CAJ | 학술논문

目的探讨电刺激迷走神经对内毒素(lipopolysaccharide,LPS)休克兔生命体征变化的影响.方法 16只兔随机分为刺激组(S组)和对照组(C组).两组均切断舣侧颈迷走神经干,按600μg/kg静注大肠杆菌LPS.S组选择左迷走神经近心端接刺激电极,在注射LPS前后各持续电刺激(电压10 V,频率5 Hz,波宽5 ms)10 min.分别于输注LPS前及后30,60,120,180,240和300 min记录心率(HR)和平均动脉压(MABP),检测血清中肿瘤坏死凶子(TNF-α)和白介素10(IL-10)含量.结果兴奋迷走神经能缓解LPS引起的低血压休克,同时与C组比较,S组血清中TNF-α显著降低[(38.12±7.85)ps/mL vs.(55.12±7.89)ps/mL,P<0.01]而IL-10水平明显升高[(55.12±9.37)pg/mL vs.(40.15±5.44)ps/mL,P<0.01].结论兴奋迷走神经可以改善LPS休克兔的生命体征,这种现象可能与迷走神经影响体内炎症介质的合成有关.
목적 탐토전자격미주신경대내독소(lipopolysaccharide,LPS)휴극토생명체정변화적영향.방법 16지토수궤분위자격조(S조)화대조조(C조).량조균절단의측경미주신경간,안600μg/kg정주대장간균LPS.S조선택좌미주신경근심단접자격전겁,재주사LPS전후각지속전자격(전압10 V,빈솔5 Hz,파관5 ms)10 min.분별우수주LPS전급후30,60,120,180,240화300 min기록심솔(HR)화평균동맥압(MABP),검측혈청중종류배사흉자(TNF-α)화백개소10(IL-10)함량.결과 흥강미주신경능완해LPS인기적저혈압휴극,동시여C조비교,S조혈청중TNF-α현저강저[(38.12±7.85)ps/mL vs.(55.12±7.89)ps/mL,P<0.01]이IL-10수평명현승고[(55.12±9.37)pg/mL vs.(40.15±5.44)ps/mL,P<0.01].결론흥강미주신경가이개선LPS휴극토적생명체정,저충현상가능여미주신경영향체내염증개질적합성유관.
Objective To study the effects of electrical stimulation of efferent vagus nerve on the endotoxic shock in rabbits. Method Sixteen Newzealand rabbits were randomly divided into 2 groups, namely group S as the stimulation group and group C as the control group). Rabbits were subjected to bilateral cervical vagotomy and had challenge with intravenous injection of lipopolysaccharide (LPS) (E. COLI O111: B4 , DIFCO, USA) in a dose of 600 μg/kg. The distal end of the left vagus nerve trunk was connected to an electric stimulator with bipolar electrode and controlled by an acquisition system. Stimuli with stable voltage (10 V,5 Hz,5 ms) were applied twice to the nerve for 10 minutes just before and after the administration of LPS in group S. At the time before and after the infusion of LPS 30 min,60 min, 120 min, 180 min,240 min and 300 min respectively, the heart rate(HR)and the mean arterial blood pressure (MABP) in each animal were recorded, and blood samples were taken for measuring serum tumor necrosis factor-αa(TNF-α) and interleukin-10 (IL-10). Results Compared with group C,the electrical stimulation of efferent vagus nerve could significantly attenuated the LPS-induced hypotension and de-creased the contents of TNF-α[(38.12±7.85) pg/mL vs. (55.12±7.89) pg/mL, P <0.01], but increased the contents of IL-10[(55.12±9.37)pg/mL vs. (40.15±5.44) pg/mL, P <0.01]afar LPS challenge. Conclusions The stimulation of the efferent vagus nerve can down-regulate systemic TNF-α, production and attenu-ate the development of shock after LPS challenge.