中国汉族人群TCF7L2基因多态性与2型糖尿病的关联研究
중국한족인군TCF7L2기인다태성여2형당뇨병적관련연구
Association of polymorphisms in transcription factor 7-like 2(TCF7L2) gene with type 2 diabetes in Chinese Han population
  • 万方数据
    中华内分泌代谢杂志 중화내분비대사잡지
    CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
    2009年 2期 139-143 ,共5页

    汪志红%张素华%王增产%龚莉琳%李蓉%任伟%郑瑞芝%汪茂荣%张文露%程庆丰%梁松%万小莉%张兰英%何军

    왕지홍%장소화%왕증산%공리림%리용%임위%정서지%왕무영%장문로%정경봉%량송%만소리%장란영%하군

    多态性,单核苷酸%TCF7L2基因%糖尿病,2型%关联研究 多態性,單覈苷痠%TCF7L2基因%糖尿病,2型%關聯研究
    다태성,단핵감산%TCF7L2기인%당뇨병,2형%관련연구
    Polymorphism,single nucleotide%TCF7L2 gene%Diabetes mellitus,type 2%Association study
    目的 研究中国汉族人群TCF7L2(transcription factor 7-like 2)基因单核苷酸多态性(SNP)与2型精尿病的相关性.方法 应用限制性片段长度多态性(RFLP)方法 ,对749例重庆及周边地区汉族人(其中2刭糖尿病患者446例、正常对照303名)的rs7903146和rs12255372进行基因分型;同时进行人体测量学及代谢指标的检测,并用稳态模型评估胰岛素抵抗(HOMA-IR)和胰岛13细胞功能(HOMA-p). 结果 (1)rs7903146位点的T等位基因频率、CT和TT基因型频率在2型糖尿病组均明显高于正常对照组(分别为0.093、0.150、0.018和0.043、0.079、0.003,均P<0.01);与CC型相比,CT/TT基因型患2型糖尿病的危险显著增加(OR=2.25,95%C/为1.39～3.62,P=O.001);CT/TT基因型与胰岛素分泌减少有关.(2)rs12255372位点G、T等位基因及基因型频率分布在正常对照组、2型糖尿病组中均无显著性差异.结论 TCFTL2基因rs7903146多态性位点的T等位基因是2型糖尿病的风险等位基因,提示TCF7L2基因可能是中国汉族人2型糖尿病的易感基因之一.
    목적 연구중국한족인군TCF7L2(transcription factor 7-like 2)기인단핵감산다태성(SNP)여2형정뇨병적상관성.방법 응용한제성편단장도다태성(RFLP)방법 ,대749례중경급주변지구한족인(기중2경당뇨병환자446례、정상대조303명)적rs7903146화rs12255372진행기인분형;동시진행인체측량학급대사지표적검측,병용은태모형평고이도소저항(HOMA-IR)화이도13세포공능(HOMA-p). 결과 (1)rs7903146위점적T등위기인빈솔、CT화TT기인형빈솔재2형당뇨병조균명현고우정상대조조(분별위0.093、0.150、0.018화0.043、0.079、0.003,균P<0.01);여CC형상비,CT/TT기인형환2형당뇨병적위험현저증가(OR=2.25,95%C/위1.39～3.62,P=O.001);CT/TT기인형여이도소분비감소유관.(2)rs12255372위점G、T등위기인급기인형빈솔분포재정상대조조、2형당뇨병조중균무현저성차이.결론 TCFTL2기인rs7903146다태성위점적T등위기인시2형당뇨병적풍험등위기인,제시TCF7L2기인가능시중국한족인2형당뇨병적역감기인지일.
    Objective To study the association of transcription factor 7-like 2(TCF7L2)polymorphisms with tvpe 2 diabetes mellitus in Chinese Han population. Methods Two polymorphisms (rs7903146 and rs12255372)of TCF7L2 gene were genotyped in 446 patients with type 2 diabetes mellitus(T2DM group)and 303 normal subiects (NC group) by PCR-restriction fragment length polymorphism(PCR-RFLP).Waist circumference.body mass index,plasma glucose,serum insulin,lipid profiles,high-sensitivity C-reactive protein and non-esterified fatty acid were measured.Homeostasis model assessment of insulin resistance(HOMA-IR)and β-cell function(HOMA-β)were calculated.Results (1) In T2DM group,T allele frequency and CT,TY geno tvpe frequeneies of rs7903146 were significantly higher than those in NC group(0.093,0.150,0.018 vs 0.043, 0.079,0.003,respectively,a11 P<O.O 1).Logistic regression analysis showed that the CT/TT genotype was a risk factor of tvpe 2 diabetes(OR=2.25,95%CI 1.39-3.62,P=0.001)and was associated with the decrease of insulin secretion. (2) No significant association was observed in vs12255372 alleles and genotypes with type 2 diabetes mellitus.Conclusion These results indicate that TCF7L2 might be one of the candidate genes for confe ring susceptibi lity to type 2 diabetes mellitus in the Chinese Han population.
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