CAJ | 학술논문

采用RT-PCR法和3',5'RACE(Rapid amplification of cDNA ends)法从淇河鲫脑垂体RNA克隆出生长激素(Growthhormone,GH)cDNA.此cDNA全长1 191 nt[含Poly(A)14 nt],其5'端非编码区长55 nt,阅读框(Open reading frame,OAF)长633 nt,3'端非编码区长503 nt.由其推导的GH前体由210个氨基酸组成,其中氮端前22个氨基酸为信号肽部分.氨基酸序列比较表明,淇河鲫与同目的鲫鱼、鲤鱼、团头鲂和斑马鱼的同源性分别为98.6%、96.2%、91.5%和88.6%;与不同目的胡子鲇和鳗鲡的同源性分别为74.6%和49.5%;与哺乳类的家鼠和人等的同源性低于40%.图3参30
채용RT-PCR법화3',5'RACE(Rapid amplification of cDNA ends)법종기하즉뇌수체RNA극륭출생장격소(Growthhormone,GH)cDNA.차cDNA전장1 191 nt[함Poly(A)14 nt],기5'단비편마구장55 nt,열독광(Open reading frame,OAF)장633 nt,3'단비편마구장503 nt.유기추도적GH전체유210개안기산조성,기중담단전22개안기산위신호태부분.안기산서렬비교표명,기하즉여동목적즉어、리어、단두방화반마어적동원성분별위98.6%、96.2%、91.5%화88.6%;여불동목적호자점화만려적동원성분별위74.6%화49.5%;여포유류적가서화인등적동원성저우40%.도3삼30