CAJ | 학술논문

目的探讨乙酰肝素酶及E-钙黏蛋白对胃癌侵袭转移的影响.方法收集2005年2月至2007年5月辽宁省肿瘤医院手术切除的50例胃癌标本.采用RT-PCR法检测乙酰肝素酶mRNA及E-钙黏蛋白mRNA在胃癌组织中的表达,同时应用免疫组织化学法检测E-钙黏蛋白在胃癌组织中的表达.计量资料采用方差分析和t检验;计数资料采用χ~2检验.结果乙酰肝素酶与E-钙黏蛋白的表达在胃癌的细胞分化程度、淋巴结转移、TNM分期方面比较,差异有统计学意义(t=1.999,4.258,1.735;1.286,6.794,3.091;χ~2=6.273,9.397,5.640,P<0.05).乙酰肝素酶阳性/E-钙黏蛋白阴性与未分化型、淋巴结转移及TNM Ⅲ、Ⅳ期有关(χ~2=11.306,10.208,8.420,P<0.05).结论乙酰肝素酶在胄癌组织中的表达增高,E-钙黏蛋白在胃癌组织中表达降低,乙酰肝素酶与E-钙黏蛋白的异常表达具有协同作用,两者同时异常表达使胃癌细胞具有更强的侵袋转移能力.
목적 탐토을선간소매급E-개점단백대위암침습전이적영향.방법 수집2005년2월지2007년5월요녕성종류의원수술절제적50례위암표본.채용RT-PCR법검측을선간소매mRNA급E-개점단백mRNA재위암조직중적표체,동시응용면역조직화학법검측E-개점단백재위암조직중적표체.계량자료채용방차분석화t검험;계수자료채용χ~2검험.결과 을선간소매여E-개점단백적표체재위암적세포분화정도、림파결전이、TNM분기방면비교,차이유통계학의의(t=1.999,4.258,1.735;1.286,6.794,3.091;χ~2=6.273,9.397,5.640,P<0.05).을선간소매양성/E-개점단백음성여미분화형、림파결전이급TNM Ⅲ、Ⅳ기유관(χ~2=11.306,10.208,8.420,P<0.05).결론 을선간소매재주암조직중적표체증고,E-개점단백재위암조직중표체강저,을선간소매여E-개점단백적이상표체구유협동작용,량자동시이상표체사위암세포구유경강적침대전이능력.
Objective To investigate the effects of heparanase and E-cadherin on the invasion and metastasis of gastric cancer. Methods Fifty specimens of gastric cancer which had been resected at Cancer Hospital of Liaoning Province from February 2005 to May 2007 were collected. The expression of heparanase mRNA and E-cadherin mRNA in these gastric cancer specimens was detected by RT-PCR, and the expression of E-cadherin in these gastric cancer specimens was detected by immunohistochemistry. Data were analyzed by t-test and variance analysis, and the enumeration data analyzed by chi-square test. Results There were significant differences in the expression of heparanase and E-cadherin between gastric cancer cells with high and low differentiation, presence and absence of metastasis, and TNM stages Ⅰ and Ⅱ versus Ⅲ and Ⅳ (t = 1.999, 4.258, 1.735 ; 1.286, 6.794, 3.091; χ~2 =6.273, 9.397, 5.640, P <0.05). The co-expression of heparanase (+) and E-cadherin (-) was correlated with tumor undifferentiation, lymph node metastasis and advanced TNM staging (χ~2 =11.306, 10.208, 8.420, P <0.05). Conclusions Heparanasc shows high expression while E-cadherin shows low expression in gastric cancer tissue. There is a synergistic effect between the abnormal expression of heparanase and E-cadherin, and the gastric cancer cells with coexpression of heparanase and E-cadherin have more malignant potential.