CAJ | 학술논문

目的检测溃疡性结肠炎(UC)患者结肠肠黏膜TLR2蛋白及TLR2 mRNA的表达及其与UC临床活动度、内镜分级的关系.方法取47例UC患者结肠黏膜标本根据内镜及临床活动度进行分级,同时收集正常对照者结肠黏膜标本13例.用Western Blot技术和半定量反转录聚合酶链反应方法(RT-PCR)检测结肠黏膜中TLR2蛋白及TLR2 mRNA的表达.结果 UC患者结肠黏膜TLR2蛋白及TLR2mRNA的表达量高于正常对照者,且随着内镜下及临床病变分级的加重逐渐增加.结论 UC患者肠黏膜中TLR2及TLR2 mRNA的表达增加,且其表达均随临床及内镜下病变程度加重,表达逐渐增加,在一定程度上可以反映疾病的活动度.
목적 검측궤양성결장염(UC)환자결장장점막TLR2단백급TLR2 mRNA적표체급기여UC림상활동도、내경분급적관계.방법 취47례UC환자결장점막표본근거내경급림상활동도진행분급,동시수집정상대조자결장점막표본13례.용Western Blot기술화반정량반전록취합매련반응방법(RT-PCR)검측결장점막중TLR2단백급TLR2 mRNA적표체.결과 UC환자결장점막TLR2단백급TLR2mRNA적표체량고우정상대조자,차수착내경하급림상병변분급적가중축점증가.결론 UC환자장점막중TLR2급TLR2 mRNA적표체증가,차기표체균수림상급내경하병변정도가중,표체축점증가,재일정정도상가이반영질병적활동도.
Objective To investigate the expression of TLR2 in colon mucosa of ulcerative colitis (UC) and to analyze the correlation with clinical activity and endoscopic grading. Methods The biopsies from 47 UC patients and 13 healthy controls were collected, and the expression of TLR2 protein and mRNA in colonic mucosa was determined by Western Blot and semi-quantitative RT-PCR, respectively. The patients were graded according to endoscopic and clinical findings. Results The expressions of TLR2 protein and TLR2 mRNA in UC patients were significantly increased than those in healthy controls, which was correlated with the progression of the disease. Conclusion The expressions of TLR2 protein and TLR2 mRNA in colon mucosa from UC patients might be used as a marker for disease activity.