CAJ | 학술논문

背景:绝大多数高分子材料在与血液接触时都导致不同程度凝血,使其应用受到限制.因此研制有优良抗凝血性能的高分子材料成为生物人工肝材料临床研究中的关键问题.目的:体外检测新型人工肝反应器材料--聚丙烯酰胺接枝改性聚丙烯膜(PP-g-AAm)的血液相容性.方法:对改性前、后的聚丙烯膜行溶血试验、凝血酶原时间和活化部分凝血激酶时间试验,用流式细胞术检测血小板CD62P和CD63的表达率,扫描电镜观察两种膜上血小板的黏附情况.结果与结论:聚丙烯膜和PP-g-AAm膜的溶血率分别为1.32%和1.46%;聚丙烯膜的凝血酶原时间和活化部分凝血激酶时间较PP-g-AAm 膜明显缩短(P<0.05);PP-g-AAm 膜激活血小板表达CD62P、CD63的百分率都明显少于聚丙烯膜(P<0.05);扫描电镜观察两种材料表面黏附的血小板都有明显变形,但PP-g-AAm膜表面黏附的血小板明显少于聚丙烯膜.提示PP-g-AAm膜具有良好的血液相容性.
배경:절대다수고분자재료재여혈액접촉시도도치불동정도응혈,사기응용수도한제.인차연제유우량항응혈성능적고분자재료성위생물인공간재료림상연구중적관건문제.목적:체외검측신형인공간반응기재료--취병희선알접지개성취병희막(PP-g-AAm)적혈액상용성.방법:대개성전、후적취병희막행용혈시험、응혈매원시간화활화부분응혈격매시간시험,용류식세포술검측혈소판CD62P화CD63적표체솔,소묘전경관찰량충막상혈소판적점부정황.결과여결론:취병희막화PP-g-AAm막적용혈솔분별위1.32%화1.46%;취병희막적응혈매원시간화활화부분응혈격매시간교PP-g-AAm 막명현축단(P<0.05);PP-g-AAm 막격활혈소판표체CD62P、CD63적백분솔도명현소우취병희막(P<0.05);소묘전경관찰량충재료표면점부적혈소판도유명현변형,단PP-g-AAm막표면점부적혈소판명현소우취병희막.제시PP-g-AAm막구유량호적혈액상용성.
BACKGROUND: Contacting with blood, most of polymer materials lead to different extents of blood coagulation, which limits their clinical application. Therefore, developing polymer materials with excellent anticoagulant property has become a key to clinical study of bioartificial liver materials.OBJECTIVE: To in vitro detect the blood dompatibility of polyacrylamide grafted polypropylene (PP) membrane (PP-g-AAm), a novel artificial liver reactor material.METHODS: Prior to and after modification, hemolytic test, prothrombin time and activated partial thromboplastin time tests of PP membrane were performed; blood platelet CD62P and CD63 expression rates were determined by flow cytometry, and platelet adhesion on PP and PP-g-AAm membranes by scanning electron microscopy.RESULTS AND CONCLUSION: The hemolysis ratio of PP and PP-g-AAm membranes was 1.32% and 1.46%, respectively.Compared with PP-g-AAm membrane, prothrombin time and activated partial thromboplastin time of PP membrane weremarkedly shorter (P < 0.05). CD62P and CD63 expression rates in the PP-g-AAm membrane were significantly lower than PP membrane (P < 0.05). Scanning electron microscopy results revealed that there were obvious changes of platelets adhering to these two membranes, but platelets adhering to PP-g-AAm membrane were fewer than PP membrane. These results indicate that PP-g-AAm membrane exhibits good blood compatibility.