CAJ | 학술논문

目的研究弥漫性轴索损伤后大鼠丘脑组织中脑红蛋白(NGB)mRNA的表达变化,初步探讨NGB与颅脑损伤的关系.方法选择改进的Mamarou法制作弥漫性轴索损伤模型,采用RT-PCR检测颅脑损伤后不同时间丘脑组织中NGB mRNA的表达情况,并对所得数据进行分析.结果在伤后30 min脑组织中NGB mRNA的表达明显升高,而24 h时则有所下降,48 h又上升达高峰.结论弥漫性颅脑损伤后,丘脑组织中NGB mRNA表达升高,提示其可能参与神经元损伤后应激及继发缺血、缺氧性脑损伤的应答机制.
목적 연구미만성축색손상후대서구뇌조직중뇌홍단백(NGB)mRNA적표체변화,초보탐토NGB여로뇌손상적관계.방법 선택개진적Mamarou법제작미만성축색손상모형,채용RT-PCR검측로뇌손상후불동시간구뇌조직중NGB mRNA적표체정황,병대소득수거진행분석.결과 재상후30 min뇌조직중NGB mRNA적표체명현승고,이24 h시칙유소하강,48 h우상승체고봉.결론 미만성로뇌손상후,구뇌조직중NGB mRNA표체승고,제시기가능삼여신경원손상후응격급계발결혈、결양성뇌손상적응답궤제.
Objective To study mRNA expression of neuroglobin (NGB) in thalamus of DAI rats and explore the relation between NGB and heed injury. Method 46 male SD rats were randomly divided into four groups. The DAI model was established according to modi-fied Mamamu method. The mRNA expression of NGB in thalamus at different time point after injury was detected by RT-PCR method. All data were analyzed with statistical software. Result The mRNA expression of NGB in thalamus increased obviously from 30 minutes after in-jury while decreased at the 24th hour after injury. But the expression increased again and reached peak at the 48th hour after injury. Conclu-sion The mRNA expression of NGB in thalamus increased after DM. Those changes suggested NGB might participate in the response mech-anism of stress, secondary ischemia and hypoxic brain injury after neuron injury.