中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2009年
6期
410-412
,共3页
杜杰%江涛%王西墨%张姝翌
杜傑%江濤%王西墨%張姝翌
두걸%강도%왕서묵%장주익
胰腺肿瘤%趋化因子类%细胞增殖%细胞黏附%细胞侵袭%细胞迁移
胰腺腫瘤%趨化因子類%細胞增殖%細胞黏附%細胞侵襲%細胞遷移
이선종류%추화인자류%세포증식%세포점부%세포침습%세포천이
Pancreatic neoplasms%Chemotactic factors%Cell proliferation%Cell adhesion%Cell invasion%Cell migration
目的 探讨趋化因子配体16(CXCL16)对人胰腺癌细胞PANC1生物学行为的影响.方法 取对数生长期的人胰腺癌细胞PANC1,分别加人50、100、200 ng/ml的重组人CXCL16(rhCXCL16)和抗CXCL16 抗体处理4 h,以不加rhCLCL16作为对照.采用MTT法检测细胞增殖,采用Mqrtigel基质检测细胞的黏附率,采用Transwell小室检测细胞侵袭和迁移能力.结果 100 ng/ml rhCXCL16处理PANC1细胞后,细胞增殖、对基质的黏附率、侵袭和迁移能力分别为0.264±0.021、(91.4±8.6)%、1.246±0.216、1.361±0.276,其中细胞对基质的黏附率、侵袭和迁移能力均显著高于对照组的(20.6±3.2)%、0.259±0.013、0.199±0.1308(P<0.01),对细胞的增殖不影响;200ng/ml rhCXCL16处理后,细胞对基质的黏附率、侵袭和迁移能力进一步提高到(92.1±6.3)%、1.511±0.174、1.600±0.20s(P<0.05).结论 CXCL16可诱导人胰腺癌细胞PANC1增强对基质的黏附性、侵袭性和迁移性.
目的 探討趨化因子配體16(CXCL16)對人胰腺癌細胞PANC1生物學行為的影響.方法 取對數生長期的人胰腺癌細胞PANC1,分彆加人50、100、200 ng/ml的重組人CXCL16(rhCXCL16)和抗CXCL16 抗體處理4 h,以不加rhCLCL16作為對照.採用MTT法檢測細胞增殖,採用Mqrtigel基質檢測細胞的黏附率,採用Transwell小室檢測細胞侵襲和遷移能力.結果 100 ng/ml rhCXCL16處理PANC1細胞後,細胞增殖、對基質的黏附率、侵襲和遷移能力分彆為0.264±0.021、(91.4±8.6)%、1.246±0.216、1.361±0.276,其中細胞對基質的黏附率、侵襲和遷移能力均顯著高于對照組的(20.6±3.2)%、0.259±0.013、0.199±0.1308(P<0.01),對細胞的增殖不影響;200ng/ml rhCXCL16處理後,細胞對基質的黏附率、侵襲和遷移能力進一步提高到(92.1±6.3)%、1.511±0.174、1.600±0.20s(P<0.05).結論 CXCL16可誘導人胰腺癌細胞PANC1增彊對基質的黏附性、侵襲性和遷移性.
목적 탐토추화인자배체16(CXCL16)대인이선암세포PANC1생물학행위적영향.방법 취대수생장기적인이선암세포PANC1,분별가인50、100、200 ng/ml적중조인CXCL16(rhCXCL16)화항CXCL16 항체처리4 h,이불가rhCLCL16작위대조.채용MTT법검측세포증식,채용Mqrtigel기질검측세포적점부솔,채용Transwell소실검측세포침습화천이능력.결과 100 ng/ml rhCXCL16처리PANC1세포후,세포증식、대기질적점부솔、침습화천이능력분별위0.264±0.021、(91.4±8.6)%、1.246±0.216、1.361±0.276,기중세포대기질적점부솔、침습화천이능력균현저고우대조조적(20.6±3.2)%、0.259±0.013、0.199±0.1308(P<0.01),대세포적증식불영향;200ng/ml rhCXCL16처리후,세포대기질적점부솔、침습화천이능력진일보제고도(92.1±6.3)%、1.511±0.174、1.600±0.20s(P<0.05).결론 CXCL16가유도인이선암세포PANC1증강대기질적점부성、침습성화천이성.
Objective To investigate the effect of CXCL16 on the biological behavior of human panereatic cancel cells PANC1.Methods Exponentially growing PANCI cells was exposed exposed to different concentration of rhCXCL16(50,100,200 mg/ml)and CXCL16 antibody for4 h,and PANC1 without rhCLCL16 Ireatment was used as the control.The proliferation was determined by MTT method,adhesion rate was determined by Mqrtigel matrix,invasion and migration of PANC1 cells were assayed by Transwell chamber.Results After 100 ng/ml rhCXCL16 treatment,proliferation,adhesion rate,invasion and migration of PANC1 eels were 0.264±0.021.991.4± 8.6)%,1.246±0.216,1.361± 0.276,respectively;and the adhesion rate.invasion and migration were significantly higher than(20.6±3.2)%,0.259±0.013,0.199±0.008 in the control group(P<0.01).and without significant effect on proliferation.After 200 ng/ml rhCXCL16 treatment,proliferation,adhesion rate,invasion and migration of PANC1 cells were further improved.Conclusions rhCXCL16 could enhance the ability of adhesion,invasion and migration of PANC1 cells.
