中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2011年
8期
741-745
,共5页
屈振亮%崔乃强%席兆华%杜智
屈振亮%崔迺彊%席兆華%杜智
굴진량%최내강%석조화%두지
肝炎病毒,乙型%胆道肿瘤%病毒整合
肝炎病毒,乙型%膽道腫瘤%病毒整閤
간염병독,을형%담도종류%병독정합
Hepatitis B virus%Biliary tract neoplasms%Virus integration
目的 探索肝门部胆管癌组织内有无乙型肝炎病毒(HBV)基因的整合,并鉴定其在细胞染色体上的整合位点.方法 收集10例肝门部胆管癌新鲜组织标本,并以同期1例因十二指肠乳头癌而行胰十二指肠切除术患者的正常肝门部胆管组织标本作为对照.抽提组织DNA,采用HBV-Alu-PCR方法扩增HBV X基因及其侧翼的人基因组片段.PCR产物经序列测定后,应用序列分析软件分析比较HBV X基因序列,对其上、下游序列进行Entrez BLAST和NCBI MapViewer分析,确定HBV整合在染色体上的精确位置.结果 10例肝门部胆管癌组织中,有5例可检测到HBV整合片段,共获得6个整合位点.序列分析发现HBV X基因均以截断形式插入到宿主细胞基因组,其中CpG结合蛋白基因上游被HBV重复整合,与肿瘤发生密切相关的p53基因亦被HBV整合.结论 肝门部胆管癌组织内有较高的HBV整合率,HBV常整合在与肿瘤发生相关的基因中,HBV感染与肝门部胆管癌发生有关.
目的 探索肝門部膽管癌組織內有無乙型肝炎病毒(HBV)基因的整閤,併鑒定其在細胞染色體上的整閤位點.方法 收集10例肝門部膽管癌新鮮組織標本,併以同期1例因十二指腸乳頭癌而行胰十二指腸切除術患者的正常肝門部膽管組織標本作為對照.抽提組織DNA,採用HBV-Alu-PCR方法擴增HBV X基因及其側翼的人基因組片段.PCR產物經序列測定後,應用序列分析軟件分析比較HBV X基因序列,對其上、下遊序列進行Entrez BLAST和NCBI MapViewer分析,確定HBV整閤在染色體上的精確位置.結果 10例肝門部膽管癌組織中,有5例可檢測到HBV整閤片段,共穫得6箇整閤位點.序列分析髮現HBV X基因均以截斷形式插入到宿主細胞基因組,其中CpG結閤蛋白基因上遊被HBV重複整閤,與腫瘤髮生密切相關的p53基因亦被HBV整閤.結論 肝門部膽管癌組織內有較高的HBV整閤率,HBV常整閤在與腫瘤髮生相關的基因中,HBV感染與肝門部膽管癌髮生有關.
목적 탐색간문부담관암조직내유무을형간염병독(HBV)기인적정합,병감정기재세포염색체상적정합위점.방법 수집10례간문부담관암신선조직표본,병이동기1례인십이지장유두암이행이십이지장절제술환자적정상간문부담관조직표본작위대조.추제조직DNA,채용HBV-Alu-PCR방법확증HBV X기인급기측익적인기인조편단.PCR산물경서렬측정후,응용서렬분석연건분석비교HBV X기인서렬,대기상、하유서렬진행Entrez BLAST화NCBI MapViewer분석,학정HBV정합재염색체상적정학위치.결과 10례간문부담관암조직중,유5례가검측도HBV정합편단,공획득6개정합위점.서렬분석발현HBV X기인균이절단형식삽입도숙주세포기인조,기중CpG결합단백기인상유피HBV중복정합,여종류발생밀절상관적p53기인역피HBV정합.결론 간문부담관암조직내유교고적HBV정합솔,HBV상정합재여종류발생상관적기인중,HBV감염여간문부담관암발생유관.
Objectives To study the phenomena of hepatitis B virus (HBV) integration into the tissues of hilar cholangiocarcinoma (HCCA) and to identify the integration sites in the host genome.Methods Ten fresh HCCA samples were collected from the tissues by surgical ablation, 1 normal hilar bile duct sample selected as control.Cellular DNA were extracted by Wizard(R) SV Genomic DNA Purification System.PCR-derived assay(HBV-Alu-PCR) was employed to amplify the viral-host junctions which contain the HBV sequence and the adjacent cellular flanking sequences.The PCR products were purified and subjected to sequencing by ABI-3730XL Auto DNA Analyzer.The sequence analysis of viral-host junctions was performed by DNASIS(R) MAX 3.0 bioinformatics software.The insertion sites between viral and cellular sequences were identified through homology comparison using NCBI BLAST and MapViewer search.Results In 10 HCCA samples,5 were demonstrated to have HBV integration fragments with total 6 inserted sites identified.Sequence analysis from viral-host junction showed that HBV X gene inserted into host genome at random distribution with truncated fragments.HBV integration recurrently targeted the unknown region in upstream of CXXC finger protein-1 (CpG-binding protein) gene (4 cases).p53 tumor suppressor gene was also found at the integration site.Conclusions There is high integration rate of HBV DNA into cellular genome of HCCA.HBV integration is found frequently into or close to cancer-related genes.The findings demonstrat that HBV infection might have association with the pathogenesis of HCCA.
