糖尿病及其视网膜病变患者氧化应激状态的评价
당뇨병급기시망막병변환자양화응격상태적평개
Study of Oxidative Stress Status in Patients with Diabetes Mellitus and Diabetic Retinopathy
  • 万方数据
    中国全科医学 중국전과의학
    CHINESE GENERAL PRACTICE
    2010年 11期 1173-1175 ,共3页

    陈文华%郭欣%邢燕%郭美宜%许风娟%常东%潘洪志

    진문화%곽흔%형연%곽미의%허풍연%상동%반홍지

    糖尿病,2型%糖尿病视网膜病变%氧化应激 糖尿病,2型%糖尿病視網膜病變%氧化應激
    당뇨병,2형%당뇨병시망막병변%양화응격
    Diabetes mellitus,type 2%Diabetic retinopathy%Oxidative stress
    目的 观察糖尿病及其视网膜病变患者血清抗氧化酶活性及非酶类抗氧化物、氧化应激代谢产物的水平,探讨氧化应激与糖尿病视网膜病变之间的关系.方法 选择51例2型糖尿病患者,其中糖尿病无并发症者24例(DM组),糖尿病合并视网膜病变者27例(DR组),另选择同期行体检的30例健康者为对照组.收集3组受检者的空腹血清,测定血清抗氧化酶[超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及过氧化氢酶(CAT)]活性、非酶类抗氧化物[维生素C(V_C)、维生素E(V_E)及还原型谷胱甘肽(GSH)]水平及氧化应激代谢产物[丙二醛(MDA)、共轭二烯(CD)及晚期蛋白质氧化产物(AOPP)]水平.结果 (1)DM组患者血清SOD、GSH-Px、CAT活性分别为(108±14)U/ml、(141±30)U/ml和(5.6±1.6)U/ml;DR组患者分别为(96±13)U/ml、(133±25)U/ml和(5.1±1.4)U/ml;对照组分别为(119±28)U/ml、(156±26)U/ml和(6.8±1.6)U/ml;与对照组比较,DM组及DR组患者血清抗氧化酶活性均显著降低(P＜0.05),且DR组患者SOD活性又显著低于DM组患者(P＜0.05).(2)对照组V_C、V_E、GSH水平分别为(86±26)mg/L、(19.0±5.3)mg/L和(22.0±5.9)μmol/L;DM组患者分别为(64±20)mg/L、(16.6±3.7)mg/L和(20.8±8.2)μmol/L;DR组患者分别为(52±10)mg/L、(16.6±3.7)mg/L和(19.7±5.7)μmol/L;与对照组比较,DM组及DR组患者血清V_C水平均显著降低(P＜0.01),而DR组患者血清V_C水平又显著低于DM组(P＜0.05).(3)对照组血清MDA、CD、AOPP水平分别为(3.1±1.3)μmol/L、(0.32±0.06)和(15.8±4.5)μmol/L;DM组患者分别为(5.4±1.8)μmol/L、(0.43±0.23)和(25.6±7.7)μmol/L;DR组患者分别为(7.9±2.6)μmol/L、(0.57±0.27)和(27.8±7.2)μmol/L;与对照组比较,糖尿病患者氧化应激代谢产物水平显著升高(P＜0.05),且DR组患者血清MDA、CD水平较DM组患者显著升高(P＜0.05).结论 糖尿病及其视网膜病变患者存在着严重的氧化应激,且糖尿病视网膜病变患者氧化应激更严重.
    목적 관찰당뇨병급기시망막병변환자혈청항양화매활성급비매류항양화물、양화응격대사산물적수평,탐토양화응격여당뇨병시망막병변지간적관계.방법 선택51례2형당뇨병환자,기중당뇨병무병발증자24례(DM조),당뇨병합병시망막병변자27례(DR조),령선택동기행체검적30례건강자위대조조.수집3조수검자적공복혈청,측정혈청항양화매[초양화물기화매(SOD)、곡광감태과양화물매(GSH-Px)급과양화경매(CAT)]활성、비매류항양화물[유생소C(V_C)、유생소E(V_E)급환원형곡광감태(GSH)]수평급양화응격대사산물[병이철(MDA)、공액이희(CD)급만기단백질양화산물(AOPP)]수평.결과 (1)DM조환자혈청SOD、GSH-Px、CAT활성분별위(108±14)U/ml、(141±30)U/ml화(5.6±1.6)U/ml;DR조환자분별위(96±13)U/ml、(133±25)U/ml화(5.1±1.4)U/ml;대조조분별위(119±28)U/ml、(156±26)U/ml화(6.8±1.6)U/ml;여대조조비교,DM조급DR조환자혈청항양화매활성균현저강저(P＜0.05),차DR조환자SOD활성우현저저우DM조환자(P＜0.05).(2)대조조V_C、V_E、GSH수평분별위(86±26)mg/L、(19.0±5.3)mg/L화(22.0±5.9)μmol/L;DM조환자분별위(64±20)mg/L、(16.6±3.7)mg/L화(20.8±8.2)μmol/L;DR조환자분별위(52±10)mg/L、(16.6±3.7)mg/L화(19.7±5.7)μmol/L;여대조조비교,DM조급DR조환자혈청V_C수평균현저강저(P＜0.01),이DR조환자혈청V_C수평우현저저우DM조(P＜0.05).(3)대조조혈청MDA、CD、AOPP수평분별위(3.1±1.3)μmol/L、(0.32±0.06)화(15.8±4.5)μmol/L;DM조환자분별위(5.4±1.8)μmol/L、(0.43±0.23)화(25.6±7.7)μmol/L;DR조환자분별위(7.9±2.6)μmol/L、(0.57±0.27)화(27.8±7.2)μmol/L;여대조조비교,당뇨병환자양화응격대사산물수평현저승고(P＜0.05),차DR조환자혈청MDA、CD수평교DM조환자현저승고(P＜0.05).결론 당뇨병급기시망막병변환자존재착엄중적양화응격,차당뇨병시망막병변환자양화응격경엄중.
