实用儿科临床杂志
實用兒科臨床雜誌
실용인과림상잡지
Journal of Applied Clinical Pediatrics
2009年
5期
392-394
,共3页
丹参酮ⅡA%肾间质纤维化%转化生长因子-β1
丹參酮ⅡA%腎間質纖維化%轉化生長因子-β1
단삼동ⅡA%신간질섬유화%전화생장인자-β1
TanshinoneⅡA%renal interstitial fibrosis%transforming growth factor-β1
目的 探讨丹参酮ⅡA对大鼠肾间质纤维化的作用及可能机制.方法 雄性SD大鼠30只随机分为假手术组、模型组和丹参酮ⅡA组.假手术组仅找到输尿管,不行结扎术.模型组结扎大鼠左侧输尿管建立肾间质纤维化动物模型.丹参酮ⅡA组术前24 h经腹腔注射丹参酮ⅡA,剂量为1.5 mg/(kg*d),手术操作同模型组.术后第10天取梗阻侧肾组织行HE、Masson染色,观察其肾间质病理改变.免疫组织化学法检测其肾组织转化生长因子-β1(TGF-β1)、α-平滑肌肌动蛋白(α-SMA)及Ⅰ型胶原的蛋白表达与定位.反转录-聚合酶链反应(RT-PCR)检测3者mRNA水平.结果 输尿管结扎术后第10天梗阻侧出现明显的肾间质纤维化,肾组织TGF-β1、α-SMA和Ⅰ型胶原的蛋白及mRNA表达均显著增加(Pa<0.01).经丹参酮ⅡA治疗后,肾间质纤维化程度减轻,肾组织TGF-β1、α-SMA及Ⅰ型胶原表达均下调(Pa<0.05).结论 丹参酮ⅡA对大鼠肾间质纤维化有明显的抑制作用,其机制可能与下调TGF-β1表达,抑制成纤维细胞增殖和表型转化,减少胶原沉积有关.
目的 探討丹參酮ⅡA對大鼠腎間質纖維化的作用及可能機製.方法 雄性SD大鼠30隻隨機分為假手術組、模型組和丹參酮ⅡA組.假手術組僅找到輸尿管,不行結扎術.模型組結扎大鼠左側輸尿管建立腎間質纖維化動物模型.丹參酮ⅡA組術前24 h經腹腔註射丹參酮ⅡA,劑量為1.5 mg/(kg*d),手術操作同模型組.術後第10天取梗阻側腎組織行HE、Masson染色,觀察其腎間質病理改變.免疫組織化學法檢測其腎組織轉化生長因子-β1(TGF-β1)、α-平滑肌肌動蛋白(α-SMA)及Ⅰ型膠原的蛋白錶達與定位.反轉錄-聚閤酶鏈反應(RT-PCR)檢測3者mRNA水平.結果 輸尿管結扎術後第10天梗阻側齣現明顯的腎間質纖維化,腎組織TGF-β1、α-SMA和Ⅰ型膠原的蛋白及mRNA錶達均顯著增加(Pa<0.01).經丹參酮ⅡA治療後,腎間質纖維化程度減輕,腎組織TGF-β1、α-SMA及Ⅰ型膠原錶達均下調(Pa<0.05).結論 丹參酮ⅡA對大鼠腎間質纖維化有明顯的抑製作用,其機製可能與下調TGF-β1錶達,抑製成纖維細胞增殖和錶型轉化,減少膠原沉積有關.
목적 탐토단삼동ⅡA대대서신간질섬유화적작용급가능궤제.방법 웅성SD대서30지수궤분위가수술조、모형조화단삼동ⅡA조.가수술조부조도수뇨관,불행결찰술.모형조결찰대서좌측수뇨관건립신간질섬유화동물모형.단삼동ⅡA조술전24 h경복강주사단삼동ⅡA,제량위1.5 mg/(kg*d),수술조작동모형조.술후제10천취경조측신조직행HE、Masson염색,관찰기신간질병리개변.면역조직화학법검측기신조직전화생장인자-β1(TGF-β1)、α-평활기기동단백(α-SMA)급Ⅰ형효원적단백표체여정위.반전록-취합매련반응(RT-PCR)검측3자mRNA수평.결과 수뇨관결찰술후제10천경조측출현명현적신간질섬유화,신조직TGF-β1、α-SMA화Ⅰ형효원적단백급mRNA표체균현저증가(Pa<0.01).경단삼동ⅡA치료후,신간질섬유화정도감경,신조직TGF-β1、α-SMA급Ⅰ형효원표체균하조(Pa<0.05).결론 단삼동ⅡA대대서신간질섬유화유명현적억제작용,기궤제가능여하조TGF-β1표체,억제성섬유세포증식화표형전화,감소효원침적유관.
Objective To investigate the effects of TanshinoneⅡA on renal interstitial fibrosis in rats and its mechanism.Methods Thirty male Sprague-Dawley rats were randomly divided into sham-operated group,model group and TanshinoneⅡA treated group.Rats in sham operated group did not have their left ureteral obstructed.Animal models of renal interstitial fibrosis were established with unilateral ureteral obstruction (UUO) in rats.TanshinoneⅡA was intraperitoneally injected every day with dose of 1.5 mg/kg on 24 hours before operation in TanshinoneⅡA treated group.The operation procedure in TanshinoneⅡA treated group was the same as that in the model group.After 10 days of UUO,all rats were killed and the obstructed kidney were collected for Hematoxylin-Eosin (HE) and Masson staining to check the pathological changes of renal interstitial tissues.The protein expression and localization of transforming growth factor-β1(TGF-β1),α-smooth muscle actin(α-SMA) and typeⅠcollagen in renal tissues were detected with immunohistochemical staining.The mRNA levels of TGF-β1,α-SMA and typeⅠcollagen were analyzed with reverse transcription-polymerase chain reaction(RT-PCR).Results Compared with sham-operated group,model group exhibited severe renal interstitial fibrosis and the expressions of TGF-β1,α-SMA and typeⅠcollagen were markedly higher in obstructed kidney(Pa<0.01).Treated with TanshinoneⅡA,renal interstitial fibrosis was ameliorated and the expression levels of TGF-β1,α-SMA and typeⅠcollagen were down-regulated significantly in renal tissues(Pa<0.05).Conclusion TanshinoneⅡA can inhibit renal interstitial fibrosis by down-regulating TGF-β1 expression,inhibiting fibroblasts proliferation and phenotypic transitions and reducing collagen sediment.
