kPa. Eight weeks after implantation, the Brdu-labeled seed cells were found in the three layers of the vessel walls. The results revealed that the subcutaneous tissue was a good bioreactor to construct small diameter tissue-engineered blood vessels.