CAJ | 학술논문

目的通过观察糖尿病大鼠肾小动脉内膜/中膜厚度比变化,初步探讨其与炎性因子的相关关系及对糖尿病肾病(DN)血管病变的影响.方法 70只健康大鼠被随机分为DN组(n=40)和正常对照组(N,n=30).DN大鼠模型采用链脲菌素(STZ)腹腔注射诱导,成功35只,N组子同等剂量柠檬酸缓冲液.分别于4、12、24周处死动物,取大鼠股静脉血查生化指标.用免疫荧光方法检测肾小动脉内膜/中膜厚度比.用免疫组化及原位杂交方法检测各时间点肾小动脉中单核细胞趋化因子1(MCP-1)的蛋白和mRNA表达变化.结果生化结果显示DN组各时间点血糖及尿蛋白量(24 h)均显著高于N组(P＜0.05),自第12周开始,DN组血肌酐、BUN、血磷显著高于N组(P＜0.05).免疫组化及原位杂交显示DN组肾小动脉4周时已有MCP-1蛋白及mRNA表达,随时间延长表达逐渐增强,24周表达最强,各时间点均显著高于N组(均P＜0.05).免疫荧光结果显示,第4周时DN组内膜/中膜厚度比与N组差异无统计学意义,12周时高于N组(P＞0.05),24周时显著高于N组(P＜0.05).DN组小动脉内膜/中膜厚度比与MCP-1表达、胆固醇、三酰甘油、血磷水平均呈正相关(r=0.742,P＜0.01;r=0.740,P＜0.01;r=0.829,P＜0.01;r=0.580,P＜0.01).结论 DN早期小动脉内膜/中膜厚度比与MCP-1呈正相关.MCP-1可能参与了DN血管病变.
목적 통과관찰당뇨병대서신소동맥내막/중막후도비변화,초보탐토기여염성인자적상관관계급대당뇨병신병(DN)혈관병변적영향.방법 70지건강대서피수궤분위DN조(n=40)화정상대조조(N,n=30).DN대서모형채용련뇨균소(STZ)복강주사유도,성공35지,N조자동등제량저몽산완충액.분별우4、12、24주처사동물,취대서고정맥혈사생화지표.용면역형광방법검측신소동맥내막/중막후도비.용면역조화급원위잡교방법검측각시간점신소동맥중단핵세포추화인자1(MCP-1)적단백화mRNA표체변화.결과 생화결과현시DN조각시간점혈당급뇨단백량(24 h)균현저고우N조(P＜0.05),자제12주개시,DN조혈기항、BUN、혈린현저고우N조(P＜0.05).면역조화급원위잡교현시DN조신소동맥4주시이유MCP-1단백급mRNA표체,수시간연장표체축점증강,24주표체최강,각시간점균현저고우N조(균P＜0.05).면역형광결과현시,제4주시DN조내막/중막후도비여N조차이무통계학의의,12주시고우N조(P＞0.05),24주시현저고우N조(P＜0.05).DN조소동맥내막/중막후도비여MCP-1표체、담고순、삼선감유、혈린수평균정정상관(r=0.742,P＜0.01;r=0.740,P＜0.01;r=0.829,P＜0.01;r=0.580,P＜0.01).결론 DN조기소동맥내막/중막후도비여MCP-1정정상관.MCP-1가능삼여료DN혈관병변.
Objective To observe the change of intima/media thickness ratio and expression of inflammatory factors in renal small artery of diabetic rats, and to explore the correlations of intim/media ratio with inflammatory factors and vascular lesions of diabetic nephropathy (DN) rats. Methods Seventy healthy SD rats were randomly divided into diabetic nephropathy group (DN, n=40) and normal control group (N, n=30). DN rat model was induced by intraperitoneal injection of streptozotocin (STZ). Thirty-five DN rats were successfully established. N group received same dose of citrate buffer. Rats were sacrificed after 4, 12, 24 weeks respectively.The intima/media thickness ratio in renal small artery was detected by immunofluorescence. The monocyte chemoattractant protein-1 (MCP-1) protein and mRNA expression of renal small artery were detected by immunohistochemistry and in-situ hybridization at each time point. Results Blood glucose and urine protein excretion (24 h) at different time points in DN group were significantly higher than those of N group (P＜0.05). From the 12th week, Scr, BUN, serum phosphorus were significantly higher than those of N group (P＜0.05). At the 4th week, renal small artery had the expression of MCP-1 protein and mRNA. The expression increased gradually with time, reached the highest at the 24th week, and was significantly higher than that of N group at each time point (P＜0.05). Immunofluorescence results showed that as compared to N group, in the first 4 weeks, intima/media thickness ratio in DN group was not different, at the 12th week the ratio was higher but without significant difference, at the 24th week the ratio was significantly higher (P＜0.05). Small artery intima/media thickness ratio of DN group was positively correlated with MCP-1, cholesterol, triglyceride, serum phosphate (r=0.742, P＜0.01; r=0.740, P＜0.01; r=0.829, P＜0.01; r=0.580, P＜0.01). Conclusions The arterioles intima/media thickness ratio of early DN is significantly correlated with MCP-1, lipids and phosphorus. MCP-1 may be involved in the DN vascular disease.