中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2009年
10期
989-992
,共4页
叶曦%李国伟%姚美琳%罗炜%苏丽琼
葉晞%李國偉%姚美琳%囉煒%囌麗瓊
협희%리국위%요미림%라위%소려경
巴尔通体%鼠形动物%分布%基因类型
巴爾通體%鼠形動物%分佈%基因類型
파이통체%서형동물%분포%기인류형
Bartonella%Rodent%Distribution%Genotype
目的 了解福建省沿海不同地区、不同气温条件下鼠形动物巴尔通体感染状况,并掌握其菌种基因型.方法 通过随机抽样,抽取福建省沿海6个地市各一个调查点,采用笼日法捕获鼠形动物,培养分离巴尔通体,疑似菌株通过PCR证实为巴尔通体,并通过gltA基因的379bp片段序列测定,构建生长发育树,确定巴尔通体属种,并分析各属种的地区和宿主分布.结果 捕获并培养分离鼠形动物1161只(份),分离到巴尔通体菌株188株,6种被检动物中有5种检出巴尔通体,感染的鼠形动物分属2目2属,分别是臭鼩鼱、褐家鼠、黄胸鼠、小家鼠、黑家鼠.鼠形动物总感染率为16.19%,其中臭鼩鼱最高(21.43%),其次为褐家鼠(13.54%),黄胸鼠(18.27%).构成东南地区的主要鼠形动物均列其中.调查6个沿海地市的鼠形动物均感染巴尔通体菌,感染率分别为宁德9.25%、福州9.52%、莆田9.38%、泉州28.18%、厦门17.42%、漳州13.33%.比较年积温7000℃以下地区与年积温7000℃以上地区鼠形动物感染率差异有统计学意义(χ~2=12.93,P<0.001).序列分析表明福建省沿海地区鼠形动物中共检出3个巴尔通体种群:B.elizabethae、B.qeenslandensis和B,tribocorum A、B群,有明显的地域和宿主分布特征.结论 巴尔通体在福建省沿海地区鼠形动物中广泛存在,优势菌种与云南、北京等地区不尽相同.
目的 瞭解福建省沿海不同地區、不同氣溫條件下鼠形動物巴爾通體感染狀況,併掌握其菌種基因型.方法 通過隨機抽樣,抽取福建省沿海6箇地市各一箇調查點,採用籠日法捕穫鼠形動物,培養分離巴爾通體,疑似菌株通過PCR證實為巴爾通體,併通過gltA基因的379bp片段序列測定,構建生長髮育樹,確定巴爾通體屬種,併分析各屬種的地區和宿主分佈.結果 捕穫併培養分離鼠形動物1161隻(份),分離到巴爾通體菌株188株,6種被檢動物中有5種檢齣巴爾通體,感染的鼠形動物分屬2目2屬,分彆是臭鼩鼱、褐傢鼠、黃胸鼠、小傢鼠、黑傢鼠.鼠形動物總感染率為16.19%,其中臭鼩鼱最高(21.43%),其次為褐傢鼠(13.54%),黃胸鼠(18.27%).構成東南地區的主要鼠形動物均列其中.調查6箇沿海地市的鼠形動物均感染巴爾通體菌,感染率分彆為寧德9.25%、福州9.52%、莆田9.38%、泉州28.18%、廈門17.42%、漳州13.33%.比較年積溫7000℃以下地區與年積溫7000℃以上地區鼠形動物感染率差異有統計學意義(χ~2=12.93,P<0.001).序列分析錶明福建省沿海地區鼠形動物中共檢齣3箇巴爾通體種群:B.elizabethae、B.qeenslandensis和B,tribocorum A、B群,有明顯的地域和宿主分佈特徵.結論 巴爾通體在福建省沿海地區鼠形動物中廣汎存在,優勢菌種與雲南、北京等地區不儘相同.
목적 료해복건성연해불동지구、불동기온조건하서형동물파이통체감염상황,병장악기균충기인형.방법 통과수궤추양,추취복건성연해6개지시각일개조사점,채용롱일법포획서형동물,배양분리파이통체,의사균주통과PCR증실위파이통체,병통과gltA기인적379bp편단서렬측정,구건생장발육수,학정파이통체속충,병분석각속충적지구화숙주분포.결과 포획병배양분리서형동물1161지(빈),분리도파이통체균주188주,6충피검동물중유5충검출파이통체,감염적서형동물분속2목2속,분별시취구정、갈가서、황흉서、소가서、흑가서.서형동물총감염솔위16.19%,기중취구정최고(21.43%),기차위갈가서(13.54%),황흉서(18.27%).구성동남지구적주요서형동물균렬기중.조사6개연해지시적서형동물균감염파이통체균,감염솔분별위저덕9.25%、복주9.52%、보전9.38%、천주28.18%、하문17.42%、장주13.33%.비교년적온7000℃이하지구여년적온7000℃이상지구서형동물감염솔차이유통계학의의(χ~2=12.93,P<0.001).서렬분석표명복건성연해지구서형동물중공검출3개파이통체충군:B.elizabethae、B.qeenslandensis화B,tribocorum A、B군,유명현적지역화숙주분포특정.결론 파이통체재복건성연해지구서형동물중엄범존재,우세균충여운남、북경등지구불진상동.
Objective To investigate Bartonella infection in rodent hosts from different environments and types of climate in Fujian coastal regions. Genetypes of the Bartonella strains was also studied to provide scientific basis for prevention and control of the correlated diseases. Methods By random sampling method, we selected six study sites in Fujian southeastern coastal regions. Rodents were captured by cages to Isolate Bartonella strains. Bartonella-like isolates were confirmed by polymerase chain reaction (PCR). The 379 bp fragment of gltA gene was sequenced and the growth and development tree was constructed to determine Bartonella species. Distribution of Bartonella species in the different area and related hosts was also analysed. Results Bartonella species were isolated from 188 of 1161 small animals including five rodent species. The infected animals were grouped into 2 genera and 2 orders. They were Suncus murinus, Rattas norvegicus, Rnttus flavipectus, Mus masculus and Rattus rattus. The overall prevalence of Bartonella bacteremia was 16.19% in the most prevalent species of rodents in Fujian southeastern coastal regions including 21.43% in Suncus murinus, 13.54% in Rattas norvegicus and 18.27% in Rattus flavipectus. Rodents in every investigated areas were infected by Bartonella species (9.25% in Ningde, 9.52% in Fuzhou, 9.38% in Putian, 28.18% in Quanzhou, 17.42% in Xiamen and 13.33% in Zhangzhou). There were significant differences among infected rates in different annual accumulated temperature districts (χ~2=12.93, P<0.001). Isolates from rodents were clustered in three genotypes (B.elizabethae, B.qeenslandensis and B.tribocorum A, B). Conclusion The local rodents in Fujian southeastern coastal regions were widely infected by Bartonella spp. Differences among the prevalent species of Bartonella in Fujian southeastern coastal region, Yunan and Beijing were noticed. Our findings suggested there was a need to study the prevalence, related vectors and the molecular organism of Bartonella spp.
