CAJ | 학술논문

siRNA诱导的基因沉默为肿瘤的基因治疗提供了新的有效手段,但外源性siRNA在体内的安全高效递送一直是亟待解决的关键问题.聚乙烯亚胺(PEI)及其共聚物能够包裹siRNA形成胶囊化的纳米粒,在体外多种肿瘤细胞实验、体内多种肿瘤模型经瘤内、腹腔或静脉途径应用中,对肿瘤细胞和移植瘤模型的生长都有良好的抑制效果.纳米粒中siRNA的稳定性和生物活性与PEI的结构、分子量、N/P比值以及构成共聚物的成份等密切相关.
siRNA유도적기인침묵위종류적기인치료제공료신적유효수단,단외원성siRNA재체내적안전고효체송일직시극대해결적관건문제.취을희아알(PEI)급기공취물능구포과siRNA형성효낭화적납미립,재체외다충종류세포실험、체내다충종류모형경류내、복강혹정맥도경응용중,대종류세포화이식류모형적생장도유량호적억제효과.납미립중siRNA적은정성화생물활성여PEI적결구、분자량、N/P비치이급구성공취물적성빈등밀절상관.
The gene silence induced by small interfering RNA (siR N A) provides a powerful tool for gene therapy of neoplasm. However, safe and efficient delivery of exogenous siRNA is still the key issue for clinical application. Polyethyenimines ( PEI ) and its copolymers can encapsulate siRNA into complex in the formation of nanoparticle wherein the stability and activity of siRNA are closely related to the structure and molecular weight of PEI, N/P ratio, and the composition of copolymers.The siRNA-encapsulated nanoparticles can effectively inhibit the proliferation of tumor cell not only in vitro, but also in vivo by administration via intratumoral,intraperitoneal or venous injections.