    Objective To observe antioxidative enzymes,non-enzyme antioxidants and metabolite level of oxidative stress in T2DM patients with or without retinopathy to explore the relationship between oxidative stress and diabetic retinopathy.Methods Fifty-one T2DM patients were divided into groups DM (without complications,n=24) and DR (with retinopathy,n=27),and another 30 healthy subjects were enrolled as control group.Superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),catalase (CAT),vitamin C (V_C),vitamin E(V_E),glutathione (GSH),malondialdehyde (MDA),conjugated diene (CD) and advanced oxidation protein products(AOPP) were detected.Results The levels of SOD,GSH-Px,CAT activity of DM group (108±14 U/ml,141±30 U/ml,5.6±1.6 U/ml,respectively) and DR group (96±13 U/ml,133±25 U/ml,5.1±1.4 U/ml,respectively) decreased significantly,as compared with control (119±28 U/ml,156±26 U/ml,6.8±1.6 U/ml,respectively) (P＜0.05).SOD were significantly lower in DR group than in DM (P＜0.05).The levels of V_C,V_E,GSH in serum were 86±26 mg/L,19.0±5.3 mg/L,22.0±5.9 μmol/L,respectively,in control group,and 64±20 mg/L,16.6±3.7 mg/L,20.8±8.2 μmol/L,respectively,in DM patients,and 52±10 mg/L,16.6±3.7 mg/L,19.7±5.7 μmol/L,respectively,in DR group.As compared with control,V_C decreased in groups DM and DR (P＜0.01).And V_C was lower in DR group than in DM group (P＜0.05).MDA,CD and AOPP of groups DM (5.4±1.8 μmol/L,0.43±0.23,25.6±7.7 μmol/L,respectively) and DR (7.9±2.6 μmol/L,0.57±0.27,27.8±7.2 μmol/L,respectively) were significantly higher than those of control (3.1±1.3 μmol/L,0.32±0.06,15.8±4.5 μmol/L,respectively) (P＜0.05).The levels of MDA and CD in DR group elevated significantly as compared with DM group (P＜0.05).Conclusion Oxidative stress is severe in diabetic patients with or without retinopathy,but more severe in patients with retinopathy.
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