CAJ | 학술논문

目的：探讨更昔洛韦(GCV)对人白血病细胞的抗增殖和诱导分化作用。方法：GCV加人K562细胞培养4 d，测定其活细胞数目、克隆形成率、联苯胺染色阳性率，观察光镜形态、组织化学染色，并进行流式细胞分析、端粒酶检测。结果：在GCV的作用下，K562细胞的增殖受抑，生长分数降低，并且向具有合成血红蛋白能力的细胞分化。结论：GCV对562细胞具有抑制增殖和诱导分化作用，该结果为慢性粒细胞白血病(CML)的治疗探索新的途径。
목적：탐토경석락위(GCV)대인백혈병세포적항증식화유도분화작용。방법：GCV가인K562세포배양4 d，측정기활세포수목、극륭형성솔、련분알염색양성솔，관찰광경형태、조직화학염색，병진행류식세포분석、단립매검측。결과：재GCV적작용하，K562세포적증식수억，생장분수강저，병차향구유합성혈홍단백능력적세포분화。결론：GCV대562세포구유억제증식화유도분화작용，해결과위만성립세포백혈병(CML)적치료탐색신적도경。
Objective: To explore whether gancyclovir (GCV) can inhibit the proliferation and induce the erythro-differ-entiation of the K562 human myeloid leukemia cell line. Methods: 562 cells were cultured with GCV for 4 days to detect cellular changes cloning efficiency, benzidine-positive rate, flow eytometry analysis, and telomerase activity. Results: When 562 cells grew in the medium containing GCV, the cellular growth and division were gradually suppressed,growth fracture decreased and further differentiation towards the cell producing hemoglobins was found. Conclusion: GCVcan inhibit proliferation and induce erythro-differentiation of K562 cells.