CAJ | 학술논문

目的观察甘油致小鼠急性肾脏损伤后肾脏修复的情况.方法肌肉注射甘油致小鼠发生急性肾脏损伤后出取肾脏组织,于损伤后的不同时间点进行肾脏组织形态学检测;同时,应用免疫组织化学技术检测核增殖抗原在肾脏组织的表达情况,分析肾脏损伤后的修复情况.结果肌肉注射甘油3 d后小鼠开始出现急性肾脏损害的表现.主要表现为肾脏肿胀,肾脏指数增加,实验组与对照组相比t=2.262,P<0.05,差异有统计学意义,肾小管腔内出现大量蛋白质样物质沉积,肾小管上皮细胞变性,坏死.于肌肉注射甘油3 d后,免疫组织化学技术检测显示,小鼠损伤肾脏的肾小管出现大量核增殖抗原表达阳性的再生小管上皮细胞.结论肾小管损伤后,可以出现肾小管上皮细胞增殖进行损伤的修复.
목적 관찰감유치소서급성신장손상후신장수복적정황.방법 기육주사감유치소서발생급성신장손상후출취신장조직,우손상후적불동시간점진행신장조직형태학검측;동시,응용면역조직화학기술검측핵증식항원재신장조직적표체정황,분석신장손상후적수복정황.결과 기육주사감유3 d후소서개시출현급성신장손해적표현.주요표현위신장종창,신장지수증가,실험조여대조조상비t=2.262,P<0.05,차이유통계학의의,신소관강내출현대량단백질양물질침적,신소관상피세포변성,배사.우기육주사감유3 d후,면역조직화학기술검측현시,소서손상신장적신소관출현대량핵증식항원표체양성적재생소관상피세포.결론 신소관손상후,가이출현신소관상피세포증식진행손상적수복.
Objective To observe the renal regeneration after acute renal injury established by glycerin administration in mice. Methods The mouse model was established by glycerin administration. Histological e-valuation was performed via routine methods. Dynamic regeneration of renal was accessed by detecting proliferating cell nuclear antigen using immunohistochemical staining. Results At 3th day after glycerin administration , kidney tissues were collected and evaluated. Kidney swelled and renal index increased (compaired to control mice,t=2. 262 ,P<0.05). Renal tubules were filled with proteins mass. Renal tubele cells suffered with degeneration, necrosis and desquamation could be found. Renal regeneration in tubeles was detected by measuring proliferating cell nuclear antigen. Conclusions Renal regeneration originates from residual tubular cells after renal injury in mice model.