中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2009年
3期
161-163
,共3页
高压氧%缺氧缺血性脑损伤%脑组织存活素%细胞凋亡蛋白酶-3%凋亡
高壓氧%缺氧缺血性腦損傷%腦組織存活素%細胞凋亡蛋白酶-3%凋亡
고압양%결양결혈성뇌손상%뇌조직존활소%세포조망단백매-3%조망
Hyperbaric oxygen(HBO)%Hypoxia-ischemia brain damage (HIBD)%Survivin%Caspase-3%Apoptosis
目的 观察高压氧(HBO)对新生大鼠缺氧缺血性脑损伤(hypoxia-ischemia brain damage,HIBD)后脑组织存活素(survivin)及细胞凋亡蛋白酶-3(caspase-3)表达的影响,并探讨其保护机制.方法 144只7 d龄SD大鼠随机分为对照组(n=16)、新生HIBD组(n=64)及HBO治疗组(n=64).采用经典的Rice法制作缺氧缺血性脑损伤模型,应用Epics XL流式细胞仪检测细胞凋亡情况,并应用免疫组织化学法检测Survivin和Caspase-3表达变化.结果 与对照组相比,HIBD组细胞凋亡率及Survivin、Caspase-3表达水平明显升高,组间比较差异有统计学意义(P<0.05).通过相关性分析,HBO治疗可促进存活素表达,其表达水平和caspase-3表达负相关.结论 HBO可能通过增加抗凋亡因子Survivin表达来抑制Caspase-3激活,减少缺氧缺血性脑损伤后细胞凋亡,发挥神经保护作用.
目的 觀察高壓氧(HBO)對新生大鼠缺氧缺血性腦損傷(hypoxia-ischemia brain damage,HIBD)後腦組織存活素(survivin)及細胞凋亡蛋白酶-3(caspase-3)錶達的影響,併探討其保護機製.方法 144隻7 d齡SD大鼠隨機分為對照組(n=16)、新生HIBD組(n=64)及HBO治療組(n=64).採用經典的Rice法製作缺氧缺血性腦損傷模型,應用Epics XL流式細胞儀檢測細胞凋亡情況,併應用免疫組織化學法檢測Survivin和Caspase-3錶達變化.結果 與對照組相比,HIBD組細胞凋亡率及Survivin、Caspase-3錶達水平明顯升高,組間比較差異有統計學意義(P<0.05).通過相關性分析,HBO治療可促進存活素錶達,其錶達水平和caspase-3錶達負相關.結論 HBO可能通過增加抗凋亡因子Survivin錶達來抑製Caspase-3激活,減少缺氧缺血性腦損傷後細胞凋亡,髮揮神經保護作用.
목적 관찰고압양(HBO)대신생대서결양결혈성뇌손상(hypoxia-ischemia brain damage,HIBD)후뇌조직존활소(survivin)급세포조망단백매-3(caspase-3)표체적영향,병탐토기보호궤제.방법 144지7 d령SD대서수궤분위대조조(n=16)、신생HIBD조(n=64)급HBO치료조(n=64).채용경전적Rice법제작결양결혈성뇌손상모형,응용Epics XL류식세포의검측세포조망정황,병응용면역조직화학법검측Survivin화Caspase-3표체변화.결과 여대조조상비,HIBD조세포조망솔급Survivin、Caspase-3표체수평명현승고,조간비교차이유통계학의의(P<0.05).통과상관성분석,HBO치료가촉진존활소표체,기표체수평화caspase-3표체부상관.결론 HBO가능통과증가항조망인자Survivin표체래억제Caspase-3격활,감소결양결혈성뇌손상후세포조망,발휘신경보호작용.
Objective To explore the effect of hyperbaric oxygen (HBO) on the expression of survivin and caspase-3 following hypoxia-ischemia brain damage (HIBD) in neonatal rats,and the mechanism involved in the protection of the neural system by HBO.Methods One hundred and forty-four ( aged seven days) rats were randomly divided into three groups,i.e.the control group (n = 16),the HIBD group (n =64) and the HBO group (n =64).An experimental HIBD model was developed by the Rice method.Following damage,samples were obtained for measuring apoptosis of cells with the Epics XL flow cytometer and survivin expression and caspase-3 proteins were measured with immuno-histochemistry.Results When compared with that of the control group,the number of apoptotic cells,survivin,and caspase-3 immunopositive cells all increased significantly in the HBID group,and statistical differences could be seen between groups ( P < 0.05 ).Analyses showed that HBO could significantly enhance the expression of survivin,with negative correlation between the level of survivin expression and the activity of caspase-3.Conclusions Increased expression of survivin by HBO might be one of the mechanisms involved in the regulation of caspase-3 and inhibition of neural cell apoptosis in cortex,which might be protective to neurons.
