中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2008年
4期
327-332
,共6页
夏英%SUN Quan-fu%尚兵%吕慧敏%冯顺治%程同心%崔宏星%齐雪松%王利平%王浩%孙粉珍%王海军%巨雅洁%毛玲%王琳
夏英%SUN Quan-fu%尚兵%呂慧敏%馮順治%程同心%崔宏星%齊雪鬆%王利平%王浩%孫粉珍%王海軍%巨雅潔%毛玲%王琳
하영%SUN Quan-fu%상병%려혜민%풍순치%정동심%최굉성%제설송%왕리평%왕호%손분진%왕해군%거아길%모령%왕림
肿瘤易感基因%氡暴露%肺癌%CYP4501A0%GSTm1%基因多态性
腫瘤易感基因%氡暴露%肺癌%CYP4501A0%GSTm1%基因多態性
종류역감기인%동폭로%폐암%CYP4501A0%GSTm1%기인다태성
Tumoe susceptibility%Radonexposure%Lung cancer%CYP1A1%GSTm1%Genetic polymorphism
目的 研究高氡暴露地区居民肺癌易感基因CYP4501A1和GSTm1多态性的变化规律,探讨这2种基因多态性与肺癌及环境因素之间的关系.方法 按病例-对照的研究方法从甘肃省庆阳地区选择原发性肺癌病例和相匹配的对照人群进行室内氡、钍射气的测量,并应用PCR-RFLP和PCR方法检测2种基因的多态性.结果 研究表明,携带杂合型CYP1A1(w/m)或缺失型GSTm1(-)基因与野生型基因的个体比较,肺癌发病风险分别为1.46倍(95%CI为0.72～2.95)和1.28倍(95%CI为0.67～2.41).同时携带两种突变基因型的个体,肺癌发病风险为2.00倍(95%CI为0.72～5.58),重度吸烟者肺癌的发病风险会增至2.14倍(95%CI为0.35～13.12).居住在室内氡、钍射气累积有效剂量50～100 mSv并携带突变型CYP1A1(w/m)或GSTm1(-)基因的个体患肺癌的风险分别为2.63倍(95%CI为0.21～31.34)和3.50倍(95%CI为0.31～39.12).有肿瘤家族史的人群肺癌发病风险是3.75倍(95%CI为1.51～9.29),差异有统计学意义.结论 杂合型CYP1A1(w/m)和突变型GSTm1(-)基因是较重要的肺癌风险因子,尤其在有效剂量50～100 mSy和2种突变基因的协同作用时.
目的 研究高氡暴露地區居民肺癌易感基因CYP4501A1和GSTm1多態性的變化規律,探討這2種基因多態性與肺癌及環境因素之間的關繫.方法 按病例-對照的研究方法從甘肅省慶暘地區選擇原髮性肺癌病例和相匹配的對照人群進行室內氡、釷射氣的測量,併應用PCR-RFLP和PCR方法檢測2種基因的多態性.結果 研究錶明,攜帶雜閤型CYP1A1(w/m)或缺失型GSTm1(-)基因與野生型基因的箇體比較,肺癌髮病風險分彆為1.46倍(95%CI為0.72～2.95)和1.28倍(95%CI為0.67～2.41).同時攜帶兩種突變基因型的箇體,肺癌髮病風險為2.00倍(95%CI為0.72～5.58),重度吸煙者肺癌的髮病風險會增至2.14倍(95%CI為0.35～13.12).居住在室內氡、釷射氣纍積有效劑量50～100 mSv併攜帶突變型CYP1A1(w/m)或GSTm1(-)基因的箇體患肺癌的風險分彆為2.63倍(95%CI為0.21～31.34)和3.50倍(95%CI為0.31～39.12).有腫瘤傢族史的人群肺癌髮病風險是3.75倍(95%CI為1.51～9.29),差異有統計學意義.結論 雜閤型CYP1A1(w/m)和突變型GSTm1(-)基因是較重要的肺癌風險因子,尤其在有效劑量50～100 mSy和2種突變基因的協同作用時.
목적 연구고동폭로지구거민폐암역감기인CYP4501A1화GSTm1다태성적변화규률,탐토저2충기인다태성여폐암급배경인소지간적관계.방법 안병례-대조적연구방법종감숙성경양지구선택원발성폐암병례화상필배적대조인군진행실내동、토사기적측량,병응용PCR-RFLP화PCR방법검측2충기인적다태성.결과 연구표명,휴대잡합형CYP1A1(w/m)혹결실형GSTm1(-)기인여야생형기인적개체비교,폐암발병풍험분별위1.46배(95%CI위0.72～2.95)화1.28배(95%CI위0.67～2.41).동시휴대량충돌변기인형적개체,폐암발병풍험위2.00배(95%CI위0.72～5.58),중도흡연자폐암적발병풍험회증지2.14배(95%CI위0.35～13.12).거주재실내동、토사기루적유효제량50～100 mSv병휴대돌변형CYP1A1(w/m)혹GSTm1(-)기인적개체환폐암적풍험분별위2.63배(95%CI위0.21～31.34)화3.50배(95%CI위0.31～39.12).유종류가족사적인군폐암발병풍험시3.75배(95%CI위1.51～9.29),차이유통계학의의.결론 잡합형CYP1A1(w/m)화돌변형GSTm1(-)기인시교중요적폐암풍험인자,우기재유효제량50～100 mSy화2충돌변기인적협동작용시.
Objective To investigate whether the CYP1A1 and GSTm1 genetic polymorphism are agsoaiate with lung cancer in the residents of high radon exposure area.Methods A case-control method was performed,lung cancer and control individials were selected from Qingyang county of Gansu Province.The indoor radon and thoron concentration were measured.CYP1A1 and GSTm1 genetic polymorphism were detected by PCR-RFLP or multiplex-PCR.Results The risk of developing lung cancer for people carried with heterozygous CYP1A1(w/m)and mutation genotype GSTm1(-)were 1.46(95%CI 0.72-2.95)and 1.28(95%CI 0.67-2.41)higher than wide type.respectively.The risk of lung carccr for combination of CYP1A1(w/m)and GSTm1(-)increased to 2.00 fold(95%CI 0.72-5.58),the heavy smokers who carried with heterozygous of CYP1A1(w/m)wag 2.14fold(95%CI 0.35～13.12).People who carried with heterozygous CYP1A1(w/m)or GSTm1(-)when effective dose was in 50-100 mSv,lung canser risk will fised to 2.63 fold(95%CI 0.21～31.34)and 3.50 fold(95%CI 0.31-39.12).The risk of lung cancer for people from tumors family was 3.75 fold(95%CI 1.51-9.29)with statistic significance.Conclusions The CYP1A1(w/m)and GSTm1(-)are two more risk factors for lung carcer,specifically when effective dose Wag in 50-100 mSv and the two gene functions combined together.
