CAJ | 학술논문

目的探讨支气管哮喘患者呼出气冷凝液(EBC)中半胱氨酰白三烯C4(LTC4)及8异前列腺素水平与病情的关系.方法对哮喘患者观察组30例(哮喘慢性持续期患者16例,哮喘临床缓解期14例)用自行设计的EBC收集仪器采集标本,采用酶联免疫法测定EBC中LTC4及8异前列腺素水平.结果观察组中哮喘持续期及缓解期LTC4水平[(67.38±10.30) ng/ml、(41.21±11.41)ng/ml]均高于正常对照组[(17.15±7.48) ng/ml,P＜0.01]；哮喘持续期LTC4水平高于缓解期(P＜0.01).哮喘持续期和缓解期8异前列腺素水平[( 14.29±10.74) ng/ml、(12.40±7.45) ng/ml]显著高于正常对照组[(6.93±5.58) ng/ml](P＜0.01或P＜0.05)；哮喘持续期8异前列腺素水平高于缓解期,其差异无统计学意义(P＞0.05).结论哮喘慢性持续期和缓解期均有持续气道炎症存在,呼出气冷凝液(EBC)中LTC4水平与哮喘病情严重度有关,监测患者EBC中LTC4水平有助于哮喘的病情判断及指导治疗.
목적 탐토지기관효천환자호출기냉응액(EBC)중반광안선백삼희C4(LTC4)급8이전렬선소수평여병정적관계.방법 대효천환자관찰조30례(효천만성지속기환자16례,효천림상완해기14례)용자행설계적EBC수집의기채집표본,채용매련면역법측정EBC중LTC4급8이전렬선소수평.결과 관찰조중효천지속기급완해기LTC4수평[(67.38±10.30) ng/ml、(41.21±11.41)ng/ml]균고우정상대조조[(17.15±7.48) ng/ml,P＜0.01]；효천지속기LTC4수평고우완해기(P＜0.01).효천지속기화완해기8이전렬선소수평[( 14.29±10.74) ng/ml、(12.40±7.45) ng/ml]현저고우정상대조조[(6.93±5.58) ng/ml](P＜0.01혹P＜0.05)；효천지속기8이전렬선소수평고우완해기,기차이무통계학의의(P＞0.05).결론 효천만성지속기화완해기균유지속기도염증존재,호출기냉응액(EBC)중LTC4수평여효천병정엄중도유관,감측환자EBC중LTC4수평유조우효천적병정판단급지도치료.
Objective To study the relationship between the level of LTC4,8-lsoprostane in exhaled breath condensate and the serious condition of patients with asthma.Methods 30 patients with asthma were enrolled in this study,16 cases were chronic persistent and 14 cases were clinical relieve.EBC was collected in all subjects with a self-designed experimental device.The concentrations of LTC4 and 8-Isoprostane in EBC were measured by enzyme immunoassay.Results Both LTC4 [ ( 67.38 ± 10.30) ng/ml,(41.21 ± 11.41 ) ng/ml ] and 8-Isoprostane levels[ ( 14.29 ± 10.74) ng/ml,( 12.40 ± 7.45 ) ng/ml ]were significantly increased in the asthma patients compared with the control subjects[ (17.15 ±7.48 )ng/ml,P ＜0.01 ].Exhaled LTC4 levels were obviously increased in the patients with chronic persistent asthma compared with those clinical relieve asthma,while 8-Isoprostane level had no significant difference between two asthma groups ( P ＞ 0.05 ).Conclusions Airway inflammation were persistent in asthma patients.LTC4 and 8-Isoprostane levels in EBC were associated with the serious condition of asthma,which indicated inflammation indicators might be useful in monitoring serious condition and guiding treatment for asthma patients.