中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2012年
1期
50-52
,共3页
尤海燕%李晶%焦志军%汪毅%裘影影%吴玲%郑东海
尤海燕%李晶%焦誌軍%汪毅%裘影影%吳玲%鄭東海
우해연%리정%초지군%왕의%구영영%오령%정동해
红斑狼疮,系统性%T淋巴细胞,辅助诱导%T淋巴细胞
紅斑狼瘡,繫統性%T淋巴細胞,輔助誘導%T淋巴細胞
홍반랑창,계통성%T림파세포,보조유도%T림파세포
Lupus erythematosus,systemic%T-lymphocytes,helper-inducer%T-lymphocytes
目的 探讨外周血辅助性T细胞17(Th17)与调节性T细胞在系统性红斑狼疮(SLE)发生、发展中的变化.方法 选取32例SLE活动期患者作为SLE活动组、30例SLE稳定期患者作为SLE稳定组及25名健康者作为对照组,采用流式细胞术及定量聚合酶链反应(PCR)的方法,分别从蛋白质水平与mRNA水平检测其外周血CD4+T细胞IL-17及FoxP3的表达.采用单因素方差分析进行统计学处理.结果 SLE活动组外周血CD4+T细胞IL-17蛋白质(1.01±0.22)％及mRNA(2.04±0.63)表达水平显著高于SLE稳定组(0.48±0.16)％、(1.12±0.34)及健康对照组(0.41±0.12)％、1(P＜0.01),但SLE稳定组与健康者对照组差异无统计学意义(P＞0.05).SLE活动组外周血CD4+T细胞FoxP3蛋白质(2.36±0.54)％及mRNA(0.42±0.16)表达水平显著低于SLE稳定组(4.34±0.95)％、(0.87±0.28)及健康对照组(5.09±1.17)％、 1(P＜0.01),SLE稳定组又低于健康对照组(P＜0.05).结论 SLE患者外周血中可能存在Th17/调节性T细胞的失衡,且失衡程度可能与病情活动性相关.
目的 探討外週血輔助性T細胞17(Th17)與調節性T細胞在繫統性紅斑狼瘡(SLE)髮生、髮展中的變化.方法 選取32例SLE活動期患者作為SLE活動組、30例SLE穩定期患者作為SLE穩定組及25名健康者作為對照組,採用流式細胞術及定量聚閤酶鏈反應(PCR)的方法,分彆從蛋白質水平與mRNA水平檢測其外週血CD4+T細胞IL-17及FoxP3的錶達.採用單因素方差分析進行統計學處理.結果 SLE活動組外週血CD4+T細胞IL-17蛋白質(1.01±0.22)％及mRNA(2.04±0.63)錶達水平顯著高于SLE穩定組(0.48±0.16)％、(1.12±0.34)及健康對照組(0.41±0.12)％、1(P＜0.01),但SLE穩定組與健康者對照組差異無統計學意義(P＞0.05).SLE活動組外週血CD4+T細胞FoxP3蛋白質(2.36±0.54)％及mRNA(0.42±0.16)錶達水平顯著低于SLE穩定組(4.34±0.95)％、(0.87±0.28)及健康對照組(5.09±1.17)％、 1(P＜0.01),SLE穩定組又低于健康對照組(P＜0.05).結論 SLE患者外週血中可能存在Th17/調節性T細胞的失衡,且失衡程度可能與病情活動性相關.
목적 탐토외주혈보조성T세포17(Th17)여조절성T세포재계통성홍반랑창(SLE)발생、발전중적변화.방법 선취32례SLE활동기환자작위SLE활동조、30례SLE은정기환자작위SLE은정조급25명건강자작위대조조,채용류식세포술급정량취합매련반응(PCR)적방법,분별종단백질수평여mRNA수평검측기외주혈CD4+T세포IL-17급FoxP3적표체.채용단인소방차분석진행통계학처리.결과 SLE활동조외주혈CD4+T세포IL-17단백질(1.01±0.22)％급mRNA(2.04±0.63)표체수평현저고우SLE은정조(0.48±0.16)％、(1.12±0.34)급건강대조조(0.41±0.12)％、1(P＜0.01),단SLE은정조여건강자대조조차이무통계학의의(P＞0.05).SLE활동조외주혈CD4+T세포FoxP3단백질(2.36±0.54)％급mRNA(0.42±0.16)표체수평현저저우SLE은정조(4.34±0.95)％、(0.87±0.28)급건강대조조(5.09±1.17)％、 1(P＜0.01),SLE은정조우저우건강대조조(P＜0.05).결론 SLE환자외주혈중가능존재Th17/조절성T세포적실형,차실형정도가능여병정활동성상관.
Objective To investigate the profile of Th17/Treg balance in the peripheral blood of patients with systemic lupus erythematosus (SLE).Methods Thirty-two SLE patients in active disease were selected and 30 SLE patients in remission were included in this study.The control group was consisted of 25healthy individuals.The expressions of IL-17 and FoxP3 on CD4+ T cells in the peripheral blood were assessed by flow cytometry and the mRNA levels of these two cytokines were examined by quantitative PCR respectively.ANOVA was used for statistical analysis.Results The percentage of CD4+IL-17+ T cells and IL-17mRNA expression of the active group were significantly higher than those of the remission group and control group[(l.0l±0.22)％,(2.04±0.63)vs (0.48±0.16)％,(1.12±0.34) vs (0.41±0.12)％,1; P＜0.01].There was no difference between the remission group and control group (P＞0.05).However,the percentage of CI4+FoxP3 + T cells and FoxP3 mRNA expression of the active group were significantly lower than those of the remission group and control group [(2.36±t0.54)％,(0.42±0.16) vs (4.34±0.95)％,(0.87±t0.28) vs (5.09±11.17)％,1; P＜0.01 ],and there was also significant differences between the remission group and control group (P＜0.05).Conclusion Thl7/Treg balance shift may exist in the peripheral blood of patients with SLE and the degree of imbalance may be related to disease activity of SLE.
