中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2009年
7期
473-476
,共4页
顾宁琰%陈敏%崔盘根%周武庆%顾恒
顧寧琰%陳敏%崔盤根%週武慶%顧恆
고저염%진민%최반근%주무경%고항
银屑病%白细胞,单核%受体,干扰素%受体,肿瘤坏死因子
銀屑病%白細胞,單覈%受體,榦擾素%受體,腫瘤壞死因子
은설병%백세포,단핵%수체,간우소%수체,종류배사인자
Psoriasis%Leukocytes,mononuclear%Receptors,interferon%Receptors,tumor necrosis factor
目的 探讨寻常性银屑病患者外周血单一核细胞IFN-γ受体mRNA和TNF-α仅受体mRNA的表达及其在银屑病发病机制中的作用.方法 采集50例寻常性银屑病患者外周静脉血,分离单一核细胞后,以RT-PCR法检测其IFN-γ受体mRNA和TNF-α受体mRNA的表达,PASI评分法评估银屑病的严重程度.以24例正常人作对照.结果 50例银屑病患者外周血单一核细胞IFN-γ受体mRNA的表达均值为1.11±0.55,其中37例进行期患者为1.13±0.57,13例静止期患者为1.03±0.52,正常人对照组为0.72±0.17,银屑病患者显著高于正常人对照组(P=0.0034),进行期显著高于正常人对照组(P=0.008),而静止期与正常人对照组差异无统计学意义.正常人对照组和银屑病患者外周血单一核细胞TNF-α受体mRNA的表达水平差异无统计学意义.银屑病患者外周血单一核细胞IFN-γ受体mRNA表达水平与TNF-α受体mRNA表达水平及PASI值均无相关性.结论 IFN-γ受体mRNA在寻常性银屑病患者外周血单一核细胞中异常高表达,但与疾病的活动性无关.
目的 探討尋常性銀屑病患者外週血單一覈細胞IFN-γ受體mRNA和TNF-α僅受體mRNA的錶達及其在銀屑病髮病機製中的作用.方法 採集50例尋常性銀屑病患者外週靜脈血,分離單一覈細胞後,以RT-PCR法檢測其IFN-γ受體mRNA和TNF-α受體mRNA的錶達,PASI評分法評估銀屑病的嚴重程度.以24例正常人作對照.結果 50例銀屑病患者外週血單一覈細胞IFN-γ受體mRNA的錶達均值為1.11±0.55,其中37例進行期患者為1.13±0.57,13例靜止期患者為1.03±0.52,正常人對照組為0.72±0.17,銀屑病患者顯著高于正常人對照組(P=0.0034),進行期顯著高于正常人對照組(P=0.008),而靜止期與正常人對照組差異無統計學意義.正常人對照組和銀屑病患者外週血單一覈細胞TNF-α受體mRNA的錶達水平差異無統計學意義.銀屑病患者外週血單一覈細胞IFN-γ受體mRNA錶達水平與TNF-α受體mRNA錶達水平及PASI值均無相關性.結論 IFN-γ受體mRNA在尋常性銀屑病患者外週血單一覈細胞中異常高錶達,但與疾病的活動性無關.
목적 탐토심상성은설병환자외주혈단일핵세포IFN-γ수체mRNA화TNF-α부수체mRNA적표체급기재은설병발병궤제중적작용.방법 채집50례심상성은설병환자외주정맥혈,분리단일핵세포후,이RT-PCR법검측기IFN-γ수체mRNA화TNF-α수체mRNA적표체,PASI평분법평고은설병적엄중정도.이24례정상인작대조.결과 50례은설병환자외주혈단일핵세포IFN-γ수체mRNA적표체균치위1.11±0.55,기중37례진행기환자위1.13±0.57,13례정지기환자위1.03±0.52,정상인대조조위0.72±0.17,은설병환자현저고우정상인대조조(P=0.0034),진행기현저고우정상인대조조(P=0.008),이정지기여정상인대조조차이무통계학의의.정상인대조조화은설병환자외주혈단일핵세포TNF-α수체mRNA적표체수평차이무통계학의의.은설병환자외주혈단일핵세포IFN-γ수체mRNA표체수평여TNF-α수체mRNA표체수평급PASI치균무상관성.결론 IFN-γ수체mRNA재심상성은설병환자외주혈단일핵세포중이상고표체,단여질병적활동성무관.
Objective To investigate the mRNA expressions of IFN-gamma receptor and TNF-alpha receptor in peripheral blood mononuclear cells (PBMCs) of patients with psoriasis vulgaris and their role in the pathogenesis of psoriasis. Methods Fifty patients with psoriasis vulgaris (37 cases of active psoriasis and 13 cases of stable psoriasis) and 24 healthy human controls were included in this study. PBMCs were isolated from blood samples obtained from all patients and controls. The mRNA expressions of IFN-gamma receptor and TNF-aipha receptor in PBMCs were detected by RT-PCR. The disease severity in patients was evaluated by psoriasis area and severity index (PASI). Results The mRNA expressions of IFN-gamma receptor and TNF-alpha receptor were observed in the PBMCs of all subjects. The mRNA expression levels of IFN-gamma receptor were 0.72 ± 0.17 in healthy controls, 1.11 ± 0.55 in all patients with psoriasis, 1.13 ±0.57 in patients with active psoriasis and 1.03 ± 0.52 in patients with stable psoriasis, respectively. A signifi-cant increase was observed in the expression levels of IFN-gamma receptor mRNA in all psoriatic patients and in patients with active psoriasis compared with those in healthy controls (both P < 0.05), but there was no significant difference between the healthy controls and patients with stable psoriasis (P ＞ 0.05). The expres-sion levels of TNF-alpha receptor mRNA were 2.05 ± 1.34 in healthy controls, 2.70 ± 3.80 in all psoriatic patients, 2.90 ± 4.40 in patients with active psoriasis, 2.14 ± 1.05 in patients with stable psoriasis, respectively;there was no significant difference between psoriatic patients and healthy controls (P ＞ 0.05). However, no correlation was found between the mRNA expression of IFN-gamma receptor, that of TNF-alpha receptor,and disease severity in psoriatic patients. Conclusions The mRNA expression of IFN-gamma receptor in PBMCs is up-regulated in patients with psoriasis vulgaris, which is unrelated to the activity of psoriasis.
