CAJ | 학술논문

目的探讨大黄素对3T3-L1小鼠前脂肪细胞增殖分化和对脂肪代谢的影响，以及探讨大黄素可能的减肥作用的药理机制。方法　MTT法测定细胞的增殖速度，油红O染色法测定细胞的分化速度，分光光度法测定脂肪酸合成酶活性。结果　大黄素在较低浓度下对3T3-L1小鼠前脂肪细胞的增殖有一定的促进作用，但当浓度升高时，转化为剂量依赖性抑制作用；大黄素对3T3-L1小鼠前脂肪细胞向脂肪细胞的分化以及对脂肪酸合成酶活性有剂量依赖性抑制作用。结论　本研究在国际上第一次报道大黄素对3T3-L1小鼠前脂肪细胞增殖与分化及脂肪代谢有影响；大黄素可能具有潜在的减肥、降脂作用。
목적탐토대황소대3T3-L1소서전지방세포증식분화화대지방대사적영향，이급탐토대황소가능적감비작용적약리궤제。방법　MTT법측정세포적증식속도，유홍O염색법측정세포적분화속도，분광광도법측정지방산합성매활성。결과　대황소재교저농도하대3T3-L1소서전지방세포적증식유일정적촉진작용，단당농도승고시，전화위제량의뢰성억제작용；대황소대3T3-L1소서전지방세포향지방세포적분화이급대지방산합성매활성유제량의뢰성억제작용。 결론　본연구재국제상제일차보도대황소대3T3-L1소서전지방세포증식여분화급지방대사유영향；대황소가능구유잠재적감비、강지작용。
Objective To study the effects of emodin on proliferation and differentiation of 3T3-L1 preadipocyte and the possible mechanism.Methods Cell proliferation was determined by MTT spectrophotometry, cell differentiation was determined by Oil Red O staining, and fatty acid synthase (FAS) activity was determined by spectrophotometry. Results Emodin promoted proliferation of 3T3-L1 preadipocyte at low concentration and inhibited the proliferation at high concentration in a dose-related manner. In contrast, it inhibited cell differentiation into adipocyte at low concentration in a dose-related manner. In vitro emodin inhibited the activity of FAS in a dose-related manner.Conclusions The effects of emodin on 3T3-L1 cell's proliferation and differentiation are dose dependent. Emodin inhibits the activity of FAS. Our results suggest that emodin should have a potential to serve as a fat-reducing drug